Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars
Identifieur interne : 003627 ( Main/Merge ); précédent : 003626; suivant : 003628Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars
Auteurs : R. M. Perez [Espagne] ; L. Navarro [Espagne] ; N. Duran-Vila [Espagne]Source :
- Plant cell reports [ 0721-7714 ] ; 1997.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Agrume.
English descriptors
- KwdEn :
Abstract
Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconser-vation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R1 and solid phase R2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R1 not above 0.5°C min-1 and R2 not above 1°C min1) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleo-patra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000B02
- to stream PascalFrancis, to step Curation: 001405
- to stream PascalFrancis, to step Checkpoint: 000B21
Links to Exploration step
Pascal:98-0058421Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars</title>
<author><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">98-0058421</idno>
<date when="1997">1997</date>
<idno type="stanalyst">PASCAL 98-0058421 INIST</idno>
<idno type="RBID">Pascal:98-0058421</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000B02</idno>
<idno type="wicri:Area/PascalFrancis/Curation">001405</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000B21</idno>
<idno type="wicri:doubleKey">0721-7714:1997:Perez R:cryopreservation:and:storage</idno>
<idno type="wicri:Area/Main/Merge">003627</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars</title>
<author><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1>
<s2>46113 Moncada (Valencia)</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant cell reports</title>
<title level="j" type="abbreviated">Plant cell rep.</title>
<idno type="ISSN">0721-7714</idno>
<imprint><date when="1997">1997</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant cell reports</title>
<title level="j" type="abbreviated">Plant cell rep.</title>
<idno type="ISSN">0721-7714</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Callus</term>
<term>Citrus aurantifolia</term>
<term>Citrus aurantium</term>
<term>Citrus fruit</term>
<term>Citrus limon</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Cryogenic storage</term>
<term>Cryopreservation</term>
<term>Embryo</term>
<term>Embryo culture</term>
<term>Embryonic development</term>
<term>Freeze thaw cycle</term>
<term>Germplasm</term>
<term>Performance evaluation</term>
<term>Vegetals</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Embryon</term>
<term>Stockage cryogénique</term>
<term>Gel dégel</term>
<term>Culture embryon</term>
<term>Citrus paradisi</term>
<term>Citrus limon</term>
<term>Citrus aurantium</term>
<term>Citrus aurantifolia</term>
<term>Citrus sinensis</term>
<term>Cryoconservation</term>
<term>Cal</term>
<term>Développement embryonnaire</term>
<term>Evaluation performance</term>
<term>Végétal</term>
<term>Agrume</term>
<term>Germoplasme</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconser-vation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R<sub>1</sub>
and solid phase R<sub>2</sub>
cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R<sub>1</sub>
not above 0.5°C min<sup>-1</sup>
and R<sub>2</sub>
not above 1°C min<sup>1</sup>
) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C. sinensis Osb.), three cultivars of grapefruit (C. paradisi Macf.), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleo-patra mandarin (C. reshni Hort. ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
</country>
<region><li>Communauté valencienne</li>
</region>
</list>
<tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Perez, R M" sort="Perez, R M" uniqKey="Perez R" first="R. M." last="Perez">R. M. Perez</name>
</region>
<name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
<name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003627 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003627 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Merge |type= RBID |clé= Pascal:98-0058421 |texte= Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |