Alteration of V-type H+-ATPase during methyljasmonate-induced senescence in barley ( Hordeum vulgare L. cv. Salome)
Identifieur interne : 003465 ( Main/Merge ); précédent : 003464; suivant : 003466Alteration of V-type H+-ATPase during methyljasmonate-induced senescence in barley ( Hordeum vulgare L. cv. Salome)
Auteurs : R. Ratajczak [Allemagne] ; I. Feussner [Allemagne] ; B. Hause [Allemagne] ; A. Böhm [Allemagne] ; B. Parthier [Allemagne] ; C. Wasternack [Allemagne]Source :
- Journal of Plant Physiology [ 0176-1617 ] ; 1998.
English descriptors
- KwdEn :
Abstract
In barley leaves, the application of (−)-jasmonic acid or its methyl ester (JAME) induces a senescencelike phenotype. This is accompanied by the synthesis of abundant proteins, so-called jasmonate-induced proteins (JlPs). Here, we show that modifications of vacuolar H+-ATPase (V-ATPase) subunits are jasmo-nate inducible. Using immunofluorescence analysis, we demonstrate that V-ATPase of barley leaves is exclusively located at the tonoplast also upon JAME treatment. Total ATP-hydrolysis activity of microsomal fractions increased by a factor of 10 during 72 h of JAME-treatment, while Bafilomycin Ai-sensitive ATP-hydrolysis activity, which is usually referred to V-ATPase activity, increased by a factor of about 2 in tono-plast-enriched membrane fractions. Moreover, due to JAME treatment there was a pronounced increase in ATP-hydrolysis activity at pH 6.2. This activity was not affected by inhibitors of P-, F-, or V-ATPases. However, biochemical analysis of partially purified V-ATPase suggests, that this activity might be due at least in part to the V-ATPase. JAME-treatment seems to change biochemical properties of the V-ATPase, i.e. a shift of the pH optimum of activity to a more acidic pH and a decrease in Bafilomycin A1 sensitivity. This is accompanied by the appearance of several additional forms of V-ATPase subunits which might represent either different isoforms or post-translationally modified proteins. We suggest that these changes in properties of the V-ATPase, which is involved in house-keeping and stress responses, may be due to JAME-induced senescence to overcome concomitant changes of the vacuolar membrane.
Url:
DOI: 10.1016/S0176-1617(98)80133-8
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000749
- to stream Istex, to step Curation: 000749
- to stream Istex, to step Checkpoint: 000C59
Links to Exploration step
ISTEX:AA2F4CB65804434D1086F7A002E03B9C18400E28Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alteration of V-type H+-ATPase during methyljasmonate-induced senescence in barley ( Hordeum vulgare L. cv. Salome)</title><author><name sortKey="Ratajczak, R" sort="Ratajczak, R" uniqKey="Ratajczak R" first="R." last="Ratajczak">R. Ratajczak</name></author><author><name sortKey="Feussner, I" sort="Feussner, I" uniqKey="Feussner I" first="I." last="Feussner">I. Feussner</name></author><author><name sortKey="Hause, B" sort="Hause, B" uniqKey="Hause B" first="B." last="Hause">B. Hause</name></author><author><name sortKey="Bohm, A" sort="Bohm, A" uniqKey="Bohm A" first="A." last="Böhm">A. Böhm</name></author><author><name sortKey="Parthier, B" sort="Parthier, B" uniqKey="Parthier B" first="B." last="Parthier">B. Parthier</name></author><author><name sortKey="Wasternack, C" sort="Wasternack, C" uniqKey="Wasternack C" first="C." last="Wasternack">C. Wasternack</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AA2F4CB65804434D1086F7A002E03B9C18400E28</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0176-1617(98)80133-8</idno><idno type="url">https://api.istex.fr/document/AA2F4CB65804434D1086F7A002E03B9C18400E28/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000749</idno><idno type="wicri:Area/Istex/Curation">000749</idno><idno type="wicri:Area/Istex/Checkpoint">000C59</idno><idno type="wicri:doubleKey">0176-1617:1998:Ratajczak R:alteration:of:v</idno><idno type="wicri:Area/Main/Merge">003465</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Alteration of V-type H+-ATPase during methyljasmonate-induced senescence in barley ( Hordeum vulgare L. cv. Salome)</title><author><name sortKey="Ratajczak, R" sort="Ratajczak, R" uniqKey="Ratajczak R" first="R." last="Ratajczak">R. Ratajczak</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut fur Botanik, Technische Universität Darmstadt, Schnittspahnstr. 3-5, D-64287 Darmstadt</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Darmstadt</settlement></placeName></affiliation></author><author><name sortKey="Feussner, I" sort="Feussner, I" uniqKey="Feussner I" first="I." last="Feussner">I. Feussner</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut fur Botanik, Technische Universität Darmstadt, Schnittspahnstr. 3-5, D-64287 Darmstadt</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Darmstadt</settlement></placeName></affiliation></author><author><name sortKey="Hause, B" sort="Hause, B" uniqKey="Hause B" first="B." last="Hause">B. Hause</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiochemie, Weinberg 3, D-06120 Halle</wicri:regionArea><wicri:noRegion>06120 Halle</wicri:noRegion><wicri:noRegion>D-06120 Halle</wicri:noRegion></affiliation></author><author><name sortKey="Bohm, A" sort="Bohm, A" uniqKey="Bohm A" first="A." last="Böhm">A. Böhm</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiochemie, Weinberg 3, D-06120 Halle</wicri:regionArea><wicri:noRegion>06120 Halle</wicri:noRegion><wicri:noRegion>D-06120 Halle</wicri:noRegion></affiliation></author><author><name sortKey="Parthier, B" sort="Parthier, B" uniqKey="Parthier B" first="B." last="Parthier">B. Parthier</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiochemie, Weinberg 3, D-06120 Halle</wicri:regionArea><wicri:noRegion>06120 Halle</wicri:noRegion><wicri:noRegion>D-06120 Halle</wicri:noRegion></affiliation></author><author><name sortKey="Wasternack, C" sort="Wasternack, C" uniqKey="Wasternack C" first="C." last="Wasternack">C. Wasternack</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiochemie, Weinberg 3, D-06120 Halle</wicri:regionArea><wicri:noRegion>06120 Halle</wicri:noRegion><wicri:noRegion>D-06120 Halle</wicri:noRegion></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Plant Physiology</title><title level="j" type="abbrev">JPLPH</title><idno type="ISSN">0176-1617</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">152</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="199">199</biblScope><biblScope unit="page" to="206">206</biblScope></imprint><idno type="ISSN">0176-1617</idno></series><idno type="istex">AA2F4CB65804434D1086F7A002E03B9C18400E28</idno><idno type="DOI">10.1016/S0176-1617(98)80133-8</idno><idno type="PII">S0176-1617(98)80133-8</idno><idno type="ArticleID">80133</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0176-1617</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Altered subunits</term><term>BSA</term><term>DAPI</term><term>DTT</term><term>EDTA</term><term>FITC</term><term>HEPES</term><term>J1Ps</term><term>JAME</term><term>MES</term><term>PBS</term><term>PEG</term><term>SDS-PAGE</term><term>Tris</term><term>V-ATPase</term><term>biochemical properties</term><term>jasmonates</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">In barley leaves, the application of (−)-jasmonic acid or its methyl ester (JAME) induces a senescencelike phenotype. This is accompanied by the synthesis of abundant proteins, so-called jasmonate-induced proteins (JlPs). Here, we show that modifications of vacuolar H+-ATPase (V-ATPase) subunits are jasmo-nate inducible. Using immunofluorescence analysis, we demonstrate that V-ATPase of barley leaves is exclusively located at the tonoplast also upon JAME treatment. Total ATP-hydrolysis activity of microsomal fractions increased by a factor of 10 during 72 h of JAME-treatment, while Bafilomycin Ai-sensitive ATP-hydrolysis activity, which is usually referred to V-ATPase activity, increased by a factor of about 2 in tono-plast-enriched membrane fractions. Moreover, due to JAME treatment there was a pronounced increase in ATP-hydrolysis activity at pH 6.2. This activity was not affected by inhibitors of P-, F-, or V-ATPases. However, biochemical analysis of partially purified V-ATPase suggests, that this activity might be due at least in part to the V-ATPase. JAME-treatment seems to change biochemical properties of the V-ATPase, i.e. a shift of the pH optimum of activity to a more acidic pH and a decrease in Bafilomycin A1 sensitivity. This is accompanied by the appearance of several additional forms of V-ATPase subunits which might represent either different isoforms or post-translationally modified proteins. We suggest that these changes in properties of the V-ATPase, which is involved in house-keeping and stress responses, may be due to JAME-induced senescence to overcome concomitant changes of the vacuolar membrane.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003465 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003465 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:AA2F4CB65804434D1086F7A002E03B9C18400E28
|texte= Alteration of V-type H+-ATPase during methyljasmonate-induced senescence in barley ( Hordeum vulgare L. cv. Salome)
}}
| This area was generated with Dilib version V0.6.25. |  |