Brief communication. The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding
Identifieur interne : 003463 ( Main/Merge ); précédent : 003462; suivant : 003464Brief communication. The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding
Auteurs : Ml Roose ; T. Schwarzacher ; Js Heslop-HarrisonSource :
- Journal of Heredity [ 0022-1503 ] ; 1998-01.
Abstract
In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x =18). Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus sinensis X Poncirus trifoliata. (2n = 2x = 18). Counterstaining with the fluorochrome chomomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus X Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessories examined.
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DOI: 10.1093/jhered/89.1.83
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<front><div type="abstract" xml:lang="en">In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x =18). Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus sinensis X Poncirus trifoliata. (2n = 2x = 18). Counterstaining with the fluorochrome chomomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus X Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessories examined.</div>
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