Biochemical basis of color as an aesthetic quality in Citrus sinensis.
Identifieur interne : 002E88 ( Main/Merge ); précédent : 002E87; suivant : 002E89Biochemical basis of color as an aesthetic quality in Citrus sinensis.
Auteurs : R. Oberholster [Hongrie] ; A K Cowan ; P. Molnár ; G. T ThSource :
- Journal of agricultural and food chemistry [ 0021-8561 ] ; 2001.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : beta Carotene.
- chemical , analysis : Carotenoids, Pigments, Biological, beta Carotene.
- chemistry : Citrus.
- Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Color, Xanthophylls.
Abstract
The biochemical basis of color as an aesthetic quality in mature fruit of navel and Valencia orange (Citrus sinensis) was determined. Saponification of the two major color-imparting components resolved by thin-layer chromatography, followed by reversed-phase high-performance liquid chromatography, revealed that these comprised acyl esters of (9Z)-violaxanthin and beta-citraurin. Identification of the chromophores was based on cochromatography and online spectral analysis. The color quality of flavedo of mature fruit was dependent on the content and relative amounts of (9Z)-violaxanthin and beta-citraurin. Quantitative results revealed that increased color intensity was associated with a decline in the (9Z)-violaxanthin:beta-citraurin ratio from greater than 50 to below 10, an increase in flavedo (9Z)-violaxanthin and beta-citraurin content, and that measurement of the mass and ratio of these carotenoids can be used to accurately color-grade orange fruit for local and export markets.
PubMed: 11170592
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pubmed:11170592Le document en format XML
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<author><name sortKey="Oberholster, R" sort="Oberholster, R" uniqKey="Oberholster R" first="R" last="Oberholster">R. Oberholster</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Chemistry, Faculty of Medicine, Pécs University, Szigeti út 12, P.O. Box 99, H-7601, Hungary.</nlm:affiliation>
<country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Department of Medical Chemistry, Faculty of Medicine, Pécs University, Szigeti út 12, P.O. Box 99, H-7601</wicri:regionArea>
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<author><name sortKey="Cowan, A K" sort="Cowan, A K" uniqKey="Cowan A" first="A K" last="Cowan">A K Cowan</name>
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<author><name sortKey="Molnar, P" sort="Molnar, P" uniqKey="Molnar P" first="P" last="Molnár">P. Molnár</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Biochemical basis of color as an aesthetic quality in Citrus sinensis.</title>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Chemistry, Faculty of Medicine, Pécs University, Szigeti út 12, P.O. Box 99, H-7601, Hungary.</nlm:affiliation>
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<series><title level="j">Journal of agricultural and food chemistry</title>
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<term>Color</term>
<term>Pigments, Biological (analysis)</term>
<term>Xanthophylls</term>
<term>beta Carotene (analogs & derivatives)</term>
<term>beta Carotene (analysis)</term>
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<front><div type="abstract" xml:lang="en">The biochemical basis of color as an aesthetic quality in mature fruit of navel and Valencia orange (Citrus sinensis) was determined. Saponification of the two major color-imparting components resolved by thin-layer chromatography, followed by reversed-phase high-performance liquid chromatography, revealed that these comprised acyl esters of (9Z)-violaxanthin and beta-citraurin. Identification of the chromophores was based on cochromatography and online spectral analysis. The color quality of flavedo of mature fruit was dependent on the content and relative amounts of (9Z)-violaxanthin and beta-citraurin. Quantitative results revealed that increased color intensity was associated with a decline in the (9Z)-violaxanthin:beta-citraurin ratio from greater than 50 to below 10, an increase in flavedo (9Z)-violaxanthin and beta-citraurin content, and that measurement of the mass and ratio of these carotenoids can be used to accurately color-grade orange fruit for local and export markets.</div>
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