Somatic hybridisation for improvement of citrus rootstock: production of five new combinations with potential for improved disease resistance
Identifieur interne : 002C87 ( Main/Merge ); précédent : 002C86; suivant : 002C88Somatic hybridisation for improvement of citrus rootstock: production of five new combinations with potential for improved disease resistance
Auteurs : M. A. Pereira De Carvalho Costa [Brésil] ; B. M. J. Mendes [Brésil] ; F. A. A. Filho Mourao [Brésil]Source :
- Australian journal of experimental agriculture [ 0816-1089 ] ; 2003.
Descripteurs français
- Pascal (Inist)
- Amélioration génétique, Phytopathologie, Sensibilité résistance, Hybridation interspécifique, Hybridation intergénérique, Hybride somatique, Protoplaste, Culture tissu, Citrus reticulata, Citrus sinensis, Citrus volkameriana, Virus tristeza agrume, Marqueur RAPD, Phytophthora, Fortunella obovata, Citrus sunki, Citrus limonia.
English descriptors
- KwdEn :
Abstract
The development of biotechnological tools such as protoplast fusion has provided a means of bypassing natural barriers related to reproductive biology of citrus found in conventional breeding systems. We report the production of 4 interspecific somatic hybrids, Cleopatra mandarin + Volkamer lemon, Ruby Blood sweet orange + Volkamer lemon, Rohde Red Valencia sweet orange + Volkamer lemon, and Rangpur lime + Sunki mandarin, and 1 intergeneric somatic hybrid, Valencia sweet orange + Fortunella obovata (Tanaka), which may be included and evaluated in rootstock improvement programs. Protoplasts were isolated from embryogenic calli and seedling leaves, and chemically fused with polyethylene glycol. Plants were regenerated via somatic embryogenesis and somatic hybridisation was confirmed by foliar morphology, cytological and DNA (RAPD) analyses. Somatic hybrids may combine required rootstock characteristics such as tolerance to citrus blight, tristeza virus, and Phytophthora, and have potential for control of tree size due to polyploidy.
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Pascal:04-0150802Le document en format XML
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<term>Genetic improvement</term>
<term>Intergeneric hybridization</term>
<term>Interspecific hybridization</term>
<term>Phytophthora</term>
<term>Plant pathology</term>
<term>Protoplast</term>
<term>Random amplified polymorphic DNA marker</term>
<term>Sensitivity resistance</term>
<term>Somatic hybrid</term>
<term>Tissue culture</term>
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<term>Phytopathologie</term>
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<term>Hybridation intergénérique</term>
<term>Hybride somatique</term>
<term>Protoplaste</term>
<term>Culture tissu</term>
<term>Citrus reticulata</term>
<term>Citrus sinensis</term>
<term>Citrus volkameriana</term>
<term>Virus tristeza agrume</term>
<term>Marqueur RAPD</term>
<term>Phytophthora</term>
<term>Fortunella obovata</term>
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<front><div type="abstract" xml:lang="en">The development of biotechnological tools such as protoplast fusion has provided a means of bypassing natural barriers related to reproductive biology of citrus found in conventional breeding systems. We report the production of 4 interspecific somatic hybrids, Cleopatra mandarin + Volkamer lemon, Ruby Blood sweet orange + Volkamer lemon, Rohde Red Valencia sweet orange + Volkamer lemon, and Rangpur lime + Sunki mandarin, and 1 intergeneric somatic hybrid, Valencia sweet orange + Fortunella obovata (Tanaka), which may be included and evaluated in rootstock improvement programs. Protoplasts were isolated from embryogenic calli and seedling leaves, and chemically fused with polyethylene glycol. Plants were regenerated via somatic embryogenesis and somatic hybridisation was confirmed by foliar morphology, cytological and DNA (RAPD) analyses. Somatic hybrids may combine required rootstock characteristics such as tolerance to citrus blight, tristeza virus, and Phytophthora, and have potential for control of tree size due to polyploidy.</div>
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