Application of probe electrospray to direct ambient analysis of biological samples.
Identifieur interne : 002145 ( Main/Merge ); précédent : 002144; suivant : 002146Application of probe electrospray to direct ambient analysis of biological samples.
Auteurs : Lee Chuin Chen [Japon] ; Kentaro Nishidate ; Yuta Saito ; Kunihiko Mori ; Daiki Asakawa ; Sen Takeda ; Takeo Kubota ; Nobuo Terada ; Yutaka Hashimoto ; Hirokazu Hori ; Kenzo HiraokaSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 2008.
English descriptors
- KwdEn :
- Animals, Biology (methods), Brain Chemistry, Breast Feeding, Cattle, Citrus sinensis (chemistry), Eggs (analysis), Female, Gramicidin (analysis), Humans, Insulin (analysis), Liver (chemistry), Mice, Milk (chemistry), Milk, Human (chemistry), Musa (chemistry), Salmon (anatomy & histology), Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization (instrumentation), Spectrometry, Mass, Electrospray Ionization (methods), Urine (chemistry).
- MESH :
- chemical , analysis : Gramicidin, Insulin.
- analysis : Eggs.
- anatomy & histology : Salmon.
- chemistry : Citrus sinensis, Liver, Milk, Milk, Human, Musa, Urine.
- instrumentation : Spectrometry, Mass, Electrospray Ionization.
- methods : Biology, Spectrometry, Mass, Electrospray Ionization.
- Animals, Brain Chemistry, Breast Feeding, Cattle, Female, Humans, Mice, Sensitivity and Specificity.
Abstract
Recently, we have developed probe electrospray ionization (PESI) that uses a solid needle. In this system, the probe needle moves up and down along the vertical axis by a motor-driven system. At the highest position of the probe needle, electrospray is generated by applying a high voltage. In this study, we applied PESI directly to biological samples such as urine, mouse brain, mouse liver, salmon egg, and fruits (orange, banana, etc.). Strong ion signals for almost all the samples were obtained. The amount of liquid sample picked up by the needle is as small as pL or less, making PESI a promising non-invasive technique for detecting biomolecules in living systems such as cells. Therefore, PESI may be useful as a versatile and ready-to-use semi-online analytical tool in the fields of medicine, pharmaceuticals, agriculture, food science, etc.
DOI: 10.1002/rcm.3626
PubMed: 18623622
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pubmed:18623622Le document en format XML
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<author><name sortKey="Chen, Lee Chuin" sort="Chen, Lee Chuin" uniqKey="Chen L" first="Lee Chuin" last="Chen">Lee Chuin Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Clean Energy Research Center, University of Yamanashi, Takeda- 4, Kofu 400-8511, Japan.</nlm:affiliation>
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<author><name sortKey="Nishidate, Kentaro" sort="Nishidate, Kentaro" uniqKey="Nishidate K" first="Kentaro" last="Nishidate">Kentaro Nishidate</name>
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<author><name sortKey="Saito, Yuta" sort="Saito, Yuta" uniqKey="Saito Y" first="Yuta" last="Saito">Yuta Saito</name>
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<author><name sortKey="Mori, Kunihiko" sort="Mori, Kunihiko" uniqKey="Mori K" first="Kunihiko" last="Mori">Kunihiko Mori</name>
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<author><name sortKey="Asakawa, Daiki" sort="Asakawa, Daiki" uniqKey="Asakawa D" first="Daiki" last="Asakawa">Daiki Asakawa</name>
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<author><name sortKey="Takeda, Sen" sort="Takeda, Sen" uniqKey="Takeda S" first="Sen" last="Takeda">Sen Takeda</name>
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<author><name sortKey="Kubota, Takeo" sort="Kubota, Takeo" uniqKey="Kubota T" first="Takeo" last="Kubota">Takeo Kubota</name>
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<author><name sortKey="Terada, Nobuo" sort="Terada, Nobuo" uniqKey="Terada N" first="Nobuo" last="Terada">Nobuo Terada</name>
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<author><name sortKey="Hashimoto, Yutaka" sort="Hashimoto, Yutaka" uniqKey="Hashimoto Y" first="Yutaka" last="Hashimoto">Yutaka Hashimoto</name>
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<author><name sortKey="Chen, Lee Chuin" sort="Chen, Lee Chuin" uniqKey="Chen L" first="Lee Chuin" last="Chen">Lee Chuin Chen</name>
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<author><name sortKey="Nishidate, Kentaro" sort="Nishidate, Kentaro" uniqKey="Nishidate K" first="Kentaro" last="Nishidate">Kentaro Nishidate</name>
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<author><name sortKey="Saito, Yuta" sort="Saito, Yuta" uniqKey="Saito Y" first="Yuta" last="Saito">Yuta Saito</name>
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<author><name sortKey="Mori, Kunihiko" sort="Mori, Kunihiko" uniqKey="Mori K" first="Kunihiko" last="Mori">Kunihiko Mori</name>
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<author><name sortKey="Asakawa, Daiki" sort="Asakawa, Daiki" uniqKey="Asakawa D" first="Daiki" last="Asakawa">Daiki Asakawa</name>
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<author><name sortKey="Takeda, Sen" sort="Takeda, Sen" uniqKey="Takeda S" first="Sen" last="Takeda">Sen Takeda</name>
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<author><name sortKey="Kubota, Takeo" sort="Kubota, Takeo" uniqKey="Kubota T" first="Takeo" last="Kubota">Takeo Kubota</name>
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<author><name sortKey="Terada, Nobuo" sort="Terada, Nobuo" uniqKey="Terada N" first="Nobuo" last="Terada">Nobuo Terada</name>
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<author><name sortKey="Hashimoto, Yutaka" sort="Hashimoto, Yutaka" uniqKey="Hashimoto Y" first="Yutaka" last="Hashimoto">Yutaka Hashimoto</name>
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<author><name sortKey="Hori, Hirokazu" sort="Hori, Hirokazu" uniqKey="Hori H" first="Hirokazu" last="Hori">Hirokazu Hori</name>
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<author><name sortKey="Hiraoka, Kenzo" sort="Hiraoka, Kenzo" uniqKey="Hiraoka K" first="Kenzo" last="Hiraoka">Kenzo Hiraoka</name>
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<term>Breast Feeding</term>
<term>Cattle</term>
<term>Citrus sinensis (chemistry)</term>
<term>Eggs (analysis)</term>
<term>Female</term>
<term>Gramicidin (analysis)</term>
<term>Humans</term>
<term>Insulin (analysis)</term>
<term>Liver (chemistry)</term>
<term>Mice</term>
<term>Milk (chemistry)</term>
<term>Milk, Human (chemistry)</term>
<term>Musa (chemistry)</term>
<term>Salmon (anatomy & histology)</term>
<term>Sensitivity and Specificity</term>
<term>Spectrometry, Mass, Electrospray Ionization (instrumentation)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
<term>Urine (chemistry)</term>
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<term>Insulin</term>
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<keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Salmon</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Liver</term>
<term>Milk</term>
<term>Milk, Human</term>
<term>Musa</term>
<term>Urine</term>
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<term>Spectrometry, Mass, Electrospray Ionization</term>
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<term>Brain Chemistry</term>
<term>Breast Feeding</term>
<term>Cattle</term>
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<term>Humans</term>
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<front><div type="abstract" xml:lang="en">Recently, we have developed probe electrospray ionization (PESI) that uses a solid needle. In this system, the probe needle moves up and down along the vertical axis by a motor-driven system. At the highest position of the probe needle, electrospray is generated by applying a high voltage. In this study, we applied PESI directly to biological samples such as urine, mouse brain, mouse liver, salmon egg, and fruits (orange, banana, etc.). Strong ion signals for almost all the samples were obtained. The amount of liquid sample picked up by the needle is as small as pL or less, making PESI a promising non-invasive technique for detecting biomolecules in living systems such as cells. Therefore, PESI may be useful as a versatile and ready-to-use semi-online analytical tool in the fields of medicine, pharmaceuticals, agriculture, food science, etc.</div>
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