Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.
Identifieur interne : 001D70 ( Main/Merge ); précédent : 001D69; suivant : 001D71Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.
Auteurs : Jeong-Soon Kim [États-Unis] ; Uma Shankar Sagaram ; Jacqueline K. Burns ; Jian-Liang Li ; Nian WangSource :
- Phytopathology [ 0031-949X ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Plant Proteins.
- immunology : Gene Expression Regulation, Plant, Plant Diseases.
- microbiology : Citrus, Plant Diseases, Plant Leaves.
- physiology : Rhizobiaceae.
- Expressed Sequence Tags, Protein Array Analysis.
Abstract
Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'Candidatus Liberibacter spp.' In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with citrus microarray hybridization based on 33,879 expressed sequence tag sequences from several citrus species and hybrids. The microarray analysis indicated that HLB infection significantly affected expression of 624 genes whose encoded proteins were categorized according to function. The categories included genes associated with sugar metabolism, plant defense, phytohormone, and cell wall metabolism, as well as 14 other gene categories. The anatomical analyses indicated that HLB bacterium infection caused phloem disruption, sucrose accumulation, and plugged sieve pores. The up-regulation of three key starch biosynthetic genes including ADP-glucose pyrophosphorylase, starch synthase, granule-bound starch synthase and starch debranching enzyme likely contributed to accumulation of starch in HLB-affected leaves. The HLB-associated phloem blockage resulted from the plugged sieve pores rather than the HLB bacterial aggregates since 'Ca. Liberibacter asiaticus' does not form aggregate in citrus. The up-regulation of pp2 gene is related to callose deposition to plug the sieve pores in HLB-affected plants.
DOI: 10.1094/PHYTO-99-1-0050
PubMed: 19055434
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.</title><author><name sortKey="Kim, Jeong Soon" sort="Kim, Jeong Soon" uniqKey="Kim J" first="Jeong-Soon" last="Kim">Jeong-Soon Kim</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Sagaram, Uma Shankar" sort="Sagaram, Uma Shankar" uniqKey="Sagaram U" first="Uma Shankar" last="Sagaram">Uma Shankar Sagaram</name></author><author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K" last="Burns">Jacqueline K. Burns</name></author><author><name sortKey="Li, Jian Liang" sort="Li, Jian Liang" uniqKey="Li J" first="Jian-Liang" last="Li">Jian-Liang Li</name></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19055434</idno><idno type="pmid">19055434</idno><idno type="doi">10.1094/PHYTO-99-1-0050</idno><idno type="wicri:Area/PubMed/Corpus">000A05</idno><idno type="wicri:Area/PubMed/Curation">000A05</idno><idno type="wicri:Area/PubMed/Checkpoint">000A05</idno><idno type="wicri:Area/Ncbi/Merge">000925</idno><idno type="wicri:Area/Ncbi/Curation">000925</idno><idno type="wicri:Area/Ncbi/Checkpoint">000925</idno><idno type="wicri:doubleKey">0031-949X:2009:Kim J:response:of:sweet</idno><idno type="wicri:Area/Main/Merge">001D70</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.</title><author><name sortKey="Kim, Jeong Soon" sort="Kim, Jeong Soon" uniqKey="Kim J" first="Jeong-Soon" last="Kim">Jeong-Soon Kim</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Sagaram, Uma Shankar" sort="Sagaram, Uma Shankar" uniqKey="Sagaram U" first="Uma Shankar" last="Sagaram">Uma Shankar Sagaram</name></author><author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K" last="Burns">Jacqueline K. Burns</name></author><author><name sortKey="Li, Jian Liang" sort="Li, Jian Liang" uniqKey="Li J" first="Jian-Liang" last="Li">Jian-Liang Li</name></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (microbiology)</term><term>Expressed Sequence Tags</term><term>Gene Expression Regulation, Plant (immunology)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (metabolism)</term><term>Protein Array Analysis</term><term>Rhizobiaceae (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Rhizobiaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Expressed Sequence Tags</term><term>Protein Array Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'Candidatus Liberibacter spp.' In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with citrus microarray hybridization based on 33,879 expressed sequence tag sequences from several citrus species and hybrids. The microarray analysis indicated that HLB infection significantly affected expression of 624 genes whose encoded proteins were categorized according to function. The categories included genes associated with sugar metabolism, plant defense, phytohormone, and cell wall metabolism, as well as 14 other gene categories. The anatomical analyses indicated that HLB bacterium infection caused phloem disruption, sucrose accumulation, and plugged sieve pores. The up-regulation of three key starch biosynthetic genes including ADP-glucose pyrophosphorylase, starch synthase, granule-bound starch synthase and starch debranching enzyme likely contributed to accumulation of starch in HLB-affected leaves. The HLB-associated phloem blockage resulted from the plugged sieve pores rather than the HLB bacterial aggregates since 'Ca. Liberibacter asiaticus' does not form aggregate in citrus. The up-regulation of pp2 gene is related to callose deposition to plug the sieve pores in HLB-affected plants.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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