Antioxidant potential of hydro-methanolic extract of seed of Caesalpinia bonduc: An in vitro study
Identifieur interne : 001822 ( Main/Merge ); précédent : 001821; suivant : 001823Antioxidant potential of hydro-methanolic extract of seed of Caesalpinia bonduc: An in vitro study
Auteurs : Kishalay Jana [Inde] ; Kausik Chatterjee [Inde] ; Kazi Monjur Ali [Inde] ; Abhinandan Ghosh [Inde] ; Tushar Kanti Bera [Inde] ; Debidas Ghosh [Inde]Source :
- Journal of Advanced Pharmaceutical Technology & Research [ 2231-4040 ] ; 2011.
Abstract
It is well known that the over production of reactive oxygen species is harmful for living organisms and it damages major cellular constituents such as DNA, protein, and lipid. At present, searching of new plant sources having free radical scavenging activity is an important field of research in phytomedicine as natural products are safe and relatively low cost. In this respect, attention has been focused to evaluate the antioxidant potential of hydro-methanolic extract of seed of
Url:
DOI: 10.4103/2231-4040.90884
PubMed: 22247894
PubMed Central: 3255348
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antioxidant potential of hydro-methanolic extract of seed of <italic>Caesalpinia bonduc</italic>: An <italic>in vitro</italic> study</title><author><name sortKey="Jana, Kishalay" sort="Jana, Kishalay" uniqKey="Jana K" first="Kishalay" last="Jana">Kishalay Jana</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. 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Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation></author><author><name sortKey="Chatterjee, Kausik" sort="Chatterjee, Kausik" uniqKey="Chatterjee K" first="Kausik" last="Chatterjee">Kausik Chatterjee</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation></author><author><name sortKey="Ali, Kazi Monjur" sort="Ali, Kazi Monjur" uniqKey="Ali K" first="Kazi Monjur" last="Ali">Kazi Monjur Ali</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation></author><author><name sortKey="Ghosh, Abhinandan" sort="Ghosh, Abhinandan" uniqKey="Ghosh A" first="Abhinandan" last="Ghosh">Abhinandan Ghosh</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation></author><author><name sortKey="Bera, Tushar Kanti" sort="Bera, Tushar Kanti" uniqKey="Bera T" first="Tushar Kanti" last="Bera">Tushar Kanti Bera</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Ghosh, Debidas" sort="Ghosh, Debidas" uniqKey="Ghosh D" first="Debidas" last="Ghosh">Debidas Ghosh</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Bio-Medical Laboratory Science and Management (U.G.C. Innovative Programme) Andrology, Endocrinology and Molecular Medicine Laboratory, Vidyasagar University, Midnapore</wicri:regionArea><wicri:noRegion>Midnapore</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Advanced Pharmaceutical Technology & Research</title><idno type="ISSN">2231-4040</idno><idno type="eISSN">0976-2094</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>It is well known that the over production of reactive oxygen species is harmful for living organisms and it damages major cellular constituents such as DNA, protein, and lipid. At present, searching of new plant sources having free radical scavenging activity is an important field of research in phytomedicine as natural products are safe and relatively low cost. In this respect, attention has been focused to evaluate the antioxidant potential of hydro-methanolic extract of seed of <italic>Caesalpinia bonduc</italic> (Caesalpenacae) using different <italic>in vitro</italic> models. To evaluate the antioxidant activity, extract was examined on 2, 2-diphenyl-1-picrylhydrazyl radical scavenging effect, scavenging of hydrogen peroxide, hydroxyl radical scavenging potential, and anti-lipid peroxidation activity by biochemical methods. Total phenol and flavonoids contents in the said extract were measured biochemically as per standard methods. Results were compared with butylated hydroxyl toluene and α-tocopherol. Results indicated that hydro-methanolic extract has strong scavenging activity on 2, 2-diphenyl-1-picrylhydrazyl radical with IC<sub>50</sub> value 157.4 μg/ml, hydroxyl radical with IC<sub>50</sub> value 61.9 μg/ml and hydrogen peroxide with IC<sub>50</sub> value 64.32 μg/ml. Hydro-methanolic extract also showed notable inhibition in lipid peroxidation having IC<sub>50</sub> value 58.87 μg/ml. Phytochemical study focused that the extract is rich in phenolic compounds (24.66 mg gallic acid equivalent/g dried extract) and flavonoids (136.65 mg quercetin equivalent/g dried extract). Findings of the experiment indicated that the hydro-methanolic extract of seed of <italic>Caesalpinia bonduc</italic> is a source of natural antioxidants.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Van De Vijver, Lp" uniqKey="Van De Vijver L">LP Van de Vijver</name></author><author><name sortKey="Kardinaal, Af" uniqKey="Kardinaal A">AF Kardinaal</name></author><author><name sortKey="Grobbee, De" uniqKey="Grobbee D">DE Grobbee</name></author><author><name sortKey="Princen, Hm" uniqKey="Princen H">HM Princen</name></author><author><name sortKey="Van Poppel, G" uniqKey="Van Poppel G">G Van Poppel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, Ma" uniqKey="Smith M">MA Smith</name></author><author><name sortKey="Perry, G" uniqKey="Perry G">G Perry</name></author><author><name sortKey="Pryor, Wa" uniqKey="Pryor W">WA 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H">H Kappus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ali, Km" uniqKey="Ali K">KM Ali</name></author><author><name sortKey="Chatterjee, K" uniqKey="Chatterjee K">K Chatterjee</name></author><author><name sortKey="De, D" uniqKey="De D">D De</name></author><author><name sortKey="Bera, Tk" uniqKey="Bera T">TK Bera</name></author><author><name sortKey="Mallick, C" uniqKey="Mallick C">C Mallick</name></author><author><name sortKey="Ghosh, D" uniqKey="Ghosh D">D Ghosh</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Antioxidant potential of hydro-methanolic extract of seed of Caesalpinia bonduc: An in vitro study
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