Comparison of different washing treatments for reducing pathogens on orange surfaces and for preventing the transfer of bacterial pathogens to fresh-squeezed orange juice.
Identifieur interne : 001794 ( Main/Merge ); précédent : 001793; suivant : 001795Comparison of different washing treatments for reducing pathogens on orange surfaces and for preventing the transfer of bacterial pathogens to fresh-squeezed orange juice.
Auteurs : N E Martínez-Gonzáles [Mexique] ; L. Martínez-Chávez ; C. Martínez-Cárdenas ; A. CastilloSource :
- Journal of food protection [ 1944-9097 ] ; 2011.
English descriptors
- KwdEn :
- Beverages (microbiology), Citrus sinensis (microbiology), Colony Count, Microbial, Consumer Product Safety, Dose-Response Relationship, Drug, Escherichia coli O157 (drug effects), Escherichia coli O157 (growth & development), Ethanol (pharmacology), Food Contamination (analysis), Food Contamination (prevention & control), Food Handling (methods), Food Microbiology, Humans, Hypochlorous Acid (pharmacology), Lactates (pharmacology), Listeria monocytogenes (drug effects), Listeria monocytogenes (growth & development), Salmonella (drug effects), Salmonella (growth & development), Temperature, Time Factors, Water (pharmacology).
- MESH :
- chemical , pharmacology : Ethanol, Hypochlorous Acid, Lactates, Water.
- analysis : Food Contamination.
- drug effects : Escherichia coli O157, Listeria monocytogenes, Salmonella.
- growth & development : Escherichia coli O157, Listeria monocytogenes, Salmonella.
- methods : Food Handling.
- microbiology : Beverages, Citrus sinensis.
- prevention & control : Food Contamination.
- Colony Count, Microbial, Consumer Product Safety, Dose-Response Relationship, Drug, Food Microbiology, Humans, Temperature, Time Factors.
Abstract
The objectives of this study were to compare the effectiveness of various washing treatments for reducing Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes populations on orange surfaces and to measure the effect of some of these treatments in preventing the transfer of pathogens during juice extraction. Orange surfaces inoculated with L. monocytogenes or a mixture of E. coli O157:H7 and Salmonella Typhimurium were washed by water spray and then sprayed with or dipped in water at 80°C for 1 min, 70% ethanol for 15, 30, or 45 s or 1, 2, or 4 min, 2 or 4% lactic acid solution at 55°C for 15, 30, or 45 s or 1, 2, or 4 min, or 200 mg/liter hypochlorite at pH 6.5 or 10 for 15 s. The surviving populations of these pathogens on the oranges were enumerated after each treatment. In a further stage, the ability of these pathogens to be transferred to the juice during extraction was tested. Juice was obtained from inoculated oranges that were subjected to selected treatments using chlorine, lactic acid, ethanol, and hot water as described above, and then bacterial counts in orange juice were determined. The application of these treatments reduced the populations of pathogens on orange surfaces by 1.9 to >4.9 log, 1.9 to >4.6 log, and 1.4 to 3.1 log cycles for E. coli O157:H7, Salmonella Typhimurium, and L. monocytogenes, respectively. The treatments using hot water or lactic acid showed greater reductions than other treatments. The time, antimicrobial concentration, and form of application affected the bacterial reduction. All treatments resulted in undetectable counts in the juice. Nevertheless, pathogens were recovered by the enrichment-plating method. Treatment of oranges before juice extraction may reduce the risk associated with consuming orange juice.
DOI: 10.4315/0362-028X.JFP-10-357
PubMed: 22004816
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pubmed:22004816Le document en format XML
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Martínez-Chávez</name></author><author><name sortKey="Martinez Cardenas, C" sort="Martinez Cardenas, C" uniqKey="Martinez Cardenas C" first="C" last="Martínez-Cárdenas">C. Martínez-Cárdenas</name></author><author><name sortKey="Castillo, A" sort="Castillo, A" uniqKey="Castillo A" first="A" last="Castillo">A. Castillo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:22004816</idno><idno type="pmid">22004816</idno><idno type="doi">10.4315/0362-028X.JFP-10-357</idno><idno type="wicri:Area/PubMed/Corpus">000699</idno><idno type="wicri:Area/PubMed/Curation">000699</idno><idno type="wicri:Area/PubMed/Checkpoint">000699</idno><idno type="wicri:Area/Ncbi/Merge">000E32</idno><idno type="wicri:Area/Ncbi/Curation">000E32</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E32</idno><idno type="wicri:Area/Main/Merge">001794</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of different washing treatments for reducing pathogens on orange surfaces and for preventing the transfer of bacterial pathogens to fresh-squeezed orange juice.</title><author><name sortKey="Martinez Gonzales, N E" sort="Martinez Gonzales, N E" uniqKey="Martinez Gonzales N" first="N E" last="Martínez-Gonzáles">N E Martínez-Gonzáles</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, México.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430</wicri:regionArea><wicri:noRegion>Jalisco 44430</wicri:noRegion></affiliation></author><author><name sortKey="Martinez Chavez, L" sort="Martinez Chavez, L" uniqKey="Martinez Chavez L" first="L" last="Martínez-Chávez">L. Martínez-Chávez</name></author><author><name sortKey="Martinez Cardenas, C" sort="Martinez Cardenas, C" uniqKey="Martinez Cardenas C" first="C" last="Martínez-Cárdenas">C. Martínez-Cárdenas</name></author><author><name sortKey="Castillo, A" sort="Castillo, A" uniqKey="Castillo A" first="A" last="Castillo">A. Castillo</name></author></analytic><series><title level="j">Journal of food protection</title><idno type="eISSN">1944-9097</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beverages (microbiology)</term><term>Citrus sinensis (microbiology)</term><term>Colony Count, Microbial</term><term>Consumer Product Safety</term><term>Dose-Response Relationship, Drug</term><term>Escherichia coli O157 (drug effects)</term><term>Escherichia coli O157 (growth & development)</term><term>Ethanol (pharmacology)</term><term>Food Contamination (analysis)</term><term>Food Contamination (prevention & control)</term><term>Food Handling (methods)</term><term>Food Microbiology</term><term>Humans</term><term>Hypochlorous Acid (pharmacology)</term><term>Lactates (pharmacology)</term><term>Listeria monocytogenes (drug effects)</term><term>Listeria monocytogenes (growth & development)</term><term>Salmonella (drug effects)</term><term>Salmonella (growth & development)</term><term>Temperature</term><term>Time Factors</term><term>Water (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Ethanol</term><term>Hypochlorous Acid</term><term>Lactates</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli O157</term><term>Listeria monocytogenes</term><term>Salmonella</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Escherichia coli O157</term><term>Listeria monocytogenes</term><term>Salmonella</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Handling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Beverages</term><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Food Contamination</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Colony Count, Microbial</term><term>Consumer Product Safety</term><term>Dose-Response Relationship, Drug</term><term>Food Microbiology</term><term>Humans</term><term>Temperature</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objectives of this study were to compare the effectiveness of various washing treatments for reducing Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes populations on orange surfaces and to measure the effect of some of these treatments in preventing the transfer of pathogens during juice extraction. Orange surfaces inoculated with L. monocytogenes or a mixture of E. coli O157:H7 and Salmonella Typhimurium were washed by water spray and then sprayed with or dipped in water at 80°C for 1 min, 70% ethanol for 15, 30, or 45 s or 1, 2, or 4 min, 2 or 4% lactic acid solution at 55°C for 15, 30, or 45 s or 1, 2, or 4 min, or 200 mg/liter hypochlorite at pH 6.5 or 10 for 15 s. The surviving populations of these pathogens on the oranges were enumerated after each treatment. In a further stage, the ability of these pathogens to be transferred to the juice during extraction was tested. Juice was obtained from inoculated oranges that were subjected to selected treatments using chlorine, lactic acid, ethanol, and hot water as described above, and then bacterial counts in orange juice were determined. The application of these treatments reduced the populations of pathogens on orange surfaces by 1.9 to >4.9 log, 1.9 to >4.6 log, and 1.4 to 3.1 log cycles for E. coli O157:H7, Salmonella Typhimurium, and L. monocytogenes, respectively. The treatments using hot water or lactic acid showed greater reductions than other treatments. The time, antimicrobial concentration, and form of application affected the bacterial reduction. All treatments resulted in undetectable counts in the juice. Nevertheless, pathogens were recovered by the enrichment-plating method. Treatment of oranges before juice extraction may reduce the risk associated with consuming orange juice.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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