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Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers

Identifieur interne : 001267 ( Main/Merge ); précédent : 001266; suivant : 001268

Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers

Auteurs : Komal Goswami ; R. Sharma ; P. K. Singh ; Govind Singh

Source :

Physiology and molecular biology of plants : an international journal of functional plant biology [ 0971-5894 ] ; 2012.

RBID : PMC:3550687

Abstract

A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l⁻¹) or kinetin (0.5 mg l⁻¹). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1 mg l⁻¹ BA and 0.5 mg l⁻¹ kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1 mg l⁻¹ each), it increased with increasing concentration of BA up to 10.0 mg l⁻¹. None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0 mg l⁻¹ BA + 0.5 mg l⁻¹ kinetin + 0.5 mg l⁻¹ IBA or 0.25 mg l⁻¹ BA + 1.0 mg l⁻¹ kinetin + 1.0 mg l⁻¹ IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0 mg l⁻¹ IBA. It is concluded that a five-fold increase (1.0 mg l⁻¹ BA + 0.5 mg l⁻¹ kinetin + 0.5 mg l⁻¹ IBA) in axillary shoot proliferation, while seven-fold increase (0.25 mg/l mg l⁻¹ BA + 1.0 mg l⁻¹ kinetin + 1.0 mg l⁻¹ IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3550687

DOI: 10.1007/s12298-012-0148-0
PubMed: 24381445
PubMed Central: 3550687

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PMC:3550687

Le document en format XML

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 L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers</title>
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<author><name sortKey="Sharma, R" sort="Sharma, R" uniqKey="Sharma R" first="R." last="Sharma">R. Sharma</name>
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<author><name sortKey="Singh, P K" sort="Singh, P K" uniqKey="Singh P" first="P. K." last="Singh">P. K. Singh</name>
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<affiliation><nlm:aff id="Aff2">IIVR-Seed Production Centre, Sargatia, Seorahi, Kushinagar, U.P. India</nlm:aff>
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<author><name sortKey="Singh, Govind" sort="Singh, Govind" uniqKey="Singh G" first="Govind" last="Singh">Govind Singh</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Micropropagation of seedless lemon (<italic>Citrus limon</italic>
 L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers</title>
<author><name sortKey="Goswami, Komal" sort="Goswami, Komal" uniqKey="Goswami K" first="Komal" last="Goswami">Komal Goswami</name>
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<author><name sortKey="Singh, P K" sort="Singh, P K" uniqKey="Singh P" first="P. K." last="Singh">P. K. Singh</name>
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<wicri:noCountry code="subfield">334006 Rajasthan India</wicri:noCountry>
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<affiliation><nlm:aff id="Aff2">IIVR-Seed Production Centre, Sargatia, Seorahi, Kushinagar, U.P. India</nlm:aff>
<wicri:noCountry code="subfield">U.P. India</wicri:noCountry>
</affiliation>
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<author><name sortKey="Singh, Govind" sort="Singh, Govind" uniqKey="Singh G" first="Govind" last="Singh">Govind Singh</name>
<affiliation><nlm:aff id="Aff1">Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, Bikaner, 334006 Rajasthan India</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p>A micropropagation protocol was developed for multiplication of seedless lemon (<italic>Citrus limon</italic>
 L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l<sup>−1</sup>
) or kinetin (0.5 mg l<sup>−1</sup>
). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1 mg l<sup>−1</sup>
 BA and 0.5 mg l<sup>−1</sup>
 kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1 mg l<sup>−1</sup>
 each), it increased with increasing concentration of BA up to 10.0 mg l<sup>−1</sup>
. None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0 mg l<sup>−1</sup>
 BA + 0.5 mg l<sup>−1</sup>
 kinetin + 0.5 mg l<sup>−1</sup>
 IBA or 0.25 mg l<sup>−1</sup>
 BA + 1.0 mg l<sup>−1</sup>
 kinetin + 1.0 mg l<sup>−1</sup>
 IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0 mg l<sup>−1</sup>
 IBA. It is concluded that a five-fold increase (1.0 mg l<sup>−1</sup>
 BA + 0.5 mg l<sup>−1</sup>
 kinetin + 0.5 mg l<sup>−1</sup>
 IBA) in axillary shoot proliferation, while seven-fold increase (0.25 mg/l mg l<sup>−1</sup>
 BA + 1.0 mg l<sup>−1</sup>
 kinetin + 1.0 mg l<sup>−1</sup>
 IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.</p>
</div>
</front>
</TEI>
</record>

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Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers

Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers

Source :

Abstract

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