Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a TLC-densitometric method
Identifieur interne : 000449 ( Main/Merge ); précédent : 000448; suivant : 000450Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a TLC-densitometric method
Auteurs : Ammar Altemimi [États-Unis, Iraq] ; Dennis G. Watson [États-Unis] ; Mary Kinsel [États-Unis] ; David A. Lightfoot [États-Unis]Source :
- Chemistry Central Journal [ 1752-153X ] ; 2015.
Abstract
The use of medicinal plants has been reported throughout human history. In the fight against illnesses, medicinal plants represent the primary health care system for 60 % of the world’s population. Flavonoids are polyphenolic compounds with active anti-microbial properties; they are produced in plants as pigments. Quercetin, myricetin, and rutin are among the most well-known and prevalent flavonoids in plants, with an antioxidant activity capable of decreasing the oxidation of low density lipoproteins [LDLs]. To date, this research is the first of its kind to employ a coupled thin-layer chromatography (TLC) and a densitometric quantification method with a Box-Behnken design (BBD) response surface methodology (RSM) for optimization of ultrasonic-assisted extraction and determination of rutin and quercetin from peach and ellagic acid and myricetin from pumpkin fruits.
The effect of process variables (extraction temperature (°C), extraction power (%) and extraction time (min)) on ultrasound-assisted extraction (UAE) were examined by using BBD and RSM. TLC followed by Quantity-One™ (BioRad) image analysis as a simple and rapid method was used for identification and quantification of the compounds in complex mixtures. The results were consistent under optimal conditions among the experimental values and their predicted values. A mass spectrometry (MALDI-TOF MS) technique was also used to confirm the identity of the natural products in the TLC spots resolved.
The results show that the coupled TLC-densitometric methods & BBD can be a very powerful approach to qualitative and quantitative analysis of; rutin and quercetin from peach extracts; and ellagic acid and myricetin contents from pumpkin extracts.
Url:
DOI: 10.1186/s13065-015-0113-4
PubMed: 26106445
PubMed Central: 4477078
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Watson</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale</wicri:cityArea></affiliation></author><author><name sortKey="Kinsel, Mary" sort="Kinsel, Mary" uniqKey="Kinsel M" first="Mary" last="Kinsel">Mary Kinsel</name><affiliation wicri:level="2"><nlm:aff id="Aff3">SIUC Mass Spectrometry Facility, Department of Chemistry and Biochemistry, SIUC, Carbondale, IL 62901 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>SIUC Mass Spectrometry Facility, Department of Chemistry and Biochemistry, SIUC, Carbondale</wicri:cityArea></affiliation></author><author><name sortKey="Lightfoot, David A" sort="Lightfoot, David A" uniqKey="Lightfoot D" first="David A." last="Lightfoot">David A. 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Watson</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale</wicri:cityArea></affiliation></author><author><name sortKey="Kinsel, Mary" sort="Kinsel, Mary" uniqKey="Kinsel M" first="Mary" last="Kinsel">Mary Kinsel</name><affiliation wicri:level="2"><nlm:aff id="Aff3">SIUC Mass Spectrometry Facility, Department of Chemistry and Biochemistry, SIUC, Carbondale, IL 62901 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>SIUC Mass Spectrometry Facility, Department of Chemistry and Biochemistry, SIUC, Carbondale</wicri:cityArea></affiliation></author><author><name sortKey="Lightfoot, David A" sort="Lightfoot, David A" uniqKey="Lightfoot D" first="David A." last="Lightfoot">David A. Lightfoot</name><affiliation wicri:level="2"><nlm:aff id="Aff1">Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale</wicri:cityArea></affiliation></author></analytic><series><title level="j">Chemistry Central Journal</title><idno type="eISSN">1752-153X</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The use of medicinal plants has been reported throughout human history. In the fight against illnesses, medicinal plants represent the primary health care system for 60 % of the world’s population. Flavonoids are polyphenolic compounds with active anti-microbial properties; they are produced in plants as pigments. Quercetin, myricetin, and rutin are among the most well-known and prevalent flavonoids in plants, with an antioxidant activity capable of decreasing the oxidation of low density lipoproteins [LDLs]. To date, this research is the first of its kind to employ a coupled thin-layer chromatography (TLC) and a densitometric quantification method with a Box-Behnken design (BBD) response surface methodology (RSM) for optimization of ultrasonic-assisted extraction and determination of rutin and quercetin from peach and ellagic acid and myricetin from pumpkin fruits.</p></sec><sec><title>Results</title><p>The effect of process variables (extraction temperature (°C), extraction power (%) and extraction time (min)) on ultrasound-assisted extraction (UAE) were examined by using BBD and RSM. TLC followed by Quantity-One™ (BioRad) image analysis as a simple and rapid method was used for identification and quantification of the compounds in complex mixtures. The results were consistent under optimal conditions among the experimental values and their predicted values. A mass spectrometry (MALDI-TOF MS) technique was also used to confirm the identity of the natural products in the TLC spots resolved.</p></sec><sec><title>Conclusion</title><p>The results show that the coupled TLC-densitometric methods & BBD can be a very powerful approach to qualitative and quantitative analysis of; rutin and quercetin from peach extracts; and ellagic acid and myricetin contents from pumpkin extracts.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Brusotti, G" uniqKey="Brusotti G">G Brusotti</name></author><author><name sortKey="Cesari, I" uniqKey="Cesari I">I Cesari</name></author><author><name sortKey="Dentamaro, A" uniqKey="Dentamaro A">A Dentamaro</name></author><author><name sortKey="Caccialanza, G" uniqKey="Caccialanza G">G Caccialanza</name></author><author><name sortKey="Massolini, G" uniqKey="Massolini G">G Massolini</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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|clé= PMC:4477078
|texte= Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a TLC-densitometric method
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:26106445" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |