Genome-wide identification and expression analysis of the polyamine oxidase gene family in sweet orange (Citrus sinensis).
Identifieur interne : 000621 ( Main/Exploration ); précédent : 000620; suivant : 000622Genome-wide identification and expression analysis of the polyamine oxidase gene family in sweet orange (Citrus sinensis).
Auteurs : Wei Wang [République populaire de Chine] ; Ji-Hong Liu [République populaire de Chine]Source :
- Gene [ 1879-0038 ] ; 2015.
English descriptors
- KwdEn :
- Amino Acid Sequence, Citrus sinensis (enzymology), Citrus sinensis (genetics), Cotyledon (metabolism), Gene Expression Profiling, Gene Expression Regulation, Plant, Genetic Association Studies, Genetic Variation, Molecular Sequence Data, Multigene Family, Oxidoreductases Acting on CH-NH Group Donors (genetics), Oxidoreductases Acting on CH-NH Group Donors (metabolism), Phylogeny, Plant Leaves (metabolism), Plant Roots (metabolism), Plant Stems (metabolism), Polyamines (metabolism), Promoter Regions, Genetic, Sequence Homology, Amino Acid.
- MESH :
- chemical , genetics : Oxidoreductases Acting on CH-NH Group Donors.
- enzymology : Citrus sinensis.
- genetics : Citrus sinensis.
- metabolism : Cotyledon, Oxidoreductases Acting on CH-NH Group Donors, Plant Leaves, Plant Roots, Plant Stems, Polyamines.
- Amino Acid Sequence, Gene Expression Profiling, Gene Expression Regulation, Plant, Genetic Association Studies, Genetic Variation, Molecular Sequence Data, Multigene Family, Phylogeny, Promoter Regions, Genetic, Sequence Homology, Amino Acid.
Abstract
Polyamine oxidases (PAOs) are FAD-dependent enzymes associated with polyamine catabolism. In plants, increasing evidences support that PAO genes play essential roles in abiotic and biotic stresses response. In this study, six putative PAO genes (CsPAO1-CsPAO6) were unraveled in sweet orange (Citrus sinensis) using the released citrus genome sequences. A total of 203 putative cis-regulatory elements involved in hormone and stress response were predicted in 1.5-kb promoter regions at the upstream of CsPAOs. The CsPAOs can be divided into four major groups, with similar organizations with their counterparts of Arabidopsis thaliana. Transcripts of CsPAOs were detected in leaf, stem, cotyledon, and root, with the highest levels detected in the roots. The CsPAOs displayed various responses to exogenous treatments with polyamines and ABA and were differentially altered by abiotic stresses, including cold, salt, and mannitol. Overexpression of CsPAO3 in tobacco demonstrated that spermidine and spermine were decreased in the transgenic line, while putrescine was significantly enhanced, implying a potential role of this gene in polyamine back conversion. These data provide valuable knowledge for understanding the roles of the PAO genes in the future.
DOI: 10.1016/j.gene.2014.11.042
PubMed: 25445392
Affiliations:
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Electronic address: liujihong@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25445392</idno><idno type="pmid">25445392</idno><idno type="doi">10.1016/j.gene.2014.11.042</idno><idno type="wicri:Area/PubMed/Corpus">000232</idno><idno type="wicri:Area/PubMed/Curation">000232</idno><idno type="wicri:Area/PubMed/Checkpoint">000232</idno><idno type="wicri:Area/Ncbi/Merge">001924</idno><idno type="wicri:Area/Ncbi/Curation">001924</idno><idno type="wicri:Area/Ncbi/Checkpoint">001924</idno><idno type="wicri:Area/Main/Merge">000621</idno><idno type="wicri:Area/Main/Curation">000621</idno><idno type="wicri:Area/Main/Exploration">000621</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genome-wide identification and expression analysis of the polyamine oxidase gene family in sweet orange (Citrus sinensis).</title><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: liujihong@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Citrus sinensis (enzymology)</term><term>Citrus sinensis (genetics)</term><term>Cotyledon (metabolism)</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genetic Association Studies</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Multigene Family</term><term>Oxidoreductases Acting on CH-NH Group Donors (genetics)</term><term>Oxidoreductases Acting on CH-NH Group Donors (metabolism)</term><term>Phylogeny</term><term>Plant Leaves (metabolism)</term><term>Plant Roots (metabolism)</term><term>Plant Stems (metabolism)</term><term>Polyamines (metabolism)</term><term>Promoter Regions, Genetic</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oxidoreductases Acting on CH-NH Group Donors</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cotyledon</term><term>Oxidoreductases Acting on CH-NH Group Donors</term><term>Plant Leaves</term><term>Plant Roots</term><term>Plant Stems</term><term>Polyamines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genetic Association Studies</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Multigene Family</term><term>Phylogeny</term><term>Promoter Regions, Genetic</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polyamine oxidases (PAOs) are FAD-dependent enzymes associated with polyamine catabolism. In plants, increasing evidences support that PAO genes play essential roles in abiotic and biotic stresses response. In this study, six putative PAO genes (CsPAO1-CsPAO6) were unraveled in sweet orange (Citrus sinensis) using the released citrus genome sequences. A total of 203 putative cis-regulatory elements involved in hormone and stress response were predicted in 1.5-kb promoter regions at the upstream of CsPAOs. The CsPAOs can be divided into four major groups, with similar organizations with their counterparts of Arabidopsis thaliana. Transcripts of CsPAOs were detected in leaf, stem, cotyledon, and root, with the highest levels detected in the roots. The CsPAOs displayed various responses to exogenous treatments with polyamines and ABA and were differentially altered by abiotic stresses, including cold, salt, and mannitol. Overexpression of CsPAO3 in tobacco demonstrated that spermidine and spermine were decreased in the transgenic line, while putrescine was significantly enhanced, implying a potential role of this gene in polyamine back conversion. These data provide valuable knowledge for understanding the roles of the PAO genes in the future.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></noRegion><name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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