Orange juice and hesperidin promote differential innate immune response in macrophages ex vivo.
Identifieur interne : 000D66 ( Main/Exploration ); précédent : 000D65; suivant : 000D67Orange juice and hesperidin promote differential innate immune response in macrophages ex vivo.
Auteurs : Grace Kelly Zanotti Simoes Dourado [Brésil] ; Lívia C. De Abreu Ribeiro [Brésil] ; Iracilda Zeppone Carlos [Brésil] ; Thais Borges César [Brésil]Source :
- International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition [ 0300-9831 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Beverages, Cells, Cultured, Citrus sinensis, Cytokines (secretion), Fruit, Hesperidin (pharmacology), Interleukin-10 (analysis), Interleukin-12 (analysis), Lipopolysaccharides (pharmacology), Macrophages (drug effects), Macrophages (immunology), Mice, Nitric Oxide (analysis), Nitric Oxide (secretion), Peritoneal Cavity (cytology), Peritoneal Lavage, Tumor Necrosis Factor-alpha (analysis).
- MESH :
- chemical , analysis : Interleukin-10, Interleukin-12, Nitric Oxide, Tumor Necrosis Factor-alpha.
- chemical , pharmacology : Hesperidin, Lipopolysaccharides.
- chemical , secretion : Cytokines, Nitric Oxide.
- cytology : Peritoneal Cavity.
- drug effects : Macrophages.
- immunology : Macrophages.
- Animals, Beverages, Cells, Cultured, Citrus sinensis, Fruit, Mice, Peritoneal Lavage.
Abstract
The purpose of this study was to verify the immune response induced by intake of orange juice or hesperidin on macrophages through the secretion of cytokines and nitric oxide. Mice were divided in three groups treated orally with orange juice, hesperidin, or control for 2 weeks. Ex vivo macrophages from all groups of mice were cultured with or without lipopolysaccharide (LPS)-stimuli, and the levels of nitric oxide (NO) and of the cytokines IL-10, IL-12, and TNF-alpha were evaluated. Macrophages of non-LPS-stimulated, orange juice treatment increased IL-12 levels by 143 % and the other cytokines and NO levels were unchanged. Hesperidin treatment increased IL-12 levels by 72 % and strongly decreased the NO secretion. For LPS-stimulated macrophages the orange juice (OJ) treatment decreased TNF-alpha secretion by 100 % and did not alter other cytokines, while NO levels increased 41 %. Hesperidin treatment decreased NO, IL-10, IL-12, and TNF-alpha levels by 56 %, 47 %, 29 %, and 63 %, respectively. In conclusion, OJ and hesperidin showed different immune responses, suggesting that hesperidin displays a suppressive effect on inflammation generated by LPS, while OJ seems to enhance the functions of macrophages associated with antimicrobial activity.
DOI: 10.1024/0300-9831/a000157
PubMed: 24846905
Affiliations:
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Le document en format XML
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De Abreu Ribeiro</name><affiliation wicri:level="2"><nlm:affiliation>Department of Clinical Analysis, Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Clinical Analysis, Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Zeppone Carlos, Iracilda" sort="Zeppone Carlos, Iracilda" uniqKey="Zeppone Carlos I" first="Iracilda" last="Zeppone Carlos">Iracilda Zeppone Carlos</name><affiliation wicri:level="2"><nlm:affiliation>Department of Clinical Analysis, Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Clinical Analysis, Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Borges Cesar, Thais" sort="Borges Cesar, Thais" uniqKey="Borges Cesar T" first="Thais" last="Borges César">Thais Borges César</name><affiliation wicri:level="2"><nlm:affiliation>Department of Food and Nutrition, Laboratory of Nutrition, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Food and Nutrition, Laboratory of Nutrition, Faculty of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author></analytic><series><title level="j">International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition</title><idno type="ISSN">0300-9831</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Beverages</term><term>Cells, Cultured</term><term>Citrus sinensis</term><term>Cytokines (secretion)</term><term>Fruit</term><term>Hesperidin (pharmacology)</term><term>Interleukin-10 (analysis)</term><term>Interleukin-12 (analysis)</term><term>Lipopolysaccharides (pharmacology)</term><term>Macrophages (drug effects)</term><term>Macrophages (immunology)</term><term>Mice</term><term>Nitric Oxide (analysis)</term><term>Nitric Oxide (secretion)</term><term>Peritoneal Cavity (cytology)</term><term>Peritoneal Lavage</term><term>Tumor Necrosis Factor-alpha (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Interleukin-10</term><term>Interleukin-12</term><term>Nitric Oxide</term><term>Tumor Necrosis Factor-alpha</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Hesperidin</term><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="secretion" xml:lang="en"><term>Cytokines</term><term>Nitric Oxide</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Peritoneal Cavity</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Macrophages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Beverages</term><term>Cells, Cultured</term><term>Citrus sinensis</term><term>Fruit</term><term>Mice</term><term>Peritoneal Lavage</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to verify the immune response induced by intake of orange juice or hesperidin on macrophages through the secretion of cytokines and nitric oxide. Mice were divided in three groups treated orally with orange juice, hesperidin, or control for 2 weeks. Ex vivo macrophages from all groups of mice were cultured with or without lipopolysaccharide (LPS)-stimuli, and the levels of nitric oxide (NO) and of the cytokines IL-10, IL-12, and TNF-alpha were evaluated. Macrophages of non-LPS-stimulated, orange juice treatment increased IL-12 levels by 143 % and the other cytokines and NO levels were unchanged. Hesperidin treatment increased IL-12 levels by 72 % and strongly decreased the NO secretion. For LPS-stimulated macrophages the orange juice (OJ) treatment decreased TNF-alpha secretion by 100 % and did not alter other cytokines, while NO levels increased 41 %. Hesperidin treatment decreased NO, IL-10, IL-12, and TNF-alpha levels by 56 %, 47 %, 29 %, and 63 %, respectively. In conclusion, OJ and hesperidin showed different immune responses, suggesting that hesperidin displays a suppressive effect on inflammation generated by LPS, while OJ seems to enhance the functions of macrophages associated with antimicrobial activity.</div></front></TEI><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Zanotti Simoes Dourado, Grace Kelly" sort="Zanotti Simoes Dourado, Grace Kelly" uniqKey="Zanotti Simoes Dourado G" first="Grace Kelly" last="Zanotti Simoes Dourado">Grace Kelly Zanotti Simoes Dourado</name></region><name sortKey="Borges Cesar, Thais" sort="Borges Cesar, Thais" uniqKey="Borges Cesar T" first="Thais" last="Borges César">Thais Borges César</name><name sortKey="De Abreu Ribeiro, Livia C" sort="De Abreu Ribeiro, Livia C" uniqKey="De Abreu Ribeiro L" first="Lívia C" last="De Abreu Ribeiro">Lívia C. De Abreu Ribeiro</name><name sortKey="Zeppone Carlos, Iracilda" sort="Zeppone Carlos, Iracilda" uniqKey="Zeppone Carlos I" first="Iracilda" last="Zeppone Carlos">Iracilda Zeppone Carlos</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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