Comparative proteomics of a lycopene‐accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck)
Identifieur interne : 001F01 ( Main/Exploration ); précédent : 001F00; suivant : 001F02Comparative proteomics of a lycopene‐accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (Citrus sinensis [L.] osbeck)
Auteurs : Zhiyong Pan [République populaire de Chine] ; Qing Liu [République populaire de Chine] ; Ze Yun [République populaire de Chine] ; Rui Guan [République populaire de Chine] ; Wenfang Zeng [République populaire de Chine] ; Qiang Xu [République populaire de Chine] ; Xiuxin Deng [République populaire de Chine]Source :
- PROTEOMICS [ 1615-9853 ] ; 2009-12.
English descriptors
- KwdEn :
- Antioxidants (metabolism), Carotenoids (metabolism), Citrus, Citrus sinensis (genetics), Citrus sinensis (metabolism), Fruit (drug effects), Fruit (metabolism), Gene Expression Regulation, Plant, Genes, Plant, Lycopene accumulation, Mutation, Oxidative Stress, Oxidative stress, Plant Proteins (analysis), Plant Proteins (metabolism), Plant proteomics, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transcription, Genetic, tert-Butylhydroperoxide (pharmacology).
- MESH :
- chemical , analysis : Plant Proteins.
- chemical , metabolism : Antioxidants, Carotenoids, Plant Proteins.
- drug effects : Fruit.
- genetics : Citrus sinensis.
- metabolism : Citrus sinensis, Fruit.
- chemical , pharmacology : tert-Butylhydroperoxide.
- Gene Expression Regulation, Plant, Genes, Plant, Mutation, Oxidative Stress, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transcription, Genetic.
Abstract
A spontaneous sweet orange (Citrus sinenesis [L.] Osbeck) mutant ‘Hong Anliu’ is of high value due to lycopene accumulation in the pulp. In this study, we analyzed the proteomic alterations in the pulp of ‘Hong Anliu’ versus its wild type (WT) at four maturing stages by using 2‐DE combined with MALDI‐TOF‐TOF MS. Among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, modification and degradation. Particularly, expression levels of six anti‐oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in ‘Hong Anliu’, implying a regulatory role of oxidative stress on carotenogenesis. This conclusion was further confirmed by our observation that treatment of fruit pulps with tert‐butylhydroperoxide (a ROS progenitor) induced lycopene accumulation in ‘Hong Anliu’ only. Gene expression showed that genes predicted to function upstream of lycopene biosynthesis were generally upregulated in juice sacs, but downregulated in segment membranes in both ‘Hong Anliu’ and its WT. The result suggests an important role of post‐transcriptional regulation on carotenogenesis since lycopene was induced in ‘Hong Anliu’ but not WT. The result also implies that carotenogenesis in juice sacs and segment membranes of citrus fruits may be regulated by different mechanisms.
Url:
DOI: 10.1002/pmic.200900092
Affiliations:
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ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A spontaneous sweet orange (Citrus sinenesis [L.] Osbeck) mutant ‘Hong Anliu’ is of high value due to lycopene accumulation in the pulp. In this study, we analyzed the proteomic alterations in the pulp of ‘Hong Anliu’ versus its wild type (WT) at four maturing stages by using 2‐DE combined with MALDI‐TOF‐TOF MS. Among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, modification and degradation. Particularly, expression levels of six anti‐oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in ‘Hong Anliu’, implying a regulatory role of oxidative stress on carotenogenesis. This conclusion was further confirmed by our observation that treatment of fruit pulps with tert‐butylhydroperoxide (a ROS progenitor) induced lycopene accumulation in ‘Hong Anliu’ only. Gene expression showed that genes predicted to function upstream of lycopene biosynthesis were generally upregulated in juice sacs, but downregulated in segment membranes in both ‘Hong Anliu’ and its WT. The result suggests an important role of post‐transcriptional regulation on carotenogenesis since lycopene was induced in ‘Hong Anliu’ but not WT. The result also implies that carotenogenesis in juice sacs and segment membranes of citrus fruits may be regulated by different mechanisms.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name></noRegion><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><name sortKey="Guan, Rui" sort="Guan, Rui" uniqKey="Guan R" first="Rui" last="Guan">Rui Guan</name><name sortKey="Liu, Qing" sort="Liu, Qing" uniqKey="Liu Q" first="Qing" last="Liu">Qing Liu</name><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><name sortKey="Yun, Ze" sort="Yun, Ze" uniqKey="Yun Z" first="Ze" last="Yun">Ze Yun</name><name sortKey="Zeng, Wenfang" sort="Zeng, Wenfang" uniqKey="Zeng W" first="Wenfang" last="Zeng">Wenfang Zeng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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