Involvement of polyamine biosynthesis in somatic embryogenesis of Valencia sweet orange (Citrus sinensis) induced by glycerol.
Identifieur interne : 001D53 ( Main/Exploration ); précédent : 001D52; suivant : 001D54Involvement of polyamine biosynthesis in somatic embryogenesis of Valencia sweet orange (Citrus sinensis) induced by glycerol.
Auteurs : Xiao-Ba Wu [République populaire de Chine] ; Jing Wang ; Ji-Hong Liu ; Xiu-Xin DengSource :
- Journal of plant physiology [ 1618-1328 ] ; 2009.
English descriptors
- KwdEn :
- Citrus sinensis (embryology), Citrus sinensis (genetics), Citrus sinensis (metabolism), Culture Media, DNA, Complementary (isolation & purification), Eflornithine (pharmacology), Embryonic Development (drug effects), Embryonic Development (genetics), Gene Expression Regulation, Plant (drug effects), Glycerol (pharmacology), Morphogenesis (drug effects), Plant Proteins (genetics), Plant Proteins (metabolism), Polyamines (metabolism), Putrescine (pharmacology), Seeds (drug effects), Seeds (genetics), Time Factors, Tissue Culture Techniques.
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , isolation & purification : DNA, Complementary.
- chemical , metabolism : Plant Proteins, Polyamines.
- chemical , pharmacology : Eflornithine, Glycerol, Putrescine.
- chemical : Culture Media.
- drug effects : Embryonic Development, Gene Expression Regulation, Plant, Morphogenesis, Seeds.
- embryology : Citrus sinensis.
- genetics : Citrus sinensis, Embryonic Development, Seeds.
- metabolism : Citrus sinensis.
- Time Factors, Tissue Culture Techniques.
Abstract
Culture of Citrus sinensis embryogenic callus on the embryo-inducing medium (EIM) containing glycerol gave rise to a large number of embryos, whereas very few embryos were observed on the callus growth medium (CGM). In the current paper, attempts were made to investigate whether polyamine biosynthesis was involved in glycerol-mediated somatic embryogenesis. Quantification of free polyamines by high-performance liquid chromatography showed that the cultures on EIM had less putrescine than those on CGM. However, increase in spermidine and spermine was detected in cultures on EIM during the first 20d of culture, coincident with abundant somatic embryogenesis. The globular embryos contained more polyamines than embryos at other stages. Semi-quantitative reverse transcriptase-polymerase chain reaction assay showed that expression levels of all of the five key genes involved in polyamine biosynthesis, with the exception of S-adenosylmethionine decarboxylase, were induced in cultures on EIM, and that their transcriptional levels were increased with maturation of the embryos. Addition of alpha-difluoromethylornithine, a polyamine biosynthesis inhibitor, to EIM resulted in remarkable inhibition of somatic embryogenesis, concurrent with notable reduction of endogenous putrescine and spermidine, particularly at higher concentrations. Exogenous application of 1mM putrescine to EIM together with 5mM alpha-difluoromethylornithine led to dramatic enhancement of endogenous polyamines, which successfully restored somatic embryogenesis. All of these, collectively, demonstrated that free polyamines, at least spermidine and spermine herein, were involved in glycerol-mediated promotion of somatic embryogenesis, which will open a new avenue for establishing a sophisticated system for somatic embryogenesis based on the modulation of endogenous polyamines.
DOI: 10.1016/j.jplph.2008.02.005
PubMed: 18448195
Affiliations:
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Le document en format XML
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<author><name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name>
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<front><div type="abstract" xml:lang="en">Culture of Citrus sinensis embryogenic callus on the embryo-inducing medium (EIM) containing glycerol gave rise to a large number of embryos, whereas very few embryos were observed on the callus growth medium (CGM). In the current paper, attempts were made to investigate whether polyamine biosynthesis was involved in glycerol-mediated somatic embryogenesis. Quantification of free polyamines by high-performance liquid chromatography showed that the cultures on EIM had less putrescine than those on CGM. However, increase in spermidine and spermine was detected in cultures on EIM during the first 20d of culture, coincident with abundant somatic embryogenesis. The globular embryos contained more polyamines than embryos at other stages. Semi-quantitative reverse transcriptase-polymerase chain reaction assay showed that expression levels of all of the five key genes involved in polyamine biosynthesis, with the exception of S-adenosylmethionine decarboxylase, were induced in cultures on EIM, and that their transcriptional levels were increased with maturation of the embryos. Addition of alpha-difluoromethylornithine, a polyamine biosynthesis inhibitor, to EIM resulted in remarkable inhibition of somatic embryogenesis, concurrent with notable reduction of endogenous putrescine and spermidine, particularly at higher concentrations. Exogenous application of 1mM putrescine to EIM together with 5mM alpha-difluoromethylornithine led to dramatic enhancement of endogenous polyamines, which successfully restored somatic embryogenesis. All of these, collectively, demonstrated that free polyamines, at least spermidine and spermine herein, were involved in glycerol-mediated promotion of somatic embryogenesis, which will open a new avenue for establishing a sophisticated system for somatic embryogenesis based on the modulation of endogenous polyamines.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><noCountry><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name>
<name sortKey="Liu, Ji Hong" sort="Liu, Ji Hong" uniqKey="Liu J" first="Ji-Hong" last="Liu">Ji-Hong Liu</name>
<name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Wu, Xiao Ba" sort="Wu, Xiao Ba" uniqKey="Wu X" first="Xiao-Ba" last="Wu">Xiao-Ba Wu</name>
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