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Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine

Identifieur interne : 002C81 ( Main/Exploration ); précédent : 002C80; suivant : 002C82

Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine

Auteurs : Jin-Hu Wu [Nouvelle-Zélande] ; Pauline Mooney [Nouvelle-Zélande]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 2002.

RBID : Pascal:02-0459190

Descripteurs français

Pascal (Inist)
- Tétraploïdie, Régénération, Haploïde doublé, Modalité traitement, Callogenèse, Effet concentration, Multiplication végétative in vitro, Nombre chromosomique, Cytométrie flux, Citrus reticulata, Citrus paradisi, Colchicine, Embryon somatique, Cytogénétique, Citrus reticulata Citrus sinensis, Oryzalin, Autoploïdie.

English descriptors

KwdEn :
- Application method, Autoploidy, Callogenesis, Chromosomic number, Citrus paradisi, Citrus reticulata, Colchicine, Concentration effect, Cytogenetics, Doubled haploid, Flow cytometry, Micropropagation, Regeneration, Somatic embryo, Tetraploidy.

Abstract

In vitro chromosome doubling of embryogenic callus lines of the Citrus cultivars 'Umatilla' and 'Dweet' tangors (Citrus reticulata Blanco×C. sinensis [L.] Osb.), 'Caffin' clementine (C. clementina Hort. ex Tan.) and 'Wheeny' grapefruit (C. paradisi Macf) was carried out in the presence of either 0.05 or 0.1% colchicine, or 0.01, 0.05 or 0.1% oryzalin. Embryogenic callus development was partly suppressed in the presence of colchicine, and completely suppressed by oryzalin at all concentrations tested. No plants were regenerated from any of the oryzalin treatments. Ploidy level of plants regenerated from the colchicine treatments was determined using flow cytometry and chromosome squashes. Three desirable non-chimeric, autotetraploid plants of the mono-embryonic cultivar 'Umatilla' were produced using 0.05% colchicine and one from 0.1% colchicine. One mixoploid 'Dweet' plant was produced using 0.1% colchicine.

Affiliations:

Nouvelle-Zélande

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to stream PascalFrancis, to step Checkpoint: 000821
to stream Main, to step Merge: 002E60
to stream Main, to step Curation: 002C81

Le document en format XML

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<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Horticulture and Food Research Institute of New Zealand, Kerikeri Research Centre, P.O. Box 23</s1>
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<author><name sortKey="Mooney, Pauline" sort="Mooney, Pauline" uniqKey="Mooney P" first="Pauline" last="Mooney">Pauline Mooney</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Horticulture and Food Research Institute of New Zealand, Kerikeri Research Centre, P.O. Box 23</s1>
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<term>Callogenesis</term>
<term>Chromosomic number</term>
<term>Citrus paradisi</term>
<term>Citrus reticulata</term>
<term>Colchicine</term>
<term>Concentration effect</term>
<term>Cytogenetics</term>
<term>Doubled haploid</term>
<term>Flow cytometry</term>
<term>Micropropagation</term>
<term>Regeneration</term>
<term>Somatic embryo</term>
<term>Tetraploidy</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Tétraploïdie</term>
<term>Régénération</term>
<term>Haploïde doublé</term>
<term>Modalité traitement</term>
<term>Callogenèse</term>
<term>Effet concentration</term>
<term>Multiplication végétative in vitro</term>
<term>Nombre chromosomique</term>
<term>Cytométrie flux</term>
<term>Citrus reticulata</term>
<term>Citrus paradisi</term>
<term>Colchicine</term>
<term>Embryon somatique</term>
<term>Cytogénétique</term>
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<term>Oryzalin</term>
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<front><div type="abstract" xml:lang="en">In vitro chromosome doubling of embryogenic callus lines of the Citrus cultivars 'Umatilla' and 'Dweet' tangors (Citrus reticulata Blanco×C. sinensis [L.] Osb.), 'Caffin' clementine (C. clementina Hort. ex Tan.) and 'Wheeny' grapefruit (C. paradisi Macf) was carried out in the presence of either 0.05 or 0.1% colchicine, or 0.01, 0.05 or 0.1% oryzalin. Embryogenic callus development was partly suppressed in the presence of colchicine, and completely suppressed by oryzalin at all concentrations tested. No plants were regenerated from any of the oryzalin treatments. Ploidy level of plants regenerated from the colchicine treatments was determined using flow cytometry and chromosome squashes. Three desirable non-chimeric, autotetraploid plants of the mono-embryonic cultivar 'Umatilla' were produced using 0.05% colchicine and one from 0.1% colchicine. One mixoploid 'Dweet' plant was produced using 0.1% colchicine.</div>
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Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine

Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri