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Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model

Identifieur interne : 000574 ( Main/Exploration ); précédent : 000573; suivant : 000575

Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model

Auteurs : Neeraj K. Patel ; Mohd. Shahid Khan ; Kamlesh K. Bhutani

Source :

EXCLI Journal [ 1611-2156 ] ; 2015.

RBID : PMC:4616245

Abstract

Silica gel column chromatography fractionation of the dichloromethane extract (LCD) of Leucas cephalotes (Roth.) Spreng. led to the isolation of five compounds namely β-sitosterol (1) + stigmasterol (2), lupeol (3), oleanolic acid (4) and laballenic acid (5). Also, gas chromatography-mass spectrometry (GC-MS) analysis of sub-fraction (LCD-F1) of this extract showed the presence of eleven (6-16) compounds. In addition to this, 3-5 and LCD-F1 were evaluated for lipopolysachharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in RAW 264.7 and J774A.1 cells. Results directed that 4 and 5 were found to inhibit these mediators at half maximal inhibitory concentration of 17.12 to 57.20 μM while IC₅₀ for LCD-F1 was found to be 15.56 to 31.71 μg/mL. Furthermore, LCD at a dose of 50, 100 and 400 mg/Kg was found to reduce significantly LPS induced tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in female Sprague Dawley (SD) rats. All the results findings evoked that the anti-inflammatory effects of Leucas cephalotes is partially mediated through the suppression of pro-inflammatory mediators and hence can be utilized for the development of anti-inflammatory candidates.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616245

DOI: 10.17179/excli2014-667
PubMed: 26535039
PubMed Central: 4616245

Affiliations:

Le document en format XML

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<affiliation><nlm:aff id="A1">Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S Nagar, Mohali-160062, Punjab (INDIA)</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p>Silica gel column chromatography fractionation of the dichloromethane extract (LCD) of <italic>Leucas cephalotes </italic>
(Roth.) Spreng. led to the isolation of five compounds namely β-sitosterol (<bold>1</bold>
) + stigmasterol (<bold>2</bold>
), lupeol (<bold>3</bold>
), oleanolic acid (<bold>4</bold>
) and laballenic acid (<bold>5</bold>
). Also, gas chromatography-mass spectrometry (GC-MS) analysis of sub-fraction (LCD-F1) of this extract showed the presence of eleven (<bold>6</bold>
-<bold>16</bold>
) compounds. In addition to this, <bold>3</bold>
-<bold>5</bold>
 and LCD-F1 were evaluated for lipopolysachharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in RAW 264.7 and J774A.1 cells. Results directed that <bold>4</bold>
 and <bold>5</bold>
 were found to inhibit these mediators at half maximal inhibitory concentration of 17.12 to 57.20 μM while IC<sub>50</sub>
 for LCD-F1 was found to be 15.56 to 31.71 μg/mL. Furthermore, LCD at a dose of 50, 100 and 400 mg/Kg was found to reduce significantly LPS induced tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in female Sprague Dawley (SD) rats. All the results findings evoked that the anti-inflammatory effects of <italic>Leucas cephalotes</italic>
 is partially mediated through the suppression of pro-inflammatory mediators and hence can be utilized for the development of anti-inflammatory candidates.</p>
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Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model

Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model

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