Ploidy of somatic embryos and the ability to regenerate plantlets in melon ( Cucumis melo L.)
Identifieur interne : 003694 ( Main/Exploration ); précédent : 003693; suivant : 003695Ploidy of somatic embryos and the ability to regenerate plantlets in melon ( Cucumis melo L.)
Auteurs : Hiroshi Ezura [Japon] ; Katsuji Oosawa [Japon]Source :
- Plant Cell Reports [ 0721-7714 ] ; 1994-12-01.
Abstract
Summary: The number of chromosomes in cells of callus, somatic embryos and regenerated plantlets during somatic embryogenesis were examined in two cultivars of melon (Cucumis melo L.). Somatic embryos were diploid (50.0%/32.1%), tetraploid (38.5%/57.5%) and octoploid (11.5%/10.4%) whereas in callus cells diploidy (41.9%/43.3%), tetraploidy (27.9%/25.8%), octoploidy (11.6%/15.5%) and a low frequency of other types of ploidy and aneuploidy were observed. Mixoploid somatic embryos were not observed. These results suggest that the somatic embryos were selectively differentiated from diploid, tetraploid and octoploid cells, and that endopolyploidization of cultured cells occurred before the start of cell division leading to somatic embryogenesis. The ratio of diploid to tetraploid (1.30/0.55) in somatic embryos was less than that in callus cells (1.50/1.68) while ratios of diploid to octoploid (4.35/3.09) and tetraploid to octoploid (3.35/5.52) in somatic embryos were greater than those in callus cells (3.61/2.80 and 2.40/1.67). Therefore, it appears that the ability of callus cell to differentiate into somatic embryos increases in the following order: octoploid < diploid < tetraploid. Regenerated plantlets were diploid (65.5%/55.1%) and tetraploid (34.5%/44.9%). No octoploid plantlets were observed. The ratio of diploid to tetraploid in regenerated plantlets (1.72/1.23) was greater than that in somatic embryos. Therefore, it appears that the ability of somatic embryos to develop into plantlets increases in the following order: octoploid < tetraploid < diploid.
Url:
DOI: 10.1007/BF00233771
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ploidy of somatic embryos and the ability to regenerate plantlets in melon ( Cucumis melo L.)</title><author><name sortKey="Ezura, Hiroshi" sort="Ezura, Hiroshi" uniqKey="Ezura H" first="Hiroshi" last="Ezura">Hiroshi Ezura</name></author><author><name sortKey="Oosawa, Katsuji" sort="Oosawa, Katsuji" uniqKey="Oosawa K" first="Katsuji" last="Oosawa">Katsuji Oosawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:023C118157A64CC3ACBB4F000B99F7DD1613EF98</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1007/BF00233771</idno><idno type="url">https://api.istex.fr/document/023C118157A64CC3ACBB4F000B99F7DD1613EF98/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E81</idno><idno type="wicri:Area/Istex/Curation">000E81</idno><idno type="wicri:Area/Istex/Checkpoint">000F51</idno><idno type="wicri:doubleKey">0721-7714:1994:Ezura H:ploidy:of:somatic</idno><idno type="wicri:Area/Main/Merge">003974</idno><idno type="wicri:Area/Main/Curation">003694</idno><idno type="wicri:Area/Main/Exploration">003694</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Ploidy of somatic embryos and the ability to regenerate plantlets in melon ( Cucumis melo L.)</title><author><name sortKey="Ezura, Hiroshi" sort="Ezura, Hiroshi" uniqKey="Ezura H" first="Hiroshi" last="Ezura">Hiroshi Ezura</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Plant Biotechnology Institute, IBARAKI Agricultural Center, Ago, Iwama, 319-02, Nishi-Ibaraki</wicri:regionArea><wicri:noRegion>Nishi-Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Oosawa, Katsuji" sort="Oosawa, Katsuji" uniqKey="Oosawa K" first="Katsuji" last="Oosawa">Katsuji Oosawa</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Plant Biotechnology Institute, IBARAKI Agricultural Center, Ago, Iwama, 319-02, Nishi-Ibaraki</wicri:regionArea><wicri:noRegion>Nishi-Ibaraki</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Cell Reports</title><title level="j" type="abbrev">Plant Cell Reports</title><idno type="ISSN">0721-7714</idno><idno type="eISSN">1432-203X</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1994-12-01">1994-12-01</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">2-3</biblScope><biblScope unit="page" from="107">107</biblScope><biblScope unit="page" to="111">111</biblScope></imprint><idno type="ISSN">0721-7714</idno></series><idno type="istex">023C118157A64CC3ACBB4F000B99F7DD1613EF98</idno><idno type="DOI">10.1007/BF00233771</idno><idno type="ArticleID">BF00233771</idno><idno type="ArticleID">Art8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The number of chromosomes in cells of callus, somatic embryos and regenerated plantlets during somatic embryogenesis were examined in two cultivars of melon (Cucumis melo L.). Somatic embryos were diploid (50.0%/32.1%), tetraploid (38.5%/57.5%) and octoploid (11.5%/10.4%) whereas in callus cells diploidy (41.9%/43.3%), tetraploidy (27.9%/25.8%), octoploidy (11.6%/15.5%) and a low frequency of other types of ploidy and aneuploidy were observed. Mixoploid somatic embryos were not observed. These results suggest that the somatic embryos were selectively differentiated from diploid, tetraploid and octoploid cells, and that endopolyploidization of cultured cells occurred before the start of cell division leading to somatic embryogenesis. The ratio of diploid to tetraploid (1.30/0.55) in somatic embryos was less than that in callus cells (1.50/1.68) while ratios of diploid to octoploid (4.35/3.09) and tetraploid to octoploid (3.35/5.52) in somatic embryos were greater than those in callus cells (3.61/2.80 and 2.40/1.67). Therefore, it appears that the ability of callus cell to differentiate into somatic embryos increases in the following order: octoploid < diploid < tetraploid. Regenerated plantlets were diploid (65.5%/55.1%) and tetraploid (34.5%/44.9%). No octoploid plantlets were observed. The ratio of diploid to tetraploid in regenerated plantlets (1.72/1.23) was greater than that in somatic embryos. Therefore, it appears that the ability of somatic embryos to develop into plantlets increases in the following order: octoploid < tetraploid < diploid.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Ezura, Hiroshi" sort="Ezura, Hiroshi" uniqKey="Ezura H" first="Hiroshi" last="Ezura">Hiroshi Ezura</name></noRegion><name sortKey="Oosawa, Katsuji" sort="Oosawa, Katsuji" uniqKey="Oosawa K" first="Katsuji" last="Oosawa">Katsuji Oosawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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