Citrus rootstock responses to herbivory by larvae of the sugarcane rootstock borer weevil (Diaprepes abbreviatus)
Identifieur interne : 003647 ( Main/Exploration ); précédent : 003646; suivant : 003648Citrus rootstock responses to herbivory by larvae of the sugarcane rootstock borer weevil (Diaprepes abbreviatus)
Auteurs : R. T. Mayer [États-Unis] ; J. P. Shapiro ; E. Berdis ; C. J. Hearn ; T. G. Mccollum ; R. E. Mcdonald ; H. DoostdarSource :
- Physiologia Plantarum : (København. 1948) [ 0031-9317 ] ; 1995.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The responses of roots to feeding by larvae of a citrus root weevil (Diaprepes abbreviatus) were investigated in Citrus grandis (L.) Osb.×Poncirus trifoliata (2N) (L.) Raf.; C. grandis×P. trifoliata (4N); P. trifoliata×C. grandis (Flying Dragon×Nakon); C. paradisi Macf.×P. trifoliana (Swingle citrumelo); C. aurantifolia (Christm.) Swingle (Citrus macrophylla); C. reticulata Blanco (Cleopatra mandarin); C. sinensis (L.) Osb.×P. trifoliata (Carrizo citrange); C. auranticum (L.) (sour orange). Chitinase, chitosanase, β-1,3-glucanase, peroxidase and lysozyme activities were measured and significant differences were observed for some of the cultivars between infested and uninfested rootstocks. Generally, increased activities were observed for chitinases and decreased activities were observed for the other enzymes measured. Numerous significant differences in hydrolase and peroxidase activities were observed between cultivars. Immunological detection revealed that new protein bands occurred in root protein extracts for six of the eight cultivars infested with larvae when an antibody to a class I potato leaf chitinase was used. Antibodies generated against two citrus chitinases of Mr 24 000 (basic chitinase cv. Valencia (C. sinensis) callus, BCVC) and Mr 28 000 (basic chitinase/lysozyme cv. Valencia callus, BCLVC) indicated that chitinases in Carrizo were induced in infested roots when the BCVC antibody was employed. These findings justify calling these proteins pathogenesis-related proteins. The chitinase that BCLVC was prepared from exhibited high lysozyme activities, and the results of western blots showed the presence of proteins at Mr 24 000 and 27 000 which are presumed to be lysozymes. Similar tests using antibodies against β-1,3-glucanases and peroxidases indicated a diminution of protein bands that cross-reacted with infested root protein extracts compared with what occurred in controls. All of the root extracts were tested against chitosans with various percentages of acetylation; activities were linearly dependent on the amount of chitosan acetylation; i.e. the larger the amount of acetylation, the greater the activity. Significant differences in hydrolase activities were observed between infested and uninfested roots for the rootstocks using the variously acetylated substrates. All of the root protein extracts were capable of degrading peritrophic membranes removed from larvae of D. abbreviatus. This suggests that citrus chitinases may play a role in disrupting the peritrophic membrane such that ingested substances that pose a hazard to the insect may penetrate the membrane more easily
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Hearn</name></author><author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T. G." last="Mccollum">T. G. Mccollum</name></author><author><name sortKey="Mcdonald, R E" sort="Mcdonald, R E" uniqKey="Mcdonald R" first="R. E." last="Mcdonald">R. E. Mcdonald</name></author><author><name sortKey="Doostdar, H" sort="Doostdar, H" uniqKey="Doostdar H" first="H." last="Doostdar">H. 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Mayer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>ARS, US horticultural res. lab.</s1><s2>Orlando FL 32803</s2><s3>USA</s3></inist:fA14><country>États-Unis</country><wicri:noRegion>Orlando FL 32803</wicri:noRegion></affiliation></author><author><name sortKey="Shapiro, J P" sort="Shapiro, J P" uniqKey="Shapiro J" first="J. P." last="Shapiro">J. P. Shapiro</name></author><author><name sortKey="Berdis, E" sort="Berdis, E" uniqKey="Berdis E" first="E." last="Berdis">E. Berdis</name></author><author><name sortKey="Hearn, C J" sort="Hearn, C J" uniqKey="Hearn C" first="C. J." last="Hearn">C. J. Hearn</name></author><author><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T. G." last="Mccollum">T. G. Mccollum</name></author><author><name sortKey="Mcdonald, R E" sort="Mcdonald, R E" uniqKey="Mcdonald R" first="R. E." last="Mcdonald">R. E. Mcdonald</name></author><author><name sortKey="Doostdar, H" sort="Doostdar, H" uniqKey="Doostdar H" first="H." last="Doostdar">H. Doostdar</name></author></analytic><series><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno><imprint><date when="1995">1995</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chitinase</term><term>Chitosanase</term><term>Cultivar</term><term>Diaprepes abbreviatus</term><term>Enzymatic activity</term><term>Glucanase</term><term>Host agent relation</term><term>Pathogenesis related protein</term><term>Peroxidase</term><term>Root</term><term>Rootstock</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cultivar</term><term>Racine</term><term>Chitinase</term><term>Chitosanase</term><term>Peroxidase</term><term>Glucanase</term><term>Activité enzymatique</term><term>Protéine PR</term><term>Relation hôte agent</term><term>Porte greffe</term><term>Diaprepes abbreviatus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The responses of roots to feeding by larvae of a citrus root weevil (Diaprepes abbreviatus) were investigated in Citrus grandis (L.) Osb.×Poncirus trifoliata (2N) (L.) Raf.; C. grandis×P. trifoliata (4N); P. trifoliata×C. grandis (Flying Dragon×Nakon); C. paradisi Macf.×P. trifoliana (Swingle citrumelo); C. aurantifolia (Christm.) Swingle (Citrus macrophylla); C. reticulata Blanco (Cleopatra mandarin); C. sinensis (L.) Osb.×P. trifoliata (Carrizo citrange); C. auranticum (L.) (sour orange). Chitinase, chitosanase, β-1,3-glucanase, peroxidase and lysozyme activities were measured and significant differences were observed for some of the cultivars between infested and uninfested rootstocks. Generally, increased activities were observed for chitinases and decreased activities were observed for the other enzymes measured. Numerous significant differences in hydrolase and peroxidase activities were observed between cultivars. Immunological detection revealed that new protein bands occurred in root protein extracts for six of the eight cultivars infested with larvae when an antibody to a class I potato leaf chitinase was used. Antibodies generated against two citrus chitinases of M<sub>r</sub> 24 000 (basic chitinase cv. Valencia (C. sinensis) callus, BCVC) and M<sub>r</sub> 28 000 (basic chitinase/lysozyme cv. Valencia callus, BCLVC) indicated that chitinases in Carrizo were induced in infested roots when the BCVC antibody was employed. These findings justify calling these proteins pathogenesis-related proteins. The chitinase that BCLVC was prepared from exhibited high lysozyme activities, and the results of western blots showed the presence of proteins at M<sub>r</sub> 24 000 and 27 000 which are presumed to be lysozymes. Similar tests using antibodies against β-1,3-glucanases and peroxidases indicated a diminution of protein bands that cross-reacted with infested root protein extracts compared with what occurred in controls. All of the root extracts were tested against chitosans with various percentages of acetylation; activities were linearly dependent on the amount of chitosan acetylation; i.e. the larger the amount of acetylation, the greater the activity. Significant differences in hydrolase activities were observed between infested and uninfested roots for the rootstocks using the variously acetylated substrates. All of the root protein extracts were capable of degrading peritrophic membranes removed from larvae of D. abbreviatus. This suggests that citrus chitinases may play a role in disrupting the peritrophic membrane such that ingested substances that pose a hazard to the insect may penetrate the membrane more easily</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Berdis, E" sort="Berdis, E" uniqKey="Berdis E" first="E." last="Berdis">E. Berdis</name><name sortKey="Doostdar, H" sort="Doostdar, H" uniqKey="Doostdar H" first="H." last="Doostdar">H. Doostdar</name><name sortKey="Hearn, C J" sort="Hearn, C J" uniqKey="Hearn C" first="C. J." last="Hearn">C. J. Hearn</name><name sortKey="Mccollum, T G" sort="Mccollum, T G" uniqKey="Mccollum T" first="T. G." last="Mccollum">T. G. Mccollum</name><name sortKey="Mcdonald, R E" sort="Mcdonald, R E" uniqKey="Mcdonald R" first="R. E." last="Mcdonald">R. E. Mcdonald</name><name sortKey="Shapiro, J P" sort="Shapiro, J P" uniqKey="Shapiro J" first="J. P." last="Shapiro">J. P. Shapiro</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Mayer, R T" sort="Mayer, R T" uniqKey="Mayer R" first="R. T." last="Mayer">R. T. Mayer</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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