Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast-protoplast fusion
Identifieur interne : 002645 ( Main/Exploration ); précédent : 002644; suivant : 002646Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast-protoplast fusion
Auteurs : X. Y. Xu [République populaire de Chine] ; J. H. Liu [République populaire de Chine] ; X. X. Deng [République populaire de Chine]Source :
- Plant cell reports : (Print) [ 0721-7714 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Production.
English descriptors
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Abstract
Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present paper cytoplasts were isolated from cell suspension-derived protoplasts of Satsuma mandarin via ultra-centrifugation in a discontinuous gradient. No nucleus could be detected in the cytoplasts by DAPI (4', 6-diamidino-2-phenylindole) staining compared with normal protoplasts. The cytoplasts, with high viability and small size, did not divide during solid embedding culture. Cytoplasts of Satsuma mandarin were electrically fused with embryogenic protoplasts of Murcott tangor (C. reticulata x C. sinensis), which led to regeneration of several cell lines. Flow cytometry (FCM) indicated that the cell lines were diploids. Simple sequence repeats (SSR) and cleaved amplified polymorphism sequence (CAPS) showed that the cell lines got their nuclear DNA from the protoplast parent, whereas the cytoplast parent donated the mtDNA, confirming transfer of mtDNA from Satsuma mandarin into Murcott tangor via cytoplast-protoplast fusion though no polymorphism was detected in chloroplast DNA between the fusion partners. This is the first report on isolation and characterization of cytoplasts, together with cytoplast-protoplast fusion in Citrus, which has a potential for citrus cultivar improvement involving cytoplasm transfer via cytoplast-protoplast fusion.
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Liu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Deng, X X" sort="Deng, X X" uniqKey="Deng X" first="X. X." last="Deng">X. X. Deng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0541578</idno><date when="2006">2006</date><idno type="stanalyst">PASCAL 06-0541578 INIST</idno><idno type="RBID">Pascal:06-0541578</idno><idno type="wicri:Area/PascalFrancis/Corpus">000522</idno><idno type="wicri:Area/PascalFrancis/Curation">000482</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000539</idno><idno type="wicri:doubleKey">0721-7714:2006:Xu X:isolation:of:cytoplasts</idno><idno type="wicri:Area/Main/Merge">002754</idno><idno type="wicri:Area/Main/Curation">002645</idno><idno type="wicri:Area/Main/Exploration">002645</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast-protoplast fusion</title><author><name sortKey="Xu, X Y" sort="Xu, X Y" uniqKey="Xu X" first="X. Y." last="Xu">X. Y. Xu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Liu, J H" sort="Liu, J H" uniqKey="Liu J" first="J. H." last="Liu">J. H. Liu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Deng, X X" sort="Deng, X X" uniqKey="Deng X" first="X. X." last="Deng">X. X. Deng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant cell reports : (Print)</title><title level="j" type="abbreviated">Plant cell rep. : (Print)</title><idno type="ISSN">0721-7714</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant cell reports : (Print)</title><title level="j" type="abbreviated">Plant cell rep. : (Print)</title><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell fusion</term><term>Citrus unshiu</term><term>Cytoplast</term><term>Hybrid</term><term>Production</term><term>Protoplast</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cytoplaste</term><term>Production</term><term>Hybride</term><term>Fusion cellulaire</term><term>Protoplaste</term><term>Citrus unshiu</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present paper cytoplasts were isolated from cell suspension-derived protoplasts of Satsuma mandarin via ultra-centrifugation in a discontinuous gradient. No nucleus could be detected in the cytoplasts by DAPI (4', 6-diamidino-2-phenylindole) staining compared with normal protoplasts. The cytoplasts, with high viability and small size, did not divide during solid embedding culture. Cytoplasts of Satsuma mandarin were electrically fused with embryogenic protoplasts of Murcott tangor (C. reticulata x C. sinensis), which led to regeneration of several cell lines. Flow cytometry (FCM) indicated that the cell lines were diploids. Simple sequence repeats (SSR) and cleaved amplified polymorphism sequence (CAPS) showed that the cell lines got their nuclear DNA from the protoplast parent, whereas the cytoplast parent donated the mtDNA, confirming transfer of mtDNA from Satsuma mandarin into Murcott tangor via cytoplast-protoplast fusion though no polymorphism was detected in chloroplast DNA between the fusion partners. This is the first report on isolation and characterization of cytoplasts, together with cytoplast-protoplast fusion in Citrus, which has a potential for citrus cultivar improvement involving cytoplasm transfer via cytoplast-protoplast fusion.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, X Y" sort="Xu, X Y" uniqKey="Xu X" first="X. Y." last="Xu">X. Y. Xu</name></noRegion><name sortKey="Deng, X X" sort="Deng, X X" uniqKey="Deng X" first="X. X." last="Deng">X. X. Deng</name><name sortKey="Liu, J H" sort="Liu, J H" uniqKey="Liu J" first="J. H." last="Liu">J. H. Liu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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