Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene
Identifieur interne : 002393 ( Main/Exploration ); précédent : 002392; suivant : 002394Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene
Auteurs : A. Gentile [Italie] ; Z. Deng [République populaire de Chine] ; S. La Malfa [Italie] ; G. Distefano [Italie] ; F. Domina [Italie] ; A. Vitale [Italie] ; G. Polizzi [Italie] ; M. Lorito [Italie] ; E. Tribulato [Italie]Source :
- Plant Breeding [ 0179-9541 ] ; 2007-04.
English descriptors
- KwdEn :
Abstract
Antifungal genes from micro‐organisms were inserted into the genome of a number of plant species, representing a promising strategy for conferring genetic disease resistance against a broad range of plant pathogenic fungi. In the present study, the chit42 gene from Trichoderma harzianum (codifying the antifungal protein endochitinase) was introduced into the ‘Femminello siracusano’ lemon by Agrobacterium tumefaciens, in order to regenerate transgenic plants resistant to fungal disease. Three polymerase chain reaction (PCR)‐positive clones were obtained. Southern blot confirmed the integration of the transgene in the lemon genome and revealed that one or two copies had been inserted. Reverse transcriptase‐PCR, Northern blot and Western analysis were performed and the results confirmed the expression of the inserted gene. The transgenic clones were tested in vitro and in vivo for disease resistance. Conidia germination and fungal growth of Phoma tracheiphila were strongly inhibited in vitro by the transgenic foliar proteins, while no effects were observed with the controls. Disease resistance assays were performed in vivo with Botrytis cinerea, the causal agent of grey mould in fruit. Transgenic lemon plants, inoculated with lemon petals infected by a single‐conidial isolate of B. cinerea, showed significantly less lesion development than the controls. On the whole, the results indicate that the transformation with the antifungal endochitinase gene may represent a strategy for disease control in citrus crops.
Url:
DOI: 10.1111/j.1439-0523.2007.01297.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000D91
- to stream Istex, to step Curation: 000D91
- to stream Istex, to step Checkpoint: 000671
- to stream Main, to step Merge: 002477
- to stream Main, to step Curation: 002393
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene</title>
<author><name sortKey="Gentile, A" sort="Gentile, A" uniqKey="Gentile A" first="A." last="Gentile">A. Gentile</name>
</author>
<author><name sortKey="Deng, Z" sort="Deng, Z" uniqKey="Deng Z" first="Z." last="Deng">Z. Deng</name>
</author>
<author><name sortKey="La Malfa, S" sort="La Malfa, S" uniqKey="La Malfa S" first="S." last="La Malfa">S. La Malfa</name>
</author>
<author><name sortKey="Distefano, G" sort="Distefano, G" uniqKey="Distefano G" first="G." last="Distefano">G. Distefano</name>
</author>
<author><name sortKey="Domina, F" sort="Domina, F" uniqKey="Domina F" first="F." last="Domina">F. Domina</name>
</author>
<author><name sortKey="Vitale, A" sort="Vitale, A" uniqKey="Vitale A" first="A." last="Vitale">A. Vitale</name>
</author>
<author><name sortKey="Polizzi, G" sort="Polizzi, G" uniqKey="Polizzi G" first="G." last="Polizzi">G. Polizzi</name>
</author>
<author><name sortKey="Lorito, M" sort="Lorito, M" uniqKey="Lorito M" first="M." last="Lorito">M. Lorito</name>
</author>
<author><name sortKey="Tribulato, E" sort="Tribulato, E" uniqKey="Tribulato E" first="E." last="Tribulato">E. Tribulato</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A48B75478EDD32FB62EEF77A272404247B827610</idno>
<date when="2007" year="2007">2007</date>
<idno type="doi">10.1111/j.1439-0523.2007.01297.x</idno>
<idno type="url">https://api.istex.fr/document/A48B75478EDD32FB62EEF77A272404247B827610/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D91</idno>
<idno type="wicri:Area/Istex/Curation">000D91</idno>
<idno type="wicri:Area/Istex/Checkpoint">000671</idno>
<idno type="wicri:doubleKey">0179-9541:2007:Gentile A:enhanced:resistance:to</idno>
<idno type="wicri:Area/Main/Merge">002477</idno>
<idno type="wicri:Area/Main/Curation">002393</idno>
<idno type="wicri:Area/Main/Exploration">002393</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene</title>
<author><name sortKey="Gentile, A" sort="Gentile, A" uniqKey="Gentile A" first="A." last="Gentile">A. Gentile</name>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
<wicri:regionArea>Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Catania I‐95123, Italy</wicri:regionArea>
<wicri:noRegion>Italy</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Deng, Z" sort="Deng, Z" uniqKey="Deng Z" first="Z." last="Deng">Z. Deng</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Horticulture and Landscape College, Hunan Agricultural University, Changsha, Hunan 410128</wicri:regionArea>
<wicri:noRegion>Hunan 410128</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="La Malfa, S" sort="La Malfa, S" uniqKey="La Malfa S" first="S." last="La Malfa">S. La Malfa</name>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
<wicri:regionArea>Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Catania I‐95123, Italy</wicri:regionArea>
<wicri:noRegion>Italy</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Distefano, G" sort="Distefano, G" uniqKey="Distefano G" first="G." last="Distefano">G. Distefano</name>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
<wicri:regionArea>Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Catania I‐95123, Italy</wicri:regionArea>
<wicri:noRegion>Italy</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Domina, F" sort="Domina, F" uniqKey="Domina F" first="F." last="Domina">F. Domina</name>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
<wicri:regionArea>Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Catania I‐95123, Italy</wicri:regionArea>
<wicri:noRegion>Italy</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vitale, A" sort="Vitale, A" uniqKey="Vitale A" first="A." last="Vitale">A. Vitale</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Scienze e Tecnologie Fitosanitarie, University of Catania, I‐95123 Catania</wicri:regionArea>
<wicri:noRegion>I‐95123 Catania</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Polizzi, G" sort="Polizzi, G" uniqKey="Polizzi G" first="G." last="Polizzi">G. Polizzi</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Scienze e Tecnologie Fitosanitarie, University of Catania, I‐95123 Catania</wicri:regionArea>
<wicri:noRegion>I‐95123 Catania</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lorito, M" sort="Lorito, M" uniqKey="Lorito M" first="M." last="Lorito">M. Lorito</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Arboricoltura e Patologia Vegetale, University of Naples, I‐80055 Portici (NA)</wicri:regionArea>
<wicri:noRegion>I‐80055 Portici (NA)</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tribulato, E" sort="Tribulato, E" uniqKey="Tribulato E" first="E." last="Tribulato">E. Tribulato</name>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
<wicri:regionArea>Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Catania I‐95123, Italy</wicri:regionArea>
<wicri:noRegion>Italy</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Breeding</title>
<idno type="ISSN">0179-9541</idno>
<idno type="eISSN">1439-0523</idno>
<imprint><publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="2007-04">2007-04</date>
<biblScope unit="volume">126</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="146">146</biblScope>
<biblScope unit="page" to="151">151</biblScope>
</imprint>
<idno type="ISSN">0179-9541</idno>
</series>
<idno type="istex">A48B75478EDD32FB62EEF77A272404247B827610</idno>
<idno type="DOI">10.1111/j.1439-0523.2007.01297.x</idno>
<idno type="ArticleID">PBR1297</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0179-9541</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Botrytis cinerea</term>
<term>Citrus limon</term>
<term>Phoma tracheiphila</term>
<term>chit42 antifungal gene</term>
<term>disease resistance assays</term>
<term>grey mould</term>
<term>malsecco</term>
<term>transgene expression</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Antifungal genes from micro‐organisms were inserted into the genome of a number of plant species, representing a promising strategy for conferring genetic disease resistance against a broad range of plant pathogenic fungi. In the present study, the chit42 gene from Trichoderma harzianum (codifying the antifungal protein endochitinase) was introduced into the ‘Femminello siracusano’ lemon by Agrobacterium tumefaciens, in order to regenerate transgenic plants resistant to fungal disease. Three polymerase chain reaction (PCR)‐positive clones were obtained. Southern blot confirmed the integration of the transgene in the lemon genome and revealed that one or two copies had been inserted. Reverse transcriptase‐PCR, Northern blot and Western analysis were performed and the results confirmed the expression of the inserted gene. The transgenic clones were tested in vitro and in vivo for disease resistance. Conidia germination and fungal growth of Phoma tracheiphila were strongly inhibited in vitro by the transgenic foliar proteins, while no effects were observed with the controls. Disease resistance assays were performed in vivo with Botrytis cinerea, the causal agent of grey mould in fruit. Transgenic lemon plants, inoculated with lemon petals infected by a single‐conidial isolate of B. cinerea, showed significantly less lesion development than the controls. On the whole, the results indicate that the transformation with the antifungal endochitinase gene may represent a strategy for disease control in citrus crops.</div>
</front>
</TEI>
<affiliations><list><country><li>Italie</li>
<li>République populaire de Chine</li>
</country>
</list>
<tree><country name="Italie"><noRegion><name sortKey="Gentile, A" sort="Gentile, A" uniqKey="Gentile A" first="A." last="Gentile">A. Gentile</name>
</noRegion>
<name sortKey="Distefano, G" sort="Distefano, G" uniqKey="Distefano G" first="G." last="Distefano">G. Distefano</name>
<name sortKey="Domina, F" sort="Domina, F" uniqKey="Domina F" first="F." last="Domina">F. Domina</name>
<name sortKey="La Malfa, S" sort="La Malfa, S" uniqKey="La Malfa S" first="S." last="La Malfa">S. La Malfa</name>
<name sortKey="Lorito, M" sort="Lorito, M" uniqKey="Lorito M" first="M." last="Lorito">M. Lorito</name>
<name sortKey="Polizzi, G" sort="Polizzi, G" uniqKey="Polizzi G" first="G." last="Polizzi">G. Polizzi</name>
<name sortKey="Tribulato, E" sort="Tribulato, E" uniqKey="Tribulato E" first="E." last="Tribulato">E. Tribulato</name>
<name sortKey="Vitale, A" sort="Vitale, A" uniqKey="Vitale A" first="A." last="Vitale">A. Vitale</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Deng, Z" sort="Deng, Z" uniqKey="Deng Z" first="Z." last="Deng">Z. Deng</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002393 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002393 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:A48B75478EDD32FB62EEF77A272404247B827610 |texte= Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene }}
This area was generated with Dilib version V0.6.25. |