Grape berry plasma membrane proteome analysis and its differential expression during ripening
Identifieur interne : 002106 ( Main/Exploration ); précédent : 002105; suivant : 002107Grape berry plasma membrane proteome analysis and its differential expression during ripening
Auteurs : Jiangwei Zhang [République populaire de Chine] ; Huiqin Ma [République populaire de Chine] ; Jidong Feng [République populaire de Chine] ; Lei Zeng [République populaire de Chine] ; Zeng Wang [République populaire de Chine] ; Shangwu Chen [République populaire de Chine]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2008-06-11.
Abstract
High purity berry plasma membranes (PMs) of Vitis vinifera L. cv. Cabernet Sauvignon were isolated by two-phase partitioning of microsome fractions at different stages of berry ripening. PM proteins resolvable by the detergent cocktail of CHAPS and ASB-14 were separated by two-dimensional electrophoresis. A total of 119 protein spots from pre-vraison berry PMs on 2-D gels detected with silver staining were subjected to MALDI-TOF mass spectrometry analysis. Sixty-two spots were identified as putative PM proteins, with 16 predicted transmembrane helices, including true PM proteins such as ATP synthase, ABC transporters, and GTP-binding proteins reported in plants. They were then grouped into eight functional categories, mainly involved in transport, metabolism, signal transduction, and protein synthesis. Another 11 spots were identified as proteins of unknown function. The vraison and post-vraison samples stained 98 and 86 spots on the gels, respectively. During the berry ripening process, total PM protein content gradually decreased. Among all identified proteins, 12 showed significant differences in terms of their relative abundance. Increasing ubiquitin proteolysis and cytoskeleton proteins were observed from pre-vraison to post-vraison. Zeatin O-glucosyltransferase peaked at vraison, while ubiquitin-conjugating enzyme E2-21 was down-regulated at this stage. This proteome research provides the first information on PM protein characterization during the grape berry ripening process.
Url:
DOI: 10.1093/jxb/ern156
Affiliations:
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uniqKey="Chen S" first="Shangwu" last="Chen">Shangwu Chen</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Food Science and Nutritional Engineering, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-06-11">2008-06-11</date><biblScope unit="volume">59</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="2979">2979</biblScope><biblScope unit="page" to="2990">2990</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno 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Cabernet Sauvignon were isolated by two-phase partitioning of microsome fractions at different stages of berry ripening. PM proteins resolvable by the detergent cocktail of CHAPS and ASB-14 were separated by two-dimensional electrophoresis. A total of 119 protein spots from pre-vraison berry PMs on 2-D gels detected with silver staining were subjected to MALDI-TOF mass spectrometry analysis. Sixty-two spots were identified as putative PM proteins, with 16 predicted transmembrane helices, including true PM proteins such as ATP synthase, ABC transporters, and GTP-binding proteins reported in plants. They were then grouped into eight functional categories, mainly involved in transport, metabolism, signal transduction, and protein synthesis. Another 11 spots were identified as proteins of unknown function. The vraison and post-vraison samples stained 98 and 86 spots on the gels, respectively. During the berry ripening process, total PM protein content gradually decreased. Among all identified proteins, 12 showed significant differences in terms of their relative abundance. Increasing ubiquitin proteolysis and cytoskeleton proteins were observed from pre-vraison to post-vraison. Zeatin O-glucosyltransferase peaked at vraison, while ubiquitin-conjugating enzyme E2-21 was down-regulated at this stage. This proteome research provides the first information on PM protein characterization during the grape berry ripening process.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Jiangwei" sort="Zhang, Jiangwei" uniqKey="Zhang J" first="Jiangwei" last="Zhang">Jiangwei Zhang</name></noRegion><name sortKey="Chen, Shangwu" sort="Chen, Shangwu" uniqKey="Chen S" first="Shangwu" last="Chen">Shangwu Chen</name><name sortKey="Chen, Shangwu" sort="Chen, Shangwu" uniqKey="Chen S" first="Shangwu" last="Chen">Shangwu Chen</name><name sortKey="Feng, Jidong" sort="Feng, Jidong" uniqKey="Feng J" first="Jidong" last="Feng">Jidong Feng</name><name sortKey="Ma, Huiqin" sort="Ma, Huiqin" uniqKey="Ma H" first="Huiqin" last="Ma">Huiqin Ma</name><name sortKey="Wang, Zeng" sort="Wang, Zeng" uniqKey="Wang Z" first="Zeng" last="Wang">Zeng Wang</name><name sortKey="Zeng, Lei" sort="Zeng, Lei" uniqKey="Zeng L" first="Lei" last="Zeng">Lei Zeng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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