Structure of the Cytosolic Part of the Subunit b-Dimer of Escherichia coli F0F1-ATP Synthase
Identifieur interne : 002009 ( Main/Exploration ); précédent : 002008; suivant : 002010Structure of the Cytosolic Part of the Subunit b-Dimer of Escherichia coli F0F1-ATP Synthase
Auteurs : Tassilo Hornung ; Oleg A. Volkov ; Tarek M. A. Zaida ; Sabine Delannoy ; John G. Wise ; Pia D. VogelSource :
- Biophysical Journal [ 0006-3495 ] ; 2008.
Abstract
The structure of the external stalk and its function in the catalytic mechanism of the F0F1-ATP synthase remains one of the important questions in bioenergetics. The external stalk has been proposed to be either a rigid stator that binds F1 or an elastic structural element that transmits energy from the small rotational steps of subunits
Url:
DOI: 10.1529/biophysj.107.121038
PubMed: 18326647
PubMed Central: 2397329
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structure of the Cytosolic Part of the Subunit <italic>b</italic>-Dimer of <italic>Escherichia coli</italic> F<sub>0</sub>F<sub>1</sub>-ATP Synthase</title><author><name sortKey="Hornung, Tassilo" sort="Hornung, Tassilo" uniqKey="Hornung T" first="Tassilo" last="Hornung">Tassilo Hornung</name></author><author><name sortKey="Volkov, Oleg A" sort="Volkov, Oleg A" uniqKey="Volkov O" first="Oleg A." last="Volkov">Oleg A. Volkov</name></author><author><name sortKey="Zaida, Tarek M A" sort="Zaida, Tarek M A" uniqKey="Zaida T" first="Tarek M. A." last="Zaida">Tarek M. A. Zaida</name></author><author><name sortKey="Delannoy, Sabine" sort="Delannoy, Sabine" uniqKey="Delannoy S" first="Sabine" last="Delannoy">Sabine Delannoy</name></author><author><name sortKey="Wise, John G" sort="Wise, John G" uniqKey="Wise J" first="John G." last="Wise">John G. Wise</name></author><author><name sortKey="Vogel, Pia D" sort="Vogel, Pia D" uniqKey="Vogel P" first="Pia D." last="Vogel">Pia D. Vogel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18326647</idno><idno type="pmc">2397329</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397329</idno><idno type="RBID">PMC:2397329</idno><idno type="doi">10.1529/biophysj.107.121038</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000767</idno><idno type="wicri:Area/Pmc/Curation">000766</idno><idno type="wicri:Area/Pmc/Checkpoint">001056</idno><idno type="wicri:Area/Ncbi/Merge">000804</idno><idno type="wicri:Area/Ncbi/Curation">000804</idno><idno type="wicri:Area/Ncbi/Checkpoint">000804</idno><idno type="wicri:doubleKey">0006-3495:2008:Hornung T:structure:of:the</idno><idno type="wicri:Area/Main/Merge">002063</idno><idno type="wicri:Area/Main/Curation">002009</idno><idno type="wicri:Area/Main/Exploration">002009</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structure of the Cytosolic Part of the Subunit <italic>b</italic>-Dimer of <italic>Escherichia coli</italic> F<sub>0</sub>F<sub>1</sub>-ATP Synthase</title><author><name sortKey="Hornung, Tassilo" sort="Hornung, Tassilo" uniqKey="Hornung T" first="Tassilo" last="Hornung">Tassilo Hornung</name></author><author><name sortKey="Volkov, Oleg A" sort="Volkov, Oleg A" uniqKey="Volkov O" first="Oleg A." last="Volkov">Oleg A. Volkov</name></author><author><name sortKey="Zaida, Tarek M A" sort="Zaida, Tarek M A" uniqKey="Zaida T" first="Tarek M. A." last="Zaida">Tarek M. A. Zaida</name></author><author><name sortKey="Delannoy, Sabine" sort="Delannoy, Sabine" uniqKey="Delannoy S" first="Sabine" last="Delannoy">Sabine Delannoy</name></author><author><name sortKey="Wise, John G" sort="Wise, John G" uniqKey="Wise J" first="John G." last="Wise">John G. Wise</name></author><author><name sortKey="Vogel, Pia D" sort="Vogel, Pia D" uniqKey="Vogel P" first="Pia D." last="Vogel">Pia D. Vogel</name></author></analytic><series><title level="j">Biophysical Journal</title><idno type="ISSN">0006-3495</idno><idno type="eISSN">1542-0086</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The structure of the external stalk and its function in the catalytic mechanism of the F<sub>0</sub>F<sub>1</sub>-ATP synthase remains one of the important questions in bioenergetics. The external stalk has been proposed to be either a rigid stator that binds F<sub>1</sub> or an elastic structural element that transmits energy from the small rotational steps of subunits <italic>c</italic> to the F<sub>1</sub> sector during catalysis. We employed proteomics, sequence-based structure prediction, molecular modeling, and electron spin resonance spectroscopy using site-directed spin labeling to understand the structure and interfacial packing of the <italic>Escherichia</italic> <italic>coli b</italic>-subunit homodimer external stalk. Comparisons of bacterial, cyanobacterial, and plant <italic>b</italic>-subunits demonstrated little sequence similarity. Supersecondary structure predictions, however, show that all compared <italic>b</italic>-sequences have extensive heptad repeats, suggesting that the proteins all are capable of packing as left-handed coiled-coils. Molecular modeling subsequently indicated that <italic>b</italic><sub>2</sub> from the <italic>E. coli</italic> ATP synthase could pack into stable left-handed coiled-coils. Thirty-eight substitutions to cysteine in soluble <italic>b</italic>-constructs allowed the introduction of spin labels and the determination of intersubunit distances by ESR. These distances correlated well with molecular modeling results and strongly suggest that the <italic>E. coli</italic> subunit <italic>b</italic>-dimer can stably exist as a left-handed coiled-coil.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Delannoy, Sabine" sort="Delannoy, Sabine" uniqKey="Delannoy S" first="Sabine" last="Delannoy">Sabine Delannoy</name><name sortKey="Hornung, Tassilo" sort="Hornung, Tassilo" uniqKey="Hornung T" first="Tassilo" last="Hornung">Tassilo Hornung</name><name sortKey="Vogel, Pia D" sort="Vogel, Pia D" uniqKey="Vogel P" first="Pia D." last="Vogel">Pia D. Vogel</name><name sortKey="Volkov, Oleg A" sort="Volkov, Oleg A" uniqKey="Volkov O" first="Oleg A." last="Volkov">Oleg A. Volkov</name><name sortKey="Wise, John G" sort="Wise, John G" uniqKey="Wise J" first="John G." last="Wise">John G. Wise</name><name sortKey="Zaida, Tarek M A" sort="Zaida, Tarek M A" uniqKey="Zaida T" first="Tarek M. A." last="Zaida">Tarek M. A. 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