A new strategy employed for identification of sweet orange cultivars with RAPD markers.
Identifieur interne : 001418 ( Main/Exploration ); précédent : 001417; suivant : 001419A new strategy employed for identification of sweet orange cultivars with RAPD markers.
Auteurs : X. Sun [République populaire de Chine] ; Q. Mu ; D. Jiang ; C. Wang ; X C Wang ; J G FangSource :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Plant.
- chemical , metabolism : DNA Primers.
- classification : Citrus sinensis.
- genetics : Citrus sinensis.
- methods : Random Amplified Polymorphic DNA Technique.
- DNA Fingerprinting, Genetic Markers, Genotype.
Abstract
We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.
DOI: 10.4238/2012.August.6.11
PubMed: 22911591
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000586
- to stream PubMed, to step Curation: 000586
- to stream PubMed, to step Checkpoint: 000586
- to stream Ncbi, to step Merge: 001072
- to stream Ncbi, to step Curation: 001072
- to stream Ncbi, to step Checkpoint: 001072
- to stream Main, to step Merge: 001433
- to stream Main, to step Curation: 001418
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A new strategy employed for identification of sweet orange cultivars with RAPD markers.</title><author><name sortKey="Sun, X" sort="Sun, X" uniqKey="Sun X" first="X" last="Sun">X. Sun</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Mu, Q" sort="Mu, Q" uniqKey="Mu Q" first="Q" last="Mu">Q. Mu</name></author><author><name sortKey="Jiang, D" sort="Jiang, D" uniqKey="Jiang D" first="D" last="Jiang">D. Jiang</name></author><author><name sortKey="Wang, C" sort="Wang, C" uniqKey="Wang C" first="C" last="Wang">C. Wang</name></author><author><name sortKey="Wang, X C" sort="Wang, X C" uniqKey="Wang X" first="X C" last="Wang">X C Wang</name></author><author><name sortKey="Fang, J G" sort="Fang, J G" uniqKey="Fang J" first="J G" last="Fang">J G Fang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22911591</idno><idno type="pmid">22911591</idno><idno type="doi">10.4238/2012.August.6.11</idno><idno type="wicri:Area/PubMed/Corpus">000586</idno><idno type="wicri:Area/PubMed/Curation">000586</idno><idno type="wicri:Area/PubMed/Checkpoint">000586</idno><idno type="wicri:Area/Ncbi/Merge">001072</idno><idno type="wicri:Area/Ncbi/Curation">001072</idno><idno type="wicri:Area/Ncbi/Checkpoint">001072</idno><idno type="wicri:Area/Main/Merge">001433</idno><idno type="wicri:Area/Main/Curation">001418</idno><idno type="wicri:Area/Main/Exploration">001418</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new strategy employed for identification of sweet orange cultivars with RAPD markers.</title><author><name sortKey="Sun, X" sort="Sun, X" uniqKey="Sun X" first="X" last="Sun">X. Sun</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, Nanjing, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Mu, Q" sort="Mu, Q" uniqKey="Mu Q" first="Q" last="Mu">Q. Mu</name></author><author><name sortKey="Jiang, D" sort="Jiang, D" uniqKey="Jiang D" first="D" last="Jiang">D. Jiang</name></author><author><name sortKey="Wang, C" sort="Wang, C" uniqKey="Wang C" first="C" last="Wang">C. Wang</name></author><author><name sortKey="Wang, X C" sort="Wang, X C" uniqKey="Wang X" first="X C" last="Wang">X C Wang</name></author><author><name sortKey="Fang, J G" sort="Fang, J G" uniqKey="Fang J" first="J G" last="Fang">J G Fang</name></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (classification)</term><term>Citrus sinensis (genetics)</term><term>DNA Fingerprinting</term><term>DNA Primers (metabolism)</term><term>DNA, Plant (genetics)</term><term>Genetic Markers</term><term>Genotype</term><term>Random Amplified Polymorphic DNA Technique (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Fingerprinting</term><term>Genetic Markers</term><term>Genotype</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Fang, J G" sort="Fang, J G" uniqKey="Fang J" first="J G" last="Fang">J G Fang</name><name sortKey="Jiang, D" sort="Jiang, D" uniqKey="Jiang D" first="D" last="Jiang">D. Jiang</name><name sortKey="Mu, Q" sort="Mu, Q" uniqKey="Mu Q" first="Q" last="Mu">Q. Mu</name><name sortKey="Wang, C" sort="Wang, C" uniqKey="Wang C" first="C" last="Wang">C. Wang</name><name sortKey="Wang, X C" sort="Wang, X C" uniqKey="Wang X" first="X C" last="Wang">X C Wang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Sun, X" sort="Sun, X" uniqKey="Sun X" first="X" last="Sun">X. Sun</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001418 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001418 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22911591
|texte= A new strategy employed for identification of sweet orange cultivars with RAPD markers.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22911591" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |