Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance.
Identifieur interne : 001278 ( Main/Exploration ); précédent : 001277; suivant : 001279Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance.
Auteurs : M-Carmen Estevez [Espagne] ; Jose Belenguer ; Silvia Gomez-Montes ; Javier Miralles ; Alfonso M. Escuela ; Angel Montoya ; Laura M. LechugaSource :
- The Analyst [ 1364-5528 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Fungicides, Industrial, Pesticide Residues, Thiabendazole.
- analysis : Food Contamination.
- chemistry : Citrus sinensis.
- chemical , immunology : Thiabendazole.
- methods : Biosensing Techniques, Immunoassay.
- Surface Plasmon Resonance.
Abstract
A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 μg L(-1)) and an IC(50) of 3.2 nM (0.64 μg L(-1)) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.
DOI: 10.1039/c2an36094b
PubMed: 23057069
Affiliations:
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Le document en format XML
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Escuela</name></author><author><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Angel" last="Montoya">Angel Montoya</name></author><author><name sortKey="Lechuga, Laura M" sort="Lechuga, Laura M" uniqKey="Lechuga L" first="Laura M" last="Lechuga">Laura M. 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Escuela</name></author><author><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Angel" last="Montoya">Angel Montoya</name></author><author><name sortKey="Lechuga, Laura M" sort="Lechuga, Laura M" uniqKey="Lechuga L" first="Laura M" last="Lechuga">Laura M. 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An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 μg L(-1)) and an IC(50) of 3.2 nM (0.64 μg L(-1)) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region><settlement><li>Barcelone</li></settlement></list><tree><noCountry><name sortKey="Belenguer, Jose" sort="Belenguer, Jose" uniqKey="Belenguer J" first="Jose" last="Belenguer">Jose Belenguer</name><name sortKey="Escuela, Alfonso M" sort="Escuela, Alfonso M" uniqKey="Escuela A" first="Alfonso M" last="Escuela">Alfonso M. Escuela</name><name sortKey="Gomez Montes, Silvia" sort="Gomez Montes, Silvia" uniqKey="Gomez Montes S" first="Silvia" last="Gomez-Montes">Silvia Gomez-Montes</name><name sortKey="Lechuga, Laura M" sort="Lechuga, Laura M" uniqKey="Lechuga L" first="Laura M" last="Lechuga">Laura M. Lechuga</name><name sortKey="Miralles, Javier" sort="Miralles, Javier" uniqKey="Miralles J" first="Javier" last="Miralles">Javier Miralles</name><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Angel" last="Montoya">Angel Montoya</name></noCountry><country name="Espagne"><region name="Catalogne"><name sortKey="Estevez, M Carmen" sort="Estevez, M Carmen" uniqKey="Estevez M" first="M-Carmen" last="Estevez">M-Carmen Estevez</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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