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Production of polygalacturonases by Aspergillus section Nigri strains in a fixed bed reactor.

Identifieur interne : 000D50 ( Main/Exploration ); précédent : 000D49; suivant : 000D51

Production of polygalacturonases by Aspergillus section Nigri strains in a fixed bed reactor.

Auteurs : Marília Maciel [Brésil] ; Cristiane Ottoni ; Cledir Santos ; Nelson Lima ; Keila Moreira ; Cristina Souza-Motta

Source :

Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2013.

RBID : pubmed:23358324

English descriptors

KwdEn :
- Aspergillus (cytology), Aspergillus (enzymology), Aspergillus (growth & development), Aspergillus (ultrastructure), Biomass, Bioreactors (microbiology), Citrus sinensis, Enzyme Assays, Fermentation, Polygalacturonase (biosynthesis).
MESH :
- chemical , biosynthesis : Polygalacturonase.
- cytology : Aspergillus.
- enzymology : Aspergillus.
- growth & development : Aspergillus.
- microbiology : Bioreactors.
- ultrastructure : Aspergillus.
- Biomass, Citrus sinensis, Enzyme Assays, Fermentation.

Abstract

Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U · mL-1 and 4.11 U · mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level.

DOI: 10.3390/molecules18021660
PubMed: 23358324

Affiliations:

Le document en format XML

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<author><name sortKey="Souza Motta, Cristina" sort="Souza Motta, Cristina" uniqKey="Souza Motta C" first="Cristina" last="Souza-Motta">Cristina Souza-Motta</name>
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<author><name sortKey="Souza Motta, Cristina" sort="Souza Motta, Cristina" uniqKey="Souza Motta C" first="Cristina" last="Souza-Motta">Cristina Souza-Motta</name>
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<term>Biomass</term>
<term>Bioreactors (microbiology)</term>
<term>Citrus sinensis</term>
<term>Enzyme Assays</term>
<term>Fermentation</term>
<term>Polygalacturonase (biosynthesis)</term>
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<front><div type="abstract" xml:lang="en">Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U · mL-1 and 4.11 U · mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level.</div>
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<country name="Brésil"><region name="Pernambuco"><name sortKey="Maciel, Marilia" sort="Maciel, Marilia" uniqKey="Maciel M" first="Marília" last="Maciel">Marília Maciel</name>
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Production of polygalacturonases by Aspergillus section Nigri strains in a fixed bed reactor.

Production of polygalacturonases by Aspergillus section Nigri strains in a fixed bed reactor.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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