Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression
Identifieur interne : 000D22 ( Main/Exploration ); précédent : 000D21; suivant : 000D23Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression
Auteurs : Victor Aguilar-Hernández [Mexique] ; Plinio Guzmán [Mexique]Source :
- BMC Plant Biology [ 1471-2229 ] ; 2013.
Abstract
Introns located close to the 5′ end of a gene or in the 5′ untranslated region often exert positive effects on gene expression. This effect, known as intron-mediated enhancement (IME), has been observed in diverse eukaryotic organisms, including plants. The sequences involved in IME seem to be spread across the intron and function in an additive manner. The IMEter algorithm was developed to predict plant introns that may enhance gene expression. We have identified several plant members of the BTL class of E3s, which may have orthologs across eukaryotes, that contain a 5′UTR intron. The RING finger E3 ligases are key enzymes of the ubiquitination system that mediate the transfer of ubiquitin to substrates.
In this study, we retrieved
Analysis performed in two Arabidopsis
Url:
DOI: 10.1186/1471-2229-13-179
PubMed: 24228887
PubMed Central: 4225707
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression</title><author><name sortKey="Aguilar Hernandez, Victor" sort="Aguilar Hernandez, Victor" uniqKey="Aguilar Hernandez V" first="Victor" last="Aguilar-Hernández">Victor Aguilar-Hernández</name><affiliation wicri:level="1"><nlm:aff id="I1">Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821</wicri:regionArea><wicri:noRegion>Gto 36821</wicri:noRegion></affiliation></author><author><name sortKey="Guzman, Plinio" sort="Guzman, Plinio" uniqKey="Guzman P" first="Plinio" last="Guzmán">Plinio Guzmán</name><affiliation wicri:level="1"><nlm:aff id="I1">Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821</wicri:regionArea><wicri:noRegion>Gto 36821</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24228887</idno><idno type="pmc">4225707</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225707</idno><idno type="RBID">PMC:4225707</idno><idno type="doi">10.1186/1471-2229-13-179</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000B46</idno><idno type="wicri:Area/Pmc/Curation">000B45</idno><idno type="wicri:Area/Pmc/Checkpoint">000981</idno><idno type="wicri:Area/Ncbi/Merge">001492</idno><idno type="wicri:Area/Ncbi/Curation">001492</idno><idno type="wicri:Area/Ncbi/Checkpoint">001492</idno><idno type="wicri:Area/Main/Merge">000D26</idno><idno type="wicri:Area/Main/Curation">000D22</idno><idno type="wicri:Area/Main/Exploration">000D22</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression</title><author><name sortKey="Aguilar Hernandez, Victor" sort="Aguilar Hernandez, Victor" uniqKey="Aguilar Hernandez V" first="Victor" last="Aguilar-Hernández">Victor Aguilar-Hernández</name><affiliation wicri:level="1"><nlm:aff id="I1">Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821</wicri:regionArea><wicri:noRegion>Gto 36821</wicri:noRegion></affiliation></author><author><name sortKey="Guzman, Plinio" sort="Guzman, Plinio" uniqKey="Guzman P" first="Plinio" last="Guzmán">Plinio Guzmán</name><affiliation wicri:level="1"><nlm:aff id="I1">Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto 36821</wicri:regionArea><wicri:noRegion>Gto 36821</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Plant Biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Introns located close to the 5′ end of a gene or in the 5′ untranslated region often exert positive effects on gene expression. This effect, known as intron-mediated enhancement (IME), has been observed in diverse eukaryotic organisms, including plants. The sequences involved in IME seem to be spread across the intron and function in an additive manner. The IMEter algorithm was developed to predict plant introns that may enhance gene expression. We have identified several plant members of the BTL class of E3s, which may have orthologs across eukaryotes, that contain a 5′UTR intron. The RING finger E3 ligases are key enzymes of the ubiquitination system that mediate the transfer of ubiquitin to substrates.</p></sec><sec><title>Results</title><p>In this study, we retrieved <italic>BTL</italic> sequences from several angiosperm species and found that 5′UTR introns showing a strong IMEter score were predicted, suggesting that they may be conserved by lineage. Promoter-GUS fusion lines were used to confirm the IME effect of these 5′UTR introns on gene expression. IMEter scores of <italic>BTLs</italic> were compared with the 5′UTR introns of two gene families <italic>MHX</italic> and polyubiquitin genes.</p></sec><sec><title>Conclusions</title><p>Analysis performed in two Arabidopsis <italic>BTL</italic> E3 ligases genes indicated that the 5′UTR introns were essential for gene expression in all the tissues tested. Comparison of the average 5′UTR intron size on three gene families in ten angiosperm species suggests that a prevalent size for a 5′UTR intron is in the range of 600 nucleotides, and that the overall IMEter score within a gene family is preserved across several angiosperms. Our results indicated that gene expression dependent on a 5′UTR intron is an efficient regulatory mechanism in BTL E3 ligases that has been preserved throughout plant evolution.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Roy, Sw" uniqKey="Roy S">SW Roy</name></author><author><name sortKey="Gilbert, W" uniqKey="Gilbert W">W Gilbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mattick, Js" uniqKey="Mattick J">JS Mattick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Filichkin, Sa" uniqKey="Filichkin S">SA Filichkin</name></author><author><name sortKey="Priest, Hd" uniqKey="Priest H">HD Priest</name></author><author><name sortKey="Givan, Sa" uniqKey="Givan S">SA Givan</name></author><author><name sortKey="Shen, R" uniqKey="Shen R">R Shen</name></author><author><name sortKey="Bryant, Dw" uniqKey="Bryant D">DW Bryant</name></author><author><name 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name="Mexique"><noRegion><name sortKey="Aguilar Hernandez, Victor" sort="Aguilar Hernandez, Victor" uniqKey="Aguilar Hernandez V" first="Victor" last="Aguilar-Hernández">Victor Aguilar-Hernández</name></noRegion><name sortKey="Guzman, Plinio" sort="Guzman, Plinio" uniqKey="Guzman P" first="Plinio" last="Guzmán">Plinio Guzmán</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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