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In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

Identifieur interne : 000C33 ( Main/Exploration ); précédent : 000C32; suivant : 000C34

In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

Auteurs : Nizam Uddin ; Md. Rakib Hasan ; Md. Monir Hossain ; Arjyabrata Sarker ; A. H. M. Nazmul Hasan ; A. F. M. Mahmudul Islam ; Mohd. Motaher H. Chowdhury ; Md. Sohel Rana

Source :

Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2014.

RBID : PMC:3994357

Abstract

Objective

To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo).

Methods

Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.

Results

Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC₅₀ value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1 000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.

Conclusion

These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994357

DOI: 10.12980/APJTB.4.2014C1173
PubMed: 25182949
PubMed Central: 3994357

Affiliations:

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 α-amylase inhibitory activity and <italic>in vivo</italic>
 hypoglycemic effect of methanol extract of <italic>Citrus macroptera</italic>
 Montr. fruit</title>
<author><name sortKey="Uddin, Nizam" sort="Uddin, Nizam" uniqKey="Uddin N" first="Nizam" last="Uddin">Nizam Uddin</name>
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<author><name sortKey="Hasan, Md Rakib" sort="Hasan, Md Rakib" uniqKey="Hasan M" first="Md. Rakib" last="Hasan">Md. Rakib Hasan</name>
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<author><name sortKey="Hossain, Md Monir" sort="Hossain, Md Monir" uniqKey="Hossain M" first="Md. Monir" last="Hossain">Md. Monir Hossain</name>
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<author><name sortKey="Islam, A F M Mahmudul" sort="Islam, A F M Mahmudul" uniqKey="Islam A" first="A. F. M. Mahmudul" last="Islam">A. F. M. Mahmudul Islam</name>
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<author><name sortKey="Chowdhury, Mohd Motaher H" sort="Chowdhury, Mohd Motaher H" uniqKey="Chowdhury M" first="Mohd. Motaher H." last="Chowdhury">Mohd. Motaher H. Chowdhury</name>
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<author><name sortKey="Rana, Md Sohel" sort="Rana, Md Sohel" uniqKey="Rana M" first="Md. Sohel" last="Rana">Md. Sohel Rana</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vitro</italic>
 α-amylase inhibitory activity and <italic>in vivo</italic>
 hypoglycemic effect of methanol extract of <italic>Citrus macroptera</italic>
 Montr. fruit</title>
<author><name sortKey="Uddin, Nizam" sort="Uddin, Nizam" uniqKey="Uddin N" first="Nizam" last="Uddin">Nizam Uddin</name>
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<author><name sortKey="Hasan, Md Rakib" sort="Hasan, Md Rakib" uniqKey="Hasan M" first="Md. Rakib" last="Hasan">Md. Rakib Hasan</name>
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<author><name sortKey="Hossain, Md Monir" sort="Hossain, Md Monir" uniqKey="Hossain M" first="Md. Monir" last="Hossain">Md. Monir Hossain</name>
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<author><name sortKey="Hasan, A H M Nazmul" sort="Hasan, A H M Nazmul" uniqKey="Hasan A" first="A. H. M. Nazmul" last="Hasan">A. H. M. Nazmul Hasan</name>
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<author><name sortKey="Islam, A F M Mahmudul" sort="Islam, A F M Mahmudul" uniqKey="Islam A" first="A. F. M. Mahmudul" last="Islam">A. F. M. Mahmudul Islam</name>
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<author><name sortKey="Chowdhury, Mohd Motaher H" sort="Chowdhury, Mohd Motaher H" uniqKey="Chowdhury M" first="Mohd. Motaher H." last="Chowdhury">Mohd. Motaher H. Chowdhury</name>
</author>
<author><name sortKey="Rana, Md Sohel" sort="Rana, Md Sohel" uniqKey="Rana M" first="Md. Sohel" last="Rana">Md. Sohel Rana</name>
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<series><title level="j">Asian Pacific Journal of Tropical Biomedicine</title>
<idno type="ISSN">2221-1691</idno>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To investigate the therapeutic effects of methanol extract of <italic>Citrus macroptera</italic>
 Montr.fruit in α-amylase inhibitory activity (<italic>in vitro</italic>
) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (<italic>in vivo</italic>
).</p>
</sec>
<sec><title>Methods</title>
<p>Fruits of <italic>Citrus macroptera</italic>
 without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.</p>
</sec>
<sec><title>Results</title>
<p>Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC<sub>50</sub>
 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (<italic>P</italic>
<0.05 and <italic>P</italic>
<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (<italic>P</italic>
<0.05) at 2 h but 1 000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (<italic>P</italic>
<0.05 and <italic>P</italic>
<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with <italic>F</italic>
 value=23.83 and <italic>P</italic>
 value=0.0001 in oral glucose tolerance test.</p>
</sec>
<sec><title>Conclusion</title>
<p>These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.</p>
</sec>
</div>
</front>
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<name sortKey="Hasan, A H M Nazmul" sort="Hasan, A H M Nazmul" uniqKey="Hasan A" first="A. H. M. Nazmul" last="Hasan">A. H. M. Nazmul Hasan</name>
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<name sortKey="Islam, A F M Mahmudul" sort="Islam, A F M Mahmudul" uniqKey="Islam A" first="A. F. M. Mahmudul" last="Islam">A. F. M. Mahmudul Islam</name>
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<name sortKey="Uddin, Nizam" sort="Uddin, Nizam" uniqKey="Uddin N" first="Nizam" last="Uddin">Nizam Uddin</name>
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In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

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