Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: Quercus ilex L. subsp. ballota [Desf.] Samp.
Identifieur interne : 000530 ( Main/Exploration ); précédent : 000529; suivant : 000531Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: Quercus ilex L. subsp. ballota [Desf.] Samp.
Auteurs : M. Cristina Romero-Rodríguez [Espagne, Paraguay] ; Nieves Abril [Espagne] ; Rosa Sánchez-Lucas [Espagne] ; Jesús V. Jorrín-Novo [Espagne]Source :
- Frontiers in Plant Science [ 1664-462X ] ; 2015.
Abstract
As a preliminary step in the phosphoproteome analysis of germinating seeds (0 and 24 h after seed imbibition) and early grown seedlings (216 h after seed imbibition) from a non-orthodox sp.
Url:
DOI: 10.3389/fpls.2015.00620
PubMed: 26322061
PubMed Central: 4531236
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: <italic>Quercus ilex</italic> L. subsp. <italic>ballota</italic> [Desf.] Samp.</title><author><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M. Cristina" last="Romero-Rodríguez">M. Cristina Romero-Rodríguez</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Centro Multidisciplinario de Investigaciones Tecnológicas, Universidad Nacional de Asunción</institution><country>San Lorenzo, Paraguay</country></nlm:aff><country xml:lang="fr">Paraguay</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Abril, Nieves" sort="Abril, Nieves" uniqKey="Abril N" first="Nieves" last="Abril">Nieves Abril</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Sanchez Lucas, Rosa" sort="Sanchez Lucas, Rosa" uniqKey="Sanchez Lucas R" first="Rosa" last="Sánchez-Lucas">Rosa Sánchez-Lucas</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Jorrin Novo, Jesus V" sort="Jorrin Novo, Jesus V" uniqKey="Jorrin Novo J" first="Jesús V." last="Jorrín-Novo">Jesús V. Jorrín-Novo</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26322061</idno><idno type="pmc">4531236</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531236</idno><idno type="RBID">PMC:4531236</idno><idno type="doi">10.3389/fpls.2015.00620</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000078</idno><idno type="wicri:Area/Pmc/Curation">000078</idno><idno type="wicri:Area/Pmc/Checkpoint">000415</idno><idno type="wicri:Area/Ncbi/Merge">001C92</idno><idno type="wicri:Area/Ncbi/Curation">001C92</idno><idno type="wicri:Area/Ncbi/Checkpoint">001C92</idno><idno type="wicri:Area/Main/Merge">000530</idno><idno type="wicri:Area/Main/Curation">000530</idno><idno type="wicri:Area/Main/Exploration">000530</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: <italic>Quercus ilex</italic> L. subsp. <italic>ballota</italic> [Desf.] Samp.</title><author><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M. Cristina" last="Romero-Rodríguez">M. Cristina Romero-Rodríguez</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Centro Multidisciplinario de Investigaciones Tecnológicas, Universidad Nacional de Asunción</institution><country>San Lorenzo, Paraguay</country></nlm:aff><country xml:lang="fr">Paraguay</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Abril, Nieves" sort="Abril, Nieves" uniqKey="Abril N" first="Nieves" last="Abril">Nieves Abril</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Sanchez Lucas, Rosa" sort="Sanchez Lucas, Rosa" uniqKey="Sanchez Lucas R" first="Rosa" last="Sánchez-Lucas">Rosa Sánchez-Lucas</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Jorrin Novo, Jesus V" sort="Jorrin Novo, Jesus V" uniqKey="Jorrin Novo J" first="Jesús V." last="Jorrín-Novo">Jesús V. Jorrín-Novo</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Biochemistry and Molecular Biology, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Agricultural and Plant Proteomics Research Group, Department of Biochemistry and Molecular Biology, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, University of Cordoba</institution><country>Cordoba, Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea></wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Plant Science</title><idno type="eISSN">1664-462X</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>As a preliminary step in the phosphoproteome analysis of germinating seeds (0 and 24 h after seed imbibition) and early grown seedlings (216 h after seed imbibition) from a non-orthodox sp. <italic>Quercus ilex</italic>, a multiplex (SYPRO-Ruby and Pro-Q DPS) staining of high-resolution 2-DE gels was used. By using this protocol it was possible to detect changes in protein-abundance and/or phosphorylation status. This simple approach could be a good complementary alternative to the enrichment protocols used in the search for phosphoprotein candidates. While 482 spots were visualized with SYPRO-Ruby, 222 were with Pro-Q DPS. Statistically significant differences in spot intensity were observed among samples, these corresponding to 85 SYPRO-Ruby-, 20 Pro-Q-DPS-, and 35 SYPRO-Ruby and Pro-Q-DPS-stained spots. Fifty-five phosphoprotein candidates showing qualitative or quantitative differences between samples were subjected to MALDI-TOF-TOF MS analysis, with 20 of them being identified. Identified proteins belonged to five different functional categories, namely: carbohydrate and amino acid metabolism, defense, protein folding, and oxidation-reduction processes. With the exception of a putative cyclase, the other 19 proteins had at least one orthologous phosphoprotein in <italic>Arabidopsis thaliana, Medicago truncatula, N. tabacum</italic>, and <italic>Glycine max</italic>. Out of the 20 identified, seven showed differences in intensity in Pro-Q-DPS but not in SYPRO-Ruby-stained gels, including enzymes of the glycolysis and amino acid metabolism. This bears out that theory the regulation of these enzymes occurs at the post-translational level by phosphorylation with no changes at the transcriptional or translational level. This is different from the mechanism reported in orthodox seeds, in which concomitant changes in abundance and phosphorylation status have been observed for these enzymes.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agrawal, G K" uniqKey="Agrawal G">G. K. Agrawal</name></author><author><name sortKey="Thelen, J J" uniqKey="Thelen J">J. J. Thelen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agrawal, G K" uniqKey="Agrawal G">G. K. Agrawal</name></author><author><name sortKey="Thelen, J J" uniqKey="Thelen J">J. J. Thelen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alonso, J" uniqKey="Alonso J">J. Alonso</name></author><author><name sortKey="Zapata, C" uniqKey="Zapata C">C. Zapata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balbuena, T S" uniqKey="Balbuena T">T. S. Balbuena</name></author><author><name sortKey="Jo, L" uniqKey="Jo L">L. Jo</name></author><author><name sortKey="Pieruzzi, F P" uniqKey="Pieruzzi F">F. P. Pieruzzi</name></author><author><name sortKey="Dias, L L C" uniqKey="Dias L">L. L. C. Dias</name></author><author><name sortKey="Silveira, V" uniqKey="Silveira V">V. Silveira</name></author><author><name sortKey="Santa Catarina, C" uniqKey="Santa Catarina C">C. Santa-Catarina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bao, J" uniqKey="Bao J">J. Bao</name></author><author><name sortKey="Cheung, W Y" uniqKey="Cheung W">W. Y. Cheung</name></author><author><name sortKey="Jy, W" uniqKey="Jy W">W Jy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berggren, K" uniqKey="Berggren K">K. Berggren</name></author><author><name sortKey="Chernokalskaya, E" uniqKey="Chernokalskaya E">E. Chernokalskaya</name></author><author><name sortKey="Steinberg, T H" uniqKey="Steinberg T">T. H. Steinberg</name></author><author><name sortKey="Kemper, C" uniqKey="Kemper C">C. Kemper</name></author><author><name sortKey="Lopez, M F" uniqKey="Lopez M">M. F. Lopez</name></author><author><name sortKey="Diwu, Z" uniqKey="Diwu Z">Z. Diwu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bond, A E" uniqKey="Bond A">A. E. Bond</name></author><author><name sortKey="Row, P E" uniqKey="Row P">P. E. Row</name></author><author><name sortKey="Dudley, E" uniqKey="Dudley E">E. Dudley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bradford, M M" uniqKey="Bradford M">M. M. Bradford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, F" uniqKey="Chen F">F. Chen</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Zheng, J" uniqKey="Zheng J">J. Zheng</name></author><author><name sortKey="Huang, R" uniqKey="Huang R">R. Huang</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Hong, Z" uniqKey="Hong Z">Z. Hong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conesa, A" uniqKey="Conesa A">A. Conesa</name></author><author><name sortKey="Gotz, S" uniqKey="Gotz S">S. Götz</name></author><author><name sortKey="Garcia G Mez, J M" uniqKey="Garcia G Mez J">J. M. García-Gómez</name></author><author><name sortKey="Terol, J" uniqKey="Terol J">J. Terol</name></author><author><name sortKey="Tal N, M" uniqKey="Tal N M">M. Talón</name></author><author><name sortKey="Robles, M" uniqKey="Robles M">M. Robles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cutler, S R" uniqKey="Cutler S">S. R. Cutler</name></author><author><name sortKey="Rodriguez, P L" uniqKey="Rodriguez P">P. L. Rodriguez</name></author><author><name sortKey="Finkelstein, R R" uniqKey="Finkelstein R">R. R. Finkelstein</name></author><author><name sortKey="Abrams, S R" uniqKey="Abrams S">S. R. Abrams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fila, J" uniqKey="Fila J">J. Fíla</name></author><author><name sortKey="Matros, A" uniqKey="Matros A">A. Matros</name></author><author><name sortKey="Radau, S" uniqKey="Radau S">S. Radau</name></author><author><name sortKey="Zahedi, R P" uniqKey="Zahedi R">R. P. Zahedi</name></author><author><name sortKey="C Apkova, V" uniqKey="C Apkova V">V. C̀apková</name></author><author><name sortKey="Mock, H P" uniqKey="Mock H">H.-P. Mock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fujii, H" uniqKey="Fujii H">H. Fujii</name></author><author><name sortKey="Chinnusamy, V" uniqKey="Chinnusamy V">V. Chinnusamy</name></author><author><name sortKey="Rodrigues, A" uniqKey="Rodrigues A">A. Rodrigues</name></author><author><name sortKey="Rubio, S" uniqKey="Rubio S">S. Rubio</name></author><author><name sortKey="Antoni, R" uniqKey="Antoni R">R. Antoni</name></author><author><name sortKey="Park, S Y" uniqKey="Park S">S.-Y. Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gallego, F J" uniqKey="Gallego F">F. J. Gallego</name></author><author><name sortKey="De Algaba, A P" uniqKey="De Algaba A">A. P. De Algaba</name></author><author><name sortKey="Fernandez Escobar, R" uniqKey="Fernandez Escobar R">R. Fernandez-Escobar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, J" uniqKey="Gao J">J. Gao</name></author><author><name sortKey="Agrawal, G K" uniqKey="Agrawal G">G. K. Agrawal</name></author><author><name sortKey="Thelen, J J" uniqKey="Thelen J">J. J. Thelen</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorg, A" uniqKey="Gorg A">A. Görg</name></author><author><name sortKey="Weiss, W" uniqKey="Weiss W">W. Weiss</name></author><author><name sortKey="Dunn, M J" uniqKey="Dunn M">M. J. Dunn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grimsrud, P A" uniqKey="Grimsrud P">P. A. Grimsrud</name></author><author><name sortKey="Den Os, D" uniqKey="Den Os D">D. Den Os</name></author><author><name sortKey="Wenger, C D" uniqKey="Wenger C">C. D. Wenger</name></author><author><name sortKey="Swaney, D L" uniqKey="Swaney D">D. L. Swaney</name></author><author><name sortKey="Schwartz, D" uniqKey="Schwartz D">D. Schwartz</name></author><author><name sortKey="Sussman, M R" uniqKey="Sussman M">M. R. Sussman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, C" uniqKey="Han C">C. Han</name></author><author><name sortKey="Wang, K" uniqKey="Wang K">K. Wang</name></author><author><name sortKey="Yang, P" uniqKey="Yang P">P. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hunt, L" uniqKey="Hunt L">L. Hunt</name></author><author><name sortKey="Holdsworth, M J" uniqKey="Holdsworth M">M. J. Holdsworth</name></author><author><name sortKey="Gray, J E" uniqKey="Gray J">J. E. Gray</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones, A M E" uniqKey="Jones A">A. M. E. Jones</name></author><author><name sortKey="Maclean, D" uniqKey="Maclean D">D. Maclean</name></author><author><name sortKey="Studholme, D J" uniqKey="Studholme D">D. J. Studholme</name></author><author><name sortKey="Serna Sanz, A" uniqKey="Serna Sanz A">A. Serna-Sanz</name></author><author><name sortKey="Andreasson, E" uniqKey="Andreasson E">E. Andreasson</name></author><author><name sortKey="Rathjen, J P" uniqKey="Rathjen J">J. P. Rathjen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jorge, I" uniqKey="Jorge I">I. Jorge</name></author><author><name sortKey="Navarro, R M" uniqKey="Navarro R">R. M. Navarro</name></author><author><name sortKey="Lenz, C" uniqKey="Lenz C">C. Lenz</name></author><author><name sortKey="Ariza, D" uniqKey="Ariza D">D. Ariza</name></author><author><name sortKey="Jorrin, J" uniqKey="Jorrin J">J. Jorrín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaufmann, H" uniqKey="Kaufmann H">H. Kaufmann</name></author><author><name sortKey="Bailey, J E" uniqKey="Bailey J">J. E. Bailey</name></author><author><name sortKey="Fussenegger, M" uniqKey="Fussenegger M">M. Fussenegger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kersten, B" uniqKey="Kersten B">B. Kersten</name></author><author><name sortKey="Agrawal, G K" uniqKey="Agrawal G">G. K. Agrawal</name></author><author><name sortKey="Durek, P" uniqKey="Durek P">P. Durek</name></author><author><name sortKey="Neigenfind, J" uniqKey="Neigenfind J">J. Neigenfind</name></author><author><name sortKey="Schulze, W" uniqKey="Schulze W">W. Schulze</name></author><author><name sortKey="Walther, D" uniqKey="Walther D">D. Walther</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kersten, B" uniqKey="Kersten B">B. Kersten</name></author><author><name sortKey="Agrawal, G K" uniqKey="Agrawal G">G. K. Agrawal</name></author><author><name sortKey="Iwahashi, H" uniqKey="Iwahashi H">H. Iwahashi</name></author><author><name sortKey="Rakwal, R" uniqKey="Rakwal R">R. Rakwal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laemmli, U K" uniqKey="Laemmli U">U. K. Laemmli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leitner, A" uniqKey="Leitner A">A. Leitner</name></author><author><name sortKey="Sturm, M" uniqKey="Sturm M">M. Sturm</name></author><author><name sortKey="Lindner, W" uniqKey="Lindner W">W. Lindner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, H" uniqKey="Li H">H. Li</name></author><author><name sortKey="Wong, W S" uniqKey="Wong W">W. S. Wong</name></author><author><name sortKey="Zhu, L" uniqKey="Zhu L">L. Zhu</name></author><author><name sortKey="Guo, H W" uniqKey="Guo H">H. W. Guo</name></author><author><name sortKey="Ecker, J" uniqKey="Ecker J">J. Ecker</name></author><author><name sortKey="Li, N" uniqKey="Li N">N. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Silva Sanchez, C" uniqKey="Silva Sanchez C">C. Silva-Sanchez</name></author><author><name sortKey="Zhang, T" uniqKey="Zhang T">T. Zhang</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S. Chen</name></author><author><name sortKey="Li, H" uniqKey="Li H">H. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Zhuo, J" uniqKey="Zhuo J">J. Zhuo</name></author><author><name sortKey="Jing, Y" uniqKey="Jing Y">Y. Jing</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Liu, G" uniqKey="Liu G">G. Liu</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q. Li</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Hou, L" uniqKey="Hou L">L. Hou</name></author><author><name sortKey="Li, G" uniqKey="Li G">G. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, T C" uniqKey="Lu T">T.-C. Lu</name></author><author><name sortKey="Meng, L B" uniqKey="Meng L">L.-B. Meng</name></author><author><name sortKey="Yang, C P" uniqKey="Yang C">C.-P. Yang</name></author><author><name sortKey="Liu, G F" uniqKey="Liu G">G.-F. Liu</name></author><author><name sortKey="Liu, G J" uniqKey="Liu G">G.-J. Liu</name></author><author><name sortKey="Ma, W" uniqKey="Ma W">W. Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maldonado, A M" uniqKey="Maldonado A">A. M. Maldonado</name></author><author><name sortKey="Echevarria Zome O, S" uniqKey="Echevarria Zome O S">S. Echevarría-Zomeño</name></author><author><name sortKey="Jean Baptiste, S" uniqKey="Jean Baptiste S">S. Jean-Baptiste</name></author><author><name sortKey="Hernandez, M" uniqKey="Hernandez M">M. Hernández</name></author><author><name sortKey="Jorrin Novo, J V" uniqKey="Jorrin Novo J">J. V. Jorrín-Novo</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, L J" uniqKey="Meyer L">L. J. Meyer</name></author><author><name sortKey="Gao, J" uniqKey="Gao J">J. Gao</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D. Xu</name></author><author><name sortKey="Thelen, J J" uniqKey="Thelen J">J. J. Thelen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakagami, H" uniqKey="Nakagami H">H. Nakagami</name></author><author><name sortKey="Sugiyama, N" uniqKey="Sugiyama N">N. Sugiyama</name></author><author><name sortKey="Mochida, K" uniqKey="Mochida K">K. Mochida</name></author><author><name sortKey="Daudi, A" uniqKey="Daudi A">A. Daudi</name></author><author><name sortKey="Yoshida, Y" uniqKey="Yoshida Y">Y. Yoshida</name></author><author><name sortKey="Toyoda, T" uniqKey="Toyoda T">T. Toyoda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pulido, F J" uniqKey="Pulido F">F. J. Pulido</name></author><author><name sortKey="Diaz, M" uniqKey="Diaz M">M. DíAz</name></author><author><name sortKey="Hidalgo De Trucios, S J" uniqKey="Hidalgo De Trucios S">S. J. Hidalgo De Trucios</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reiland, S" uniqKey="Reiland S">S. Reiland</name></author><author><name sortKey="Messerli, G" uniqKey="Messerli G">G. Messerli</name></author><author><name sortKey="Baerenfaller, K" uniqKey="Baerenfaller K">K. Baerenfaller</name></author><author><name sortKey="Gerrits, B" uniqKey="Gerrits B">B. Gerrits</name></author><author><name sortKey="Endler, A" uniqKey="Endler A">A. Endler</name></author><author><name sortKey="Grossmann, J" uniqKey="Grossmann J">J. Grossmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rigbolt, K T G" uniqKey="Rigbolt K">K. T. G. Rigbolt</name></author><author><name sortKey="Blagoev, B" uniqKey="Blagoev B">B. Blagoev</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romero Rodriguez, M C" uniqKey="Romero Rodriguez M">M. C. Romero-Rodríguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romero Rodriguez, M C" uniqKey="Romero Rodriguez M">M. C. Romero-Rodriguez</name></author><author><name sortKey="Pascual, J" uniqKey="Pascual J">J. Pascual</name></author><author><name sortKey="Valledor, L" uniqKey="Valledor L">L. Valledor</name></author><author><name sortKey="Jorrin Novo, J" uniqKey="Jorrin Novo J">J. Jorrin-Novo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rose, C M" uniqKey="Rose C">C. M. Rose</name></author><author><name sortKey="Venkateshwaran, M" uniqKey="Venkateshwaran M">M. Venkateshwaran</name></author><author><name sortKey="Grimsrud, P A" uniqKey="Grimsrud P">P. A. Grimsrud</name></author><author><name sortKey="Westphall, M S" uniqKey="Westphall M">M. S. Westphall</name></author><author><name sortKey="Sussman, M R" uniqKey="Sussman M">M. R. Sussman</name></author><author><name sortKey="Coon, J J" uniqKey="Coon J">J. J. Coon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rose, C M" uniqKey="Rose C">C. M. Rose</name></author><author><name sortKey="Venkateshwaran, M" uniqKey="Venkateshwaran M">M. Venkateshwaran</name></author><author><name sortKey="Volkening, J D" uniqKey="Volkening J">J. D. Volkening</name></author><author><name sortKey="Grimsrud, P A" uniqKey="Grimsrud P">P. A. Grimsrud</name></author><author><name sortKey="Maeda, J" uniqKey="Maeda J">J. Maeda</name></author><author><name sortKey="Bailey, D J" uniqKey="Bailey D">D. J. Bailey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sghaier Hammami, B" uniqKey="Sghaier Hammami B">B. Sghaier-Hammami</name></author><author><name sortKey="Valero Galvan, J" uniqKey="Valero Galvan J">J. Valero-Galvàn</name></author><author><name sortKey="Romero Rodriguez, M C" uniqKey="Romero Rodriguez M">M. C. Romero-Rodríguez</name></author><author><name sortKey="Navarro Cerrillo, R M" uniqKey="Navarro Cerrillo R">R. M. Navarro-Cerrillo</name></author><author><name sortKey="Abdelly, C" uniqKey="Abdelly C">C. Abdelly</name></author><author><name sortKey="Jorrin Novo, J" uniqKey="Jorrin Novo J">J. Jorrín-Novo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sharov, A A" uniqKey="Sharov A">A. A. Sharov</name></author><author><name sortKey="Dudekula, D B" uniqKey="Dudekula D">D. B. Dudekula</name></author><author><name sortKey="Ko, M S H" uniqKey="Ko M">M. S. H. Ko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shevchenko, A" uniqKey="Shevchenko A">A. Shevchenko</name></author><author><name sortKey="Wilm, M" uniqKey="Wilm M">M. Wilm</name></author><author><name sortKey="Vorm, O" uniqKey="Vorm O">O. Vorm</name></author><author><name sortKey="Mann, M" uniqKey="Mann M">M. Mann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subba, P" uniqKey="Subba P">P. Subba</name></author><author><name sortKey="Barua, P" uniqKey="Barua P">P. Barua</name></author><author><name sortKey="Kumar, R" uniqKey="Kumar R">R. Kumar</name></author><author><name sortKey="Datta, A" uniqKey="Datta A">A. Datta</name></author><author><name sortKey="Soni, K K" uniqKey="Soni K">K. K. Soni</name></author><author><name sortKey="Chakraborty, S" uniqKey="Chakraborty S">S. Chakraborty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sugiyama, N" uniqKey="Sugiyama N">N. Sugiyama</name></author><author><name sortKey="Nakagami, H" uniqKey="Nakagami H">H. Nakagami</name></author><author><name sortKey="Mochida, K" uniqKey="Mochida K">K. Mochida</name></author><author><name sortKey="Daudi, A" uniqKey="Daudi A">A. Daudi</name></author><author><name sortKey="Tomita, M" uniqKey="Tomita M">M. Tomita</name></author><author><name sortKey="Shirasu, K" uniqKey="Shirasu K">K. Shirasu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taji, T" uniqKey="Taji T">T. Taji</name></author><author><name sortKey="Ohsumi, C" uniqKey="Ohsumi C">C. Ohsumi</name></author><author><name sortKey="Iuchi, S" uniqKey="Iuchi S">S. Iuchi</name></author><author><name sortKey="Seki, M" uniqKey="Seki M">M. Seki</name></author><author><name sortKey="Kasuga, M" uniqKey="Kasuga M">M. Kasuga</name></author><author><name sortKey="Kobayashi, M" uniqKey="Kobayashi M">M. Kobayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thingholm, T E" uniqKey="Thingholm T">T. E. Thingholm</name></author><author><name sortKey="Jensen, O N" uniqKey="Jensen O">O. N. Jensen</name></author><author><name sortKey="Larsen, M R" uniqKey="Larsen M">M. R. Larsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Umezawa, T" uniqKey="Umezawa T">T. Umezawa</name></author><author><name sortKey="Sugiyama, N" uniqKey="Sugiyama N">N. Sugiyama</name></author><author><name sortKey="Takahashi, F" uniqKey="Takahashi F">F. Takahashi</name></author><author><name sortKey="Anderson, J C" uniqKey="Anderson J">J. C. Anderson</name></author><author><name sortKey="Ishihama, Y" uniqKey="Ishihama Y">Y. Ishihama</name></author><author><name sortKey="Peck, S C" uniqKey="Peck S">S. C. Peck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valero Galvan, J" uniqKey="Valero Galvan J">J. Valero Galván</name></author><author><name sortKey="Jorrin Novo, J" uniqKey="Jorrin Novo J">J. Jorrín Novo</name></author><author><name sortKey="Cabrera, A" uniqKey="Cabrera A">A. Cabrera</name></author><author><name sortKey="Ariza, D" uniqKey="Ariza D">D. Ariza</name></author><author><name sortKey="Garcia Olmo, J" uniqKey="Garcia Olmo J">J. García-Olmo</name></author><author><name sortKey="Cerrillo, R" uniqKey="Cerrillo R">R. Cerrillo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valero Galvan, J" uniqKey="Valero Galvan J">J. Valero Galván</name></author><author><name sortKey="Valledor, L" uniqKey="Valledor L">L. Valledor</name></author><author><name sortKey="Cerrillo, R M N" uniqKey="Cerrillo R">R. M. N. Cerrillo</name></author><author><name sortKey="Pelegrin, E G" uniqKey="Pelegrin E">E. G. Pelegrín</name></author><author><name sortKey="Jorrin Novo, J V" uniqKey="Jorrin Novo J">J. V. Jorrín-Novo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valero Galvan, J" uniqKey="Valero Galvan J">J. Valero Galván</name></author><author><name sortKey="Valledor, L" uniqKey="Valledor L">L. Valledor</name></author><author><name sortKey="Gonzalez Fernandez, R" uniqKey="Gonzalez Fernandez R">R. González Fernandez</name></author><author><name sortKey="Navarro Cerrillo, R M" uniqKey="Navarro Cerrillo R">R. M. Navarro Cerrillo</name></author><author><name sortKey="Jorrin Novo, J V" uniqKey="Jorrin Novo J">J. V. Jorrín-Novo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valledor, L" uniqKey="Valledor L">L. Valledor</name></author><author><name sortKey="Jorrin, J" uniqKey="Jorrin J">J. Jorrín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walters, C" uniqKey="Walters C">C. Walters</name></author><author><name sortKey="Berjak, P" uniqKey="Berjak P">P. Berjak</name></author><author><name sortKey="Pammenter, N" uniqKey="Pammenter N">N. Pammenter</name></author><author><name sortKey="Kennedy, K" uniqKey="Kennedy K">K. Kennedy</name></author><author><name sortKey="Raven, P" uniqKey="Raven P">P. Raven</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Vignani, R" uniqKey="Vignani R">R. Vignani</name></author><author><name sortKey="Scali, M" uniqKey="Scali M">M. Scali</name></author><author><name sortKey="Cresti, M" uniqKey="Cresti M">M. Cresti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woods Ignatoski, K" uniqKey="Woods Ignatoski K">K. Woods Ignatoski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, R" uniqKey="Wu R">R. Wu</name></author><author><name sortKey="Haas, W" uniqKey="Haas W">W. Haas</name></author><author><name sortKey="Dephoure, N" uniqKey="Dephoure N">N. Dephoure</name></author><author><name sortKey="Huttlin, E L" uniqKey="Huttlin E">E. L. Huttlin</name></author><author><name sortKey="Zhai, B" uniqKey="Zhai B">B. Zhai</name></author><author><name sortKey="Sowa, M E" uniqKey="Sowa M">M. E. Sowa</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li><li>Paraguay</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M. Cristina" last="Romero-Rodríguez">M. Cristina Romero-Rodríguez</name></noRegion><name sortKey="Abril, Nieves" sort="Abril, Nieves" uniqKey="Abril N" first="Nieves" last="Abril">Nieves Abril</name><name sortKey="Jorrin Novo, Jesus V" sort="Jorrin Novo, Jesus V" uniqKey="Jorrin Novo J" first="Jesús V." last="Jorrín-Novo">Jesús V. Jorrín-Novo</name><name sortKey="Jorrin Novo, Jesus V" sort="Jorrin Novo, Jesus V" uniqKey="Jorrin Novo J" first="Jesús V." last="Jorrín-Novo">Jesús V. Jorrín-Novo</name><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M. Cristina" last="Romero-Rodríguez">M. Cristina Romero-Rodríguez</name><name sortKey="Sanchez Lucas, Rosa" sort="Sanchez Lucas, Rosa" uniqKey="Sanchez Lucas R" first="Rosa" last="Sánchez-Lucas">Rosa Sánchez-Lucas</name><name sortKey="Sanchez Lucas, Rosa" sort="Sanchez Lucas, Rosa" uniqKey="Sanchez Lucas R" first="Rosa" last="Sánchez-Lucas">Rosa Sánchez-Lucas</name></country><country name="Paraguay"><noRegion><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M. Cristina" last="Romero-Rodríguez">M. Cristina Romero-Rodríguez</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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