Biosynthesis of allylic isoprenoid pyrophosphates by an enzyme preparation from the flavedo of Citrus paradisii
Identifieur interne : 004636 ( Main/Curation ); précédent : 004635; suivant : 004637Biosynthesis of allylic isoprenoid pyrophosphates by an enzyme preparation from the flavedo of Citrus paradisii
Auteurs : Luz M. Pérez [Colombie] ; Ram N Lozada [Colombie] ; Osvaldo Cori [Colombie]Source :
- Phytochemistry [ 0031-9422 ] ; 1983.
Abstract
A cell-free system obtained from Citrus paradisii flavedo transformed mevalonic acid into mono- and sesquiterpenoids of E- and Z-conformation. The enzyme system required bivalent metal ions and the presence of sulfhydryl groups. IPP isomerase activity (EC 5.3.3.2) was independent of metal ions and almost insensitive to sulfhydryl group reagents, while prenyltransferase (EC 2.5.1.1) was inactivated by DTNB and required bivalent metals for activity. The nature of the metal ion defined the stereochemistry of the products formed by prenyltransferase. The ratio of E–Z farnesylpyrophosphates was 3:1. This Citrus species could, therefore, be a good starting material for the study of the stereochemistry of the enzymes forming E and Z sesquiterpenoids.
Url:
DOI: 10.1016/0031-9422(83)83018-0
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000C72
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000C72
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001751
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004A75
Links to Exploration step
ISTEX:312FD29C5C52835DC0DA70BAE520F86DED917C3BLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Biosynthesis of allylic isoprenoid pyrophosphates by an enzyme preparation from the flavedo of Citrus paradisii</title>
<author><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. Pérez</name>
</author>
<author><name sortKey="Lozada, Ram N" sort="Lozada, Ram N" uniqKey="Lozada R" first="Ram N" last="Lozada">Ram N Lozada</name>
</author>
<author><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:312FD29C5C52835DC0DA70BAE520F86DED917C3B</idno>
<date when="1983" year="1983">1983</date>
<idno type="doi">10.1016/0031-9422(83)83018-0</idno>
<idno type="url">https://api.istex.fr/document/312FD29C5C52835DC0DA70BAE520F86DED917C3B/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C72</idno>
<idno type="wicri:Area/Istex/Curation">000C72</idno>
<idno type="wicri:Area/Istex/Checkpoint">001751</idno>
<idno type="wicri:doubleKey">0031-9422:1983:Perez L:biosynthesis:of:allylic</idno>
<idno type="wicri:Area/Main/Merge">004A75</idno>
<idno type="wicri:Area/Main/Curation">004636</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Biosynthesis of allylic isoprenoid pyrophosphates by an enzyme preparation from the flavedo of Citrus paradisii</title>
<author><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. Pérez</name>
<affiliation wicri:level="1"><country xml:lang="fr">Colombie</country>
<wicri:regionArea>Departmento de Química, Universidad de Antioquia, Medellín</wicri:regionArea>
<wicri:noRegion>Medellín</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lozada, Ram N" sort="Lozada, Ram N" uniqKey="Lozada R" first="Ram N" last="Lozada">Ram N Lozada</name>
<affiliation wicri:level="1"><country xml:lang="fr">Colombie</country>
<wicri:regionArea>Departmento de Química, Universidad de Antioquia, Medellín</wicri:regionArea>
<wicri:noRegion>Medellín</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name>
<affiliation wicri:level="1"><country xml:lang="fr">Colombie</country>
<wicri:regionArea>Departmento de Química, Universidad de Antioquia, Medellín</wicri:regionArea>
<wicri:noRegion>Medellín</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Phytochemistry</title>
<title level="j" type="abbrev">PHYTO</title>
<idno type="ISSN">0031-9422</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1983">1983</date>
<biblScope unit="volume">22</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="431">431</biblScope>
<biblScope unit="page" to="433">433</biblScope>
</imprint>
<idno type="ISSN">0031-9422</idno>
</series>
<idno type="istex">312FD29C5C52835DC0DA70BAE520F86DED917C3B</idno>
<idno type="DOI">10.1016/0031-9422(83)83018-0</idno>
<idno type="PII">0031-9422(83)83018-0</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0031-9422</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A cell-free system obtained from Citrus paradisii flavedo transformed mevalonic acid into mono- and sesquiterpenoids of E- and Z-conformation. The enzyme system required bivalent metal ions and the presence of sulfhydryl groups. IPP isomerase activity (EC 5.3.3.2) was independent of metal ions and almost insensitive to sulfhydryl group reagents, while prenyltransferase (EC 2.5.1.1) was inactivated by DTNB and required bivalent metals for activity. The nature of the metal ion defined the stereochemistry of the products formed by prenyltransferase. The ratio of E–Z farnesylpyrophosphates was 3:1. This Citrus species could, therefore, be a good starting material for the study of the stereochemistry of the enzymes forming E and Z sesquiterpenoids.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004636 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004636 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:312FD29C5C52835DC0DA70BAE520F86DED917C3B |texte= Biosynthesis of allylic isoprenoid pyrophosphates by an enzyme preparation from the flavedo of Citrus paradisii }}
This area was generated with Dilib version V0.6.25. |