Isolation and characterization of salt-associated protein in Citrus
Identifieur interne : 003788 ( Main/Curation ); précédent : 003787; suivant : 003789Isolation and characterization of salt-associated protein in Citrus
Auteurs : Gozal Ben-Hayyim [Israël] ; Zehava Faltin [Israël] ; Shimon Gepstein [Israël] ; Luc Camoin [France] ; A. Donny Strosberg [France] ; Yuval Eshdat [Israël]Source :
- Plant Science [ 0168-9452 ] ; 1993.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent olecular weight of 23–25 kDa and pI=6.1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinesis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties. Pulse labeling of the salt-tolerant cells suggests a low turnover of the accumulated protein. Its salt-induced accumulation in the cells was also relatively slow and gradually increased during a period of several days following exposure of the cells to NaCl. Cell fractionation experiments suggest that the protein is allocated in the soluble fraction and is not an integral membrane protein. Examination of the presence of the protein in citrus plants, such as Etrog citron (C. medicaL.) and Cleopatra mandarin (C. reticulata) cultivars, revealed an increase in its concentration in all organs examined, following irrigation of the plants with saline water. Thus, this protein is associated with stress conditions employed on growing plants as well and is not a unique phenomenon restricted to cultured cells only.
Url:
DOI: 10.1016/0168-9452(93)90084-D
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ISTEX:E62C7DE9F4E5580E2F6E39FFB07AD689D1394870Le document en format XML
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Donny Strosberg</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII and Institut Cochin de Génetique Moléculaire, 22 rue Méchain, 75014 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Friut Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="ISSN">0168-9452</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="129">129</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0168-9452</idno></series><idno type="istex">E62C7DE9F4E5580E2F6E39FFB07AD689D1394870</idno><idno type="DOI">10.1016/0168-9452(93)90084-D</idno><idno type="PII">0168-9452(93)90084-D</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell culture</term><term>Citrus sinensis</term><term>Gel electrophoresis</term><term>Gene expression</term><term>Osmolarity</term><term>Osmotic shock</term><term>Proteins</term><term>Salinity</term><term>Salt resistance</term><term>Stress</term><term>Tolerance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Choc osmotique</term><term>Citrus sinensis</term><term>Culture cellulaire</term><term>Electrophorèse gel</term><term>Expression génique</term><term>Osmolarité</term><term>Protéine</term><term>Résistance sel</term><term>Salinité</term><term>Stress</term><term>Tolérance</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent olecular weight of 23–25 kDa and pI=6.1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinesis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties. Pulse labeling of the salt-tolerant cells suggests a low turnover of the accumulated protein. Its salt-induced accumulation in the cells was also relatively slow and gradually increased during a period of several days following exposure of the cells to NaCl. Cell fractionation experiments suggest that the protein is allocated in the soluble fraction and is not an integral membrane protein. Examination of the presence of the protein in citrus plants, such as Etrog citron (C. medicaL.) and Cleopatra mandarin (C. reticulata) cultivars, revealed an increase in its concentration in all organs examined, following irrigation of the plants with saline water. Thus, this protein is associated with stress conditions employed on growing plants as well and is not a unique phenomenon restricted to cultured cells only.</div></front></TEI><double idat="0168-9452:1993:Ben Hayyim G:isolation:and:characterization"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Isolation and characterization of salt-associated protein in Citrus</title><author><name sortKey="Ben Hayyim, G" sort="Ben Hayyim, G" uniqKey="Ben Hayyim G" first="G." last="Ben-Hayyim">G. 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Gepstein</name></author><author><name sortKey="Camoin, L" sort="Camoin, L" uniqKey="Camoin L" first="L." last="Camoin">L. Camoin</name></author><author><name sortKey="Strosberg, A D" sort="Strosberg, A D" uniqKey="Strosberg A" first="A. D." last="Strosberg">A. D. Strosberg</name></author><author><name sortKey="Eshdat, Y" sort="Eshdat, Y" uniqKey="Eshdat Y" first="Y." last="Eshdat">Y. 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Ben-Hayyim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Volcani cent., agricultural res. organization, agricultural res. organization, dep. fruit tree breeding genetics</s1><s2>50250 Bet Dagan</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Faltin, Z" sort="Faltin, Z" uniqKey="Faltin Z" first="Z." last="Faltin">Z. Faltin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Volcani cent., agricultural res. organization, agricultural res. organization, dep. fruit tree breeding genetics</s1><s2>50250 Bet Dagan</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Gepstein, S" sort="Gepstein, S" uniqKey="Gepstein S" first="S." last="Gepstein">S. Gepstein</name></author><author><name sortKey="Camoin, L" sort="Camoin, L" uniqKey="Camoin L" first="L." last="Camoin">L. Camoin</name></author><author><name sortKey="Strosberg, A D" sort="Strosberg, A D" uniqKey="Strosberg A" first="A. D." last="Strosberg">A. D. Strosberg</name></author><author><name sortKey="Eshdat, Y" sort="Eshdat, Y" uniqKey="Eshdat Y" first="Y." last="Eshdat">Y. Eshdat</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Volcani cent., agricultural res. organization, agricultural res. organization, dep. fruit tree breeding genetics</s1><s2>50250 Bet Dagan</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant science : (Limerick)</title><title level="j" type="abbreviated">Plant sci. : (Limerick)</title><idno type="ISSN">0168-9452</idno><imprint><date when="1993">1993</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant science : (Limerick)</title><title level="j" type="abbreviated">Plant sci. : (Limerick)</title><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell culture</term><term>Citrus sinensis</term><term>Gel electrophoresis</term><term>Gene expression</term><term>Osmolarity</term><term>Osmotic shock</term><term>Proteins</term><term>Salinity</term><term>Salt resistance</term><term>Stress</term><term>Tolerance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Résistance sel</term><term>Expression génique</term><term>Tolérance</term><term>Choc osmotique</term><term>Salinité</term><term>Stress</term><term>Protéine</term><term>Culture cellulaire</term><term>Citrus sinensis</term><term>Electrophorèse gel</term><term>Osmolarité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular weight of 23-25 kDa and pI=6. 1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinensis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties</div></front></TEI></INIST><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Isolation and characterization of salt-associated protein in Citrus</title><author><name sortKey="Ben Hayyim, Gozal" sort="Ben Hayyim, Gozal" uniqKey="Ben Hayyim G" first="Gozal" last="Ben-Hayyim">Gozal Ben-Hayyim</name></author><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name></author><author><name sortKey="Gepstein, Shimon" sort="Gepstein, Shimon" uniqKey="Gepstein S" first="Shimon" last="Gepstein">Shimon Gepstein</name></author><author><name sortKey="Camoin, Luc" sort="Camoin, Luc" uniqKey="Camoin L" first="Luc" last="Camoin">Luc Camoin</name></author><author><name sortKey="Donny Strosberg, A" sort="Donny Strosberg, A" uniqKey="Donny Strosberg A" first="A." last="Donny Strosberg">A. Donny Strosberg</name></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E62C7DE9F4E5580E2F6E39FFB07AD689D1394870</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1016/0168-9452(93)90084-D</idno><idno type="url">https://api.istex.fr/document/E62C7DE9F4E5580E2F6E39FFB07AD689D1394870/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000636</idno><idno type="wicri:Area/Istex/Curation">000636</idno><idno type="wicri:Area/Istex/Checkpoint">000F99</idno><idno type="wicri:doubleKey">0168-9452:1993:Ben Hayyim G:isolation:and:characterization</idno><idno type="wicri:Area/Main/Merge">003A80</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Isolation and characterization of salt-associated protein in Citrus</title><author><name sortKey="Ben Hayyim, Gozal" sort="Ben Hayyim, Gozal" uniqKey="Ben Hayyim G" first="Gozal" last="Ben-Hayyim">Gozal Ben-Hayyim</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Friut Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Friut Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Gepstein, Shimon" sort="Gepstein, Shimon" uniqKey="Gepstein S" first="Shimon" last="Gepstein">Shimon Gepstein</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Faculty of Biology, The Technion, Haifa</wicri:regionArea><wicri:noRegion>Haifa</wicri:noRegion></affiliation></author><author><name sortKey="Camoin, Luc" sort="Camoin, Luc" uniqKey="Camoin L" first="Luc" last="Camoin">Luc Camoin</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII and Institut Cochin de Génetique Moléculaire, 22 rue Méchain, 75014 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Donny Strosberg, A" sort="Donny Strosberg, A" uniqKey="Donny Strosberg A" first="A." last="Donny Strosberg">A. Donny Strosberg</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII and Institut Cochin de Génetique Moléculaire, 22 rue Méchain, 75014 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Friut Tree Breeding and Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="ISSN">0168-9452</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="129">129</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0168-9452</idno></series><idno type="istex">E62C7DE9F4E5580E2F6E39FFB07AD689D1394870</idno><idno type="DOI">10.1016/0168-9452(93)90084-D</idno><idno type="PII">0168-9452(93)90084-D</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A significant increase in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent olecular weight of 23–25 kDa and pI=6.1, was observed in adapted salt-tolerant cultured cells derived from Shamuti orange (Citrus sinesis L. Osbeck) ovular callus cells. An increase was also determined when these cells were grown in the presence of abscisic acid (ABA) or polyethylene glycol (PEG) instead of NaCl, similarly to what was previously observed for osmotin, the tobacco salt-associated protein. However, no similarity has been so far observed between these proteins in their biochemical and immunochemical properties. Pulse labeling of the salt-tolerant cells suggests a low turnover of the accumulated protein. Its salt-induced accumulation in the cells was also relatively slow and gradually increased during a period of several days following exposure of the cells to NaCl. Cell fractionation experiments suggest that the protein is allocated in the soluble fraction and is not an integral membrane protein. Examination of the presence of the protein in citrus plants, such as Etrog citron (C. medicaL.) and Cleopatra mandarin (C. reticulata) cultivars, revealed an increase in its concentration in all organs examined, following irrigation of the plants with saline water. Thus, this protein is associated with stress conditions employed on growing plants as well and is not a unique phenomenon restricted to cultured cells only.</div></front></TEI></ISTEX></double></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Isolation and characterization of salt-associated protein in Citrus
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