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Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants

Identifieur interne : 003652 ( Main/Curation ); précédent : 003651; suivant : 003653

Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants

Auteurs : L. Pena [Espagne] ; M. Cervera ; J. Juarez ; A. Navarro ; J. A. Pina ; N. Duran-Vila ; L. Navarro

Source :

Plant cell reports [ 0721-7714 ] ; 1995.

RBID : Pascal:95-0427020

Descripteurs français

Pascal (Inist)
- Régénération, Transformation génétique, Agrobacterium tumefaciens, β-Glucuronidase, Plante transgénique, Culture tissu, Citrus sinensis, Kanamycin kinase, Expression génique, Végétal, Agrume, Gène.
Wicri :
- topic : Plante transgénique, Agrume.

English descriptors

KwdEn :
- Agrobacterium tumefaciens, Citrus fruit, Citrus sinensis, Gene expression, Genetic transformation, Kanamycin kinase, Regeneration, Tissue culture, Transgenic plant, Vegetals, β-Glucuronidase.

Abstract

Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.

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Pascal:95-0427020

Le document en format XML

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<author><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Inst. Valenciano investigaciones agrarias, dep. protección biotecnología</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
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<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
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<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
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<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
</author>
<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<publicationStmt><idno type="wicri:source">INIST</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants</title>
<author><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Inst. Valenciano investigaciones agrarias, dep. protección biotecnología</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
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<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
</author>
<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
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<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<series><title level="j" type="main">Plant cell reports</title>
<title level="j" type="abbreviated">Plant cell rep.</title>
<idno type="ISSN">0721-7714</idno>
<imprint><date when="1995">1995</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Gene expression</term>
<term>Genetic transformation</term>
<term>Kanamycin kinase</term>
<term>Regeneration</term>
<term>Tissue culture</term>
<term>Transgenic plant</term>
<term>Vegetals</term>
<term>β-Glucuronidase</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Régénération</term>
<term>Transformation génétique</term>
<term>Agrobacterium tumefaciens</term>
<term>β-Glucuronidase</term>
<term>Plante transgénique</term>
<term>Culture tissu</term>
<term>Citrus sinensis</term>
<term>Kanamycin kinase</term>
<term>Expression génique</term>
<term>Végétal</term>
<term>Agrume</term>
<term>Gène</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
<term>Agrume</term>
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<front><div type="abstract" xml:lang="en">Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.</div>
</front>
</TEI>
</record>

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Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants

Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri