Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants
Identifieur interne : 003652 ( Main/Curation ); précédent : 003651; suivant : 003653Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants
Auteurs : L. Pena [Espagne] ; M. Cervera ; J. Juarez ; A. Navarro ; J. A. Pina ; N. Duran-Vila ; L. NavarroSource :
- Plant cell reports [ 0721-7714 ] ; 1995.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Plante transgénique, Agrume.
English descriptors
- KwdEn :
Abstract
Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.
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Pascal:95-0427020Le document en format XML
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<author><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
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<s2>46113 Moncada</s2>
<s3>ESP</s3>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
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<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
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<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
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<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
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<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
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<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
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<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
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<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
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<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
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<series><title level="j" type="main">Plant cell reports</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Gene expression</term>
<term>Genetic transformation</term>
<term>Kanamycin kinase</term>
<term>Regeneration</term>
<term>Tissue culture</term>
<term>Transgenic plant</term>
<term>Vegetals</term>
<term>β-Glucuronidase</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Régénération</term>
<term>Transformation génétique</term>
<term>Agrobacterium tumefaciens</term>
<term>β-Glucuronidase</term>
<term>Plante transgénique</term>
<term>Culture tissu</term>
<term>Citrus sinensis</term>
<term>Kanamycin kinase</term>
<term>Expression génique</term>
<term>Végétal</term>
<term>Agrume</term>
<term>Gène</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
<term>Agrume</term>
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<front><div type="abstract" xml:lang="en">Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.</div>
</front>
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