Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange
Identifieur interne : 002D74 ( Main/Curation ); précédent : 002D73; suivant : 002D75Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange
Auteurs : J. M Moreira-Dias [Espagne] ; R. V Molina [Espagne] ; J. L Guardiola [Espagne] ; A. Garc A-Luis [Espagne]Source :
- Scientia Horticulturae [ 0304-4238 ] ; 2001.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- 1-Naphthaleneacetic acid, Adventitious shoots, Application method, Citrus sinensis×Poncirus trifoliata, Daylength, Direct organogenesis, Epicotyl, Growth, Growth regulators, Indirect organogenesis, Luminous intensity, Micropropagation, Morphogenesis, Organogenesis, Photon flux density, Photoperiod, Regeneration, Troyer citrange.
Abstract
The optimal growth regulator addenda for adventitious shoot regeneration in epicotyl cuttings of Troyer citrange (Citrus sinensis×Poncirus trifoliata) varied with the conditions of illumination. The response to illumination and to growth regulators differed for the direct and the indirect (through callusing) pathways of regeneration. Shoot formation through the direct organogenic pathway decreased as the concentration of benzyladenine (BA) in the medium was increased in the range 2.2–22μM, when the explants were incubated in the dark or under an 8h daylength. For explants incubated under a 16h daylength, the number of shoots formed increased with BA concentration. Optimal conditions of incubation for shoot formation through the direct pathway were either an 8h daylength with a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA, or a 16h daylength with a photon flux density of 37μmolm−2s−1 in the presence of 22μM BA. Irrespective of the conditions of incubation, shoot formation through the indirect organogenic pathway was suppressed by the addition of 22μM BA to the medium. Optimal conditions for shoot formation through this pathway were incubation under an 8h daylength at a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA. At the optimal conditions indicated, the addition of the synthetic auxin naphtaleneacetic acid (0.54μM) reduced shoot formation. Irrespective of the pathway of regeneration, the number of shoots formed decreased markedly with the distance of the cutting from the cotyledonary node.
Url:
DOI: 10.1016/S0304-4238(00)00186-2
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The response to illumination and to growth regulators differed for the direct and the indirect (through callusing) pathways of regeneration. Shoot formation through the direct organogenic pathway decreased as the concentration of benzyladenine (BA) in the medium was increased in the range 2.2–22μM, when the explants were incubated in the dark or under an 8h daylength. For explants incubated under a 16h daylength, the number of shoots formed increased with BA concentration. Optimal conditions of incubation for shoot formation through the direct pathway were either an 8h daylength with a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA, or a 16h daylength with a photon flux density of 37μmolm−2s−1 in the presence of 22μM BA. Irrespective of the conditions of incubation, shoot formation through the indirect organogenic pathway was suppressed by the addition of 22μM BA to the medium. Optimal conditions for shoot formation through this pathway were incubation under an 8h daylength at a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA. At the optimal conditions indicated, the addition of the synthetic auxin naphtaleneacetic acid (0.54μM) reduced shoot formation. Irrespective of the pathway of regeneration, the number of shoots formed decreased markedly with the distance of the cutting from the cotyledonary node.</div></front></TEI><double idat="0304-4238:2001:Moreira Dias J:daylength:and:photon"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange</title><author><name sortKey="Moreira Dias, J M" sort="Moreira Dias, J M" uniqKey="Moreira Dias J" first="J. M." last="Moreira-Dias">J. M. Moreira-Dias</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Molina, R V" sort="Molina, R V" uniqKey="Molina R" first="R. V." last="Molina">R. V. Molina</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Guardiola, J L" sort="Guardiola, J L" uniqKey="Guardiola J" first="J. L." last="Guardiola">J. L. Guardiola</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Garcia Luis, A" sort="Garcia Luis, A" uniqKey="Garcia Luis A" first="A." last="Garcia-Luis">A. Garcia-Luis</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">01-0124357</idno><date when="2001">2001</date><idno type="stanalyst">PASCAL 01-0124357 INIST</idno><idno type="RBID">Pascal:01-0124357</idno><idno type="wicri:Area/PascalFrancis/Corpus">000927</idno><idno type="wicri:Area/PascalFrancis/Curation">000077</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000890</idno><idno type="wicri:doubleKey">0304-4238:2001:Moreira Dias J:daylength:and:photon</idno><idno type="wicri:Area/Main/Merge">003046</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange</title><author><name sortKey="Moreira Dias, J M" sort="Moreira Dias, J M" uniqKey="Moreira Dias J" first="J. M." last="Moreira-Dias">J. M. Moreira-Dias</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Molina, R V" sort="Molina, R V" uniqKey="Molina R" first="R. V." last="Molina">R. V. Molina</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Guardiola, J L" sort="Guardiola, J L" uniqKey="Guardiola J" first="J. L." last="Guardiola">J. L. Guardiola</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Garcia Luis, A" sort="Garcia Luis, A" uniqKey="Garcia Luis A" first="A." last="Garcia-Luis">A. Garcia-Luis</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Departamento de Biología Vegetal, Universidad Politécnica de Valencia, PO Box 22012</s1><s2>46071 Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Scientia horticulturae</title><title level="j" type="abbreviated">Sci. hortic.</title><idno type="ISSN">0304-4238</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Scientia horticulturae</title><title level="j" type="abbreviated">Sci. hortic.</title><idno type="ISSN">0304-4238</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Naphthaleneacetic acid</term><term>Application method</term><term>Epicotyl</term><term>Growth</term><term>Luminous intensity</term><term>Micropropagation</term><term>Morphogenesis</term><term>Organogenesis</term><term>Photoperiod</term><term>Regeneration</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Morphogenèse</term><term>Epicotyle</term><term>Photopériode</term><term>Intensité lumineuse</term><term>Croissance</term><term>Multiplication végétative in vitro</term><term>Régénération</term><term>Organogenèse</term><term>Modalité traitement</term><term>Naphtalène-1-acétique acide</term><term>Citrus sinensis Poncirus trifoliata</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The optimal growth regulator addenda for adventitious shoot regeneration in epicotyl cuttings of Troyer citrange (Citrus sinensis × Poncirus trifoliata) varied with the conditions of illumination. The response to illumination and to growth regulators differed for the direct and the indirect (through callusing) pathways of regeneration. Shoot formation through the direct organogenic pathway decreased as the concentration of benzyladenine (BA) in the medium was increased in the range 2.2-22 μM, when the explants were incubated in the dark or under an 8 h daylength. For explants incubated under a 16 h daylength, the number of shoots formed increased with BA concentration. Optimal conditions of incubation for shoot formation through the direct pathway were either an 8 h daylength with a photon flux density of 74 μmol m<sup>-2</sup> s<sup>-1</sup> in the presence of 2.2 μM BA, or a 16 h daylength with a photon flux density of 37 μmol m<sup>-2</sup> s<sup>-1</sup> in the presence of 22 μM BA. Irrespective of the conditions of incubation, shoot formation through the indirect organogenic pathway was suppressed by the addition of 22 μM BA to the medium. Optimal conditions for shoot formation through this pathway were incubation under an 8 h daylength at a photon flux density of 74 μmol m <sup>2</sup> s<sup>-1</sup> in the presence of 2.2 μM BA. At the optimal conditions indicated, the addition of the synthetic auxin naphtaleneacetic acid (0.54 μM) reduced shoot formation. Irrespective of the pathway of regeneration, the number of shoots formed decreased markedly with the distance of the cutting from the cotyledonary node.</div></front></TEI></INIST><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange</title><author><name sortKey="Moreira Dias, J M" sort="Moreira Dias, J M" uniqKey="Moreira Dias J" first="J. M" last="Moreira-Dias">J. M Moreira-Dias</name></author><author><name sortKey="Molina, R V" sort="Molina, R V" uniqKey="Molina R" first="R. V" last="Molina">R. V Molina</name></author><author><name sortKey="Guardiola, J L" sort="Guardiola, J L" uniqKey="Guardiola J" first="J. L" last="Guardiola">J. L Guardiola</name></author><author><name sortKey="Garc A Luis, A" sort="Garc A Luis, A" uniqKey="Garc A Luis A" first="A" last="Garc A-Luis">A. Garc A-Luis</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C2490CE6B4DA818482D63532ED8C4BFA2D0D403F</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0304-4238(00)00186-2</idno><idno type="url">https://api.istex.fr/document/C2490CE6B4DA818482D63532ED8C4BFA2D0D403F/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000258</idno><idno type="wicri:Area/Istex/Curation">000258</idno><idno type="wicri:Area/Istex/Checkpoint">000984</idno><idno type="wicri:doubleKey">0304-4238:2001:Moreira Dias J:daylength:and:photon</idno><idno type="wicri:Area/Main/Merge">002F60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange</title><author><name sortKey="Moreira Dias, J M" sort="Moreira Dias, J M" uniqKey="Moreira Dias J" first="J. M" last="Moreira-Dias">J. M Moreira-Dias</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologı́a Vegetal, Universidad Politécnica de Valencia, PO Box 22012, 46071 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Molina, R V" sort="Molina, R V" uniqKey="Molina R" first="R. V" last="Molina">R. V Molina</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologı́a Vegetal, Universidad Politécnica de Valencia, PO Box 22012, 46071 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Guardiola, J L" sort="Guardiola, J L" uniqKey="Guardiola J" first="J. L" last="Guardiola">J. L Guardiola</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologı́a Vegetal, Universidad Politécnica de Valencia, PO Box 22012, 46071 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Garc A Luis, A" sort="Garc A Luis, A" uniqKey="Garc A Luis A" first="A" last="Garc A-Luis">A. Garc A-Luis</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologı́a Vegetal, Universidad Politécnica de Valencia, PO Box 22012, 46071 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Espagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Scientia Horticulturae</title><title level="j" type="abbrev">HORTI</title><idno type="ISSN">0304-4238</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">87</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="275">275</biblScope><biblScope unit="page" to="290">290</biblScope></imprint><idno type="ISSN">0304-4238</idno></series><idno type="istex">C2490CE6B4DA818482D63532ED8C4BFA2D0D403F</idno><idno type="DOI">10.1016/S0304-4238(00)00186-2</idno><idno type="PII">S0304-4238(00)00186-2</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-4238</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adventitious shoots</term><term>Citrus sinensis×Poncirus trifoliata</term><term>Daylength</term><term>Direct organogenesis</term><term>Growth regulators</term><term>Indirect organogenesis</term><term>Morphogenesis</term><term>Organogenesis</term><term>Photon flux density</term><term>Troyer citrange</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The optimal growth regulator addenda for adventitious shoot regeneration in epicotyl cuttings of Troyer citrange (Citrus sinensis×Poncirus trifoliata) varied with the conditions of illumination. The response to illumination and to growth regulators differed for the direct and the indirect (through callusing) pathways of regeneration. Shoot formation through the direct organogenic pathway decreased as the concentration of benzyladenine (BA) in the medium was increased in the range 2.2–22μM, when the explants were incubated in the dark or under an 8h daylength. For explants incubated under a 16h daylength, the number of shoots formed increased with BA concentration. Optimal conditions of incubation for shoot formation through the direct pathway were either an 8h daylength with a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA, or a 16h daylength with a photon flux density of 37μmolm−2s−1 in the presence of 22μM BA. Irrespective of the conditions of incubation, shoot formation through the indirect organogenic pathway was suppressed by the addition of 22μM BA to the medium. Optimal conditions for shoot formation through this pathway were incubation under an 8h daylength at a photon flux density of 74μmolm−2s−1 in the presence of 2.2μM BA. At the optimal conditions indicated, the addition of the synthetic auxin naphtaleneacetic acid (0.54μM) reduced shoot formation. Irrespective of the pathway of regeneration, the number of shoots formed decreased markedly with the distance of the cutting from the cotyledonary node.</div></front></TEI></ISTEX></double></record>Pour manipuler ce document sous Unix (Dilib)
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