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Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus: application to monitoring cross-protection

Identifieur interne : 002C76 ( Main/Curation ); précédent : 002C75; suivant : 002C77

Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus: application to monitoring cross-protection

Auteurs : A. Sambade [Espagne] ; L. Rubio [Espagne] ; S. M. Garnsey [États-Unis] ; N. Costa [Argentine] ; G. W. Müller [Brésil] ; M. Peyrou [Uruguay] ; J. Guerri [Espagne] ; P. Moreno [Espagne]

Source :

Plant pathology [ 0032-0862 ] ; 2002.

RBID : Pascal:03-0493545

Descripteurs français

Pascal (Inist)
- Diversité génétique, Origine géographique, Pouvoir pathogène, Structure population, Protection croisée, Surveillance population, Gène, Méthode analyse, Polymorphisme conformation simple brin, Isolat, Variant, Citrus sinensis, Virus tristeza agrume, Marqueur moléculaire, DNA complémentaire, RNA monocaténaire, Bassin méditerranéen, Zone subtropicale, Zone tropicale, Etude expérimentale, Etude méthode, Séquence nucléotide, Symptomatologie, Variabilité génétique, Variation géographique, Variation phénotypique, Indexation, Identification génétique, Biotype, Génotype, Pathotype, Argentine, Brésil, Corse, Espagne, Floride, Israël, Japon, Uruguay.
Wicri :
- geographic : Argentine, Brésil, Collectivité territoriale de Corse, Espagne, Israël, Japon, Uruguay.
- topic : Zone subtropicale, Zone tropicale.

English descriptors

KwdEn :
- Citrus sinensis, Citrus tristeza virus, Cross protection, Isolate, Mediterranean Basin, Pathogenicity, Population structure, Population survey, Single stranded RNA, Subtropical zone, Tropical zone.

Abstract

The population of sequence variants of Citrus tristeza virus (CTV) isolates of different geographic origins and pathogenicity properties was characterized by single-strand conformation polymorphism (SSCP) analysis of cDNA of the genes p18, p13, p20 and p23. The mild isolates analysed here usually yielded a SSCP profile with two DNA bands, suggestive of a predominant sequence variant, whereas the SSCP profile of the most virulent isolates contained more than two DNA bands, indicating that their viral populations are likely to be more complex. The set of SSCP profiles of the four genes allowed identification of individual isolates, but no profile characteristic of a geographic area or a biogroup was found.[...]

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Pascal:03-0493545

Le document en format XML

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<author><name sortKey="Sambade, A" sort="Sambade, A" uniqKey="Sambade A" first="A." last="Sambade">A. Sambade</name>
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<s2>46113 Moncada, Valencia</s2>
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<author><name sortKey="Peyrou, M" sort="Peyrou, M" uniqKey="Peyrou M" first="M." last="Peyrou">M. Peyrou</name>
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<author><name sortKey="Moreno, P" sort="Moreno, P" uniqKey="Moreno P" first="P." last="Moreno">P. Moreno</name>
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<author><name sortKey="Sambade, A" sort="Sambade, A" uniqKey="Sambade A" first="A." last="Sambade">A. Sambade</name>
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<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias, Cra. Moncada-Náquera Km. 45</s1>
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<author><name sortKey="Costa, N" sort="Costa, N" uniqKey="Costa N" first="N." last="Costa">N. Costa</name>
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<country>Argentine</country>
<wicri:noRegion>INTA EEA Concordia CC 34(3200)</wicri:noRegion>
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<author><name sortKey="Muller, G W" sort="Muller, G W" uniqKey="Muller G" first="G. W." last="Müller">G. W. Müller</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Centro de Citricultura Sylvio Moreira, IAC, Rodovia Anhanguera Km. 158</s1>
<s2>Cordeirópolis-13490-970, S.P.</s2>
<s3>BRA</s3>
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</inist:fA14>
<country>Brésil</country>
<wicri:noRegion>Cordeirópolis-13490-970, S.P.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Peyrou, M" sort="Peyrou, M" uniqKey="Peyrou M" first="M." last="Peyrou">M. Peyrou</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Instituto de Investigaciones Bioldgicas Clemente Estable, Avenida. Italia</s1>
<s2>3318, Montevideo</s2>
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<wicri:noRegion>3318, Montevideo</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guerri, J" sort="Guerri, J" uniqKey="Guerri J" first="J." last="Guerri">J. Guerri</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias, Cra. Moncada-Náquera Km. 45</s1>
<s2>46113 Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
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<sZ>7 aut.</sZ>
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<wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Moreno, P" sort="Moreno, P" uniqKey="Moreno P" first="P." last="Moreno">P. Moreno</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias, Cra. Moncada-Náquera Km. 45</s1>
<s2>46113 Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant pathology</title>
<title level="j" type="abbreviated">Plant pathol.</title>
<idno type="ISSN">0032-0862</idno>
<imprint><date when="2002">2002</date>
</imprint>
</series>
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<seriesStmt><title level="j" type="main">Plant pathology</title>
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<idno type="ISSN">0032-0862</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis</term>
<term>Citrus tristeza virus</term>
<term>Cross protection</term>
<term>Isolate</term>
<term>Mediterranean Basin</term>
<term>Pathogenicity</term>
<term>Population structure</term>
<term>Population survey</term>
<term>Single stranded RNA</term>
<term>Subtropical zone</term>
<term>Tropical zone</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Diversité génétique</term>
<term>Origine géographique</term>
<term>Pouvoir pathogène</term>
<term>Structure population</term>
<term>Protection croisée</term>
<term>Surveillance population</term>
<term>Gène</term>
<term>Méthode analyse</term>
<term>Polymorphisme conformation simple brin</term>
<term>Isolat</term>
<term>Variant</term>
<term>Citrus sinensis</term>
<term>Virus tristeza agrume</term>
<term>Marqueur moléculaire</term>
<term>DNA complémentaire</term>
<term>RNA monocaténaire</term>
<term>Bassin méditerranéen</term>
<term>Zone subtropicale</term>
<term>Zone tropicale</term>
<term>Etude expérimentale</term>
<term>Etude méthode</term>
<term>Séquence nucléotide</term>
<term>Symptomatologie</term>
<term>Variabilité génétique</term>
<term>Variation géographique</term>
<term>Variation phénotypique</term>
<term>Indexation</term>
<term>Identification génétique</term>
<term>Biotype</term>
<term>Génotype</term>
<term>Pathotype</term>
<term>Argentine</term>
<term>Brésil</term>
<term>Corse</term>
<term>Espagne</term>
<term>Floride</term>
<term>Israël</term>
<term>Japon</term>
<term>Uruguay</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Argentine</term>
<term>Brésil</term>
<term>Collectivité territoriale de Corse</term>
<term>Espagne</term>
<term>Israël</term>
<term>Japon</term>
<term>Uruguay</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Zone subtropicale</term>
<term>Zone tropicale</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The population of sequence variants of Citrus tristeza virus (CTV) isolates of different geographic origins and pathogenicity properties was characterized by single-strand conformation polymorphism (SSCP) analysis of cDNA of the genes p18, p13, p20 and p23. The mild isolates analysed here usually yielded a SSCP profile with two DNA bands, suggestive of a predominant sequence variant, whereas the SSCP profile of the most virulent isolates contained more than two DNA bands, indicating that their viral populations are likely to be more complex. The set of SSCP profiles of the four genes allowed identification of individual isolates, but no profile characteristic of a geographic area or a biogroup was found.[...]</div>
</front>
</TEI>
</record>

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Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus: application to monitoring cross-protection

Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus: application to monitoring cross-protection

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri