Development and validation of a highly sensitive LC‐MS/MS‐ESI method for the determination of nobiletin in rat plasma: application to a pharmacokinetic study
Identifieur interne : 001524 ( Main/Curation ); précédent : 001523; suivant : 001525Development and validation of a highly sensitive LC‐MS/MS‐ESI method for the determination of nobiletin in rat plasma: application to a pharmacokinetic study
Auteurs : Avinash Kumar [Inde] ; V. C. Devaraj [Inde] ; Kalpesh C. Giri [Inde] ; Sanjeev Giri [Inde] ; Sriram Rajagopal [Inde] ; Ramesh Mullangi [Inde]Source :
- Biomedical Chromatography [ 0269-3879 ] ; 2012-12.
Abstract
A highly sensitive, rapid assay method has been developed and validated for the estimation of nobiletin in rat plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive‐ion mode. The assay procedure involves extraction of nobiletin and citalopram (internal standard, IS) from rat plasma with liquid–liquid extraction. Chromatographic separation was achieved using an isocratic mobile phase (0.2% formic acid–acetonitrile, 20:80, v/v) at a flow rate of 0.6 mL/min on an Atlantis dC18 column (maintained at 40 ± 1 °C) with a total run time of 2.0 min. The MS/MS ion transitions monitored were 403.2 → 373.0 for nobiletin and 325.2 → 109.0 for IS. Method validation was performed as per Food and Drug Administration guidelines and the results met the acceptance criteria. The lower limit of quantitation achieved was 0.05 ng/mL and the linearity range extended from 0.05 to 51.98 ng/mL. The intra‐ and inter‐day precisions were in the range of 1.96–14.3 and 6.21–12.1, respectively. Copyright © 2012 John Wiley & Sons, Ltd.
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DOI: 10.1002/bmc.2717
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Chromatogr.</title><idno type="ISSN">0269-3879</idno><idno type="eISSN">1099-0801</idno><imprint><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2012-12">2012-12</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1464">1464</biblScope><biblScope unit="page" to="1471">1471</biblScope></imprint><idno type="ISSN">0269-3879</idno></series><idno type="istex">03B8AFC153F1B9E13FFFAC2DB9C9BF74C3AA8742</idno><idno type="DOI">10.1002/bmc.2717</idno><idno type="ArticleID">BMC2717</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0269-3879</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">A highly sensitive, rapid assay method has been developed and validated for the estimation of nobiletin in rat plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive‐ion mode. The assay procedure involves extraction of nobiletin and citalopram (internal standard, IS) from rat plasma with liquid–liquid extraction. Chromatographic separation was achieved using an isocratic mobile phase (0.2% formic acid–acetonitrile, 20:80, v/v) at a flow rate of 0.6 mL/min on an Atlantis dC18 column (maintained at 40 ± 1 °C) with a total run time of 2.0 min. The MS/MS ion transitions monitored were 403.2 → 373.0 for nobiletin and 325.2 → 109.0 for IS. Method validation was performed as per Food and Drug Administration guidelines and the results met the acceptance criteria. The lower limit of quantitation achieved was 0.05 ng/mL and the linearity range extended from 0.05 to 51.98 ng/mL. The intra‐ and inter‐day precisions were in the range of 1.96–14.3 and 6.21–12.1, respectively. Copyright © 2012 John Wiley & Sons, Ltd.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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