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Polymethoxylated flavones in Brazilian orange juice

Identifieur interne : 000838 ( Istex/Corpus ); précédent : 000837; suivant : 000839

Polymethoxylated flavones in Brazilian orange juice

Auteurs : A. M. Pupin ; M. J. Dennis ; M. C. F. Toledo

Source :

RBID : ISTEX:7A048ADF7C1C193CCCED50D5453020F95BE445C9

Abstract

Sinensetin has been quantified in authentic samples of Brazilian orange juice. In addition, six further polymethoxylated flavones (PMFs) have been determined in terms of their relative amounts. The PMFs were extracted into toluene and analysed using reversed phase HPLC with detection at 340nm. Peak identification was based on the UV-visible spectra and the elution order described in the literature. Hand-squeezed orange juices contained a mean of 0.10 (SD 0.04) mg−1 sinensetin with the highest concentrations found in Pera and Natal varieties. Commercial samples of frozen concentrated orange juice (FCOJ), frozen concentrated pulp-wash (FCOPW), retail FCOJ and retail freshly squeezed orange juice (FSOJ) typically contained at least ten times more sinensetin than those found for samples squeezed by hand. The PMFs peak area ratios for these sample classes were examined further using canonical discriminant analysis. This procedure could distinguish the hand-squeezed juices of Pera and Hamlin varieties from those of Natal and Valência. Similarly, hand-squeezed juices could be readily distinguished from the commercial samples of FCOJ, FCOPW, retail FCOJ and retail FSOJ.

Url:
DOI: 10.1016/S0308-8146(98)00033-8

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ISTEX:7A048ADF7C1C193CCCED50D5453020F95BE445C9

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<div type="abstract" xml:lang="en">Sinensetin has been quantified in authentic samples of Brazilian orange juice. In addition, six further polymethoxylated flavones (PMFs) have been determined in terms of their relative amounts. The PMFs were extracted into toluene and analysed using reversed phase HPLC with detection at 340nm. Peak identification was based on the UV-visible spectra and the elution order described in the literature. Hand-squeezed orange juices contained a mean of 0.10 (SD 0.04) mg−1 sinensetin with the highest concentrations found in Pera and Natal varieties. Commercial samples of frozen concentrated orange juice (FCOJ), frozen concentrated pulp-wash (FCOPW), retail FCOJ and retail freshly squeezed orange juice (FSOJ) typically contained at least ten times more sinensetin than those found for samples squeezed by hand. The PMFs peak area ratios for these sample classes were examined further using canonical discriminant analysis. This procedure could distinguish the hand-squeezed juices of Pera and Hamlin varieties from those of Natal and Valência. Similarly, hand-squeezed juices could be readily distinguished from the commercial samples of FCOJ, FCOPW, retail FCOJ and retail FSOJ.</div>
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<note type="content">Fig. 1: HPLC of PMFs from an orange juice extract. Chromatographic conditions: Column C18 Nucleosil 5μm (250×4.6mm i.d., Alltech), pre-column C18μm (7.5×4.6mm i.d., Alltech), mobile phase water:acetonitrile:tetrahydrofuran (53:43:3), flow 0.7mlmin−1, UV 340nm. Peak I sinensetin, II quercetogetin, III nobiletin, IV heptamethoxyflavone, V scutellarein, VI tangeretin.</note>
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<note type="content">Table 1: Relative peak areas of PMFs and level of sinensetin (mgl−1) for the IHRM</note>
<note type="content">Table 2: Relative peak area of PMFs and sinensetin levels (mgl−1) in authentic hand-squeezed samples of orange juice (see Table 1 for peak identification)</note>
<note type="content">Table 3: Relative peak area of PMFs and sinensetin levels (mgl−1) in authentic samples of frozen concentrated orange juice (FCOJ) and in authentic samples of frozen concentrated pulp-wash (FCOPW), both diluted to 12° Brix (see Table 1 for peak identification)</note>
<note type="content">Table 4: Relative peak area of PMFs and sinensetin levels (mgl−1) in retail samples of frozen concentrated orange juice (RFCOJ), diluted to 12° Brix (see Table 1 for peak identification)</note>
<note type="content">Table 5: Relative peak area of PMFs and sinensetin levels (mgl−1) in retail samples of freshly squeezed orange (see Table 1 for peak identification)</note>
<note type="content">Table 6: Summary for peak ratio in authentic (hand-squeezed) and in authentic samples of orange juice from literature (see Table 1 for peak identification)</note>
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<abstract lang="en">Sinensetin has been quantified in authentic samples of Brazilian orange juice. In addition, six further polymethoxylated flavones (PMFs) have been determined in terms of their relative amounts. The PMFs were extracted into toluene and analysed using reversed phase HPLC with detection at 340nm. Peak identification was based on the UV-visible spectra and the elution order described in the literature. Hand-squeezed orange juices contained a mean of 0.10 (SD 0.04) mg−1 sinensetin with the highest concentrations found in Pera and Natal varieties. Commercial samples of frozen concentrated orange juice (FCOJ), frozen concentrated pulp-wash (FCOPW), retail FCOJ and retail freshly squeezed orange juice (FSOJ) typically contained at least ten times more sinensetin than those found for samples squeezed by hand. The PMFs peak area ratios for these sample classes were examined further using canonical discriminant analysis. This procedure could distinguish the hand-squeezed juices of Pera and Hamlin varieties from those of Natal and Valência. Similarly, hand-squeezed juices could be readily distinguished from the commercial samples of FCOJ, FCOPW, retail FCOJ and retail FSOJ.</abstract>
<note type="content">Fig. 1: HPLC of PMFs from an orange juice extract. Chromatographic conditions: Column C18 Nucleosil 5μm (250×4.6mm i.d., Alltech), pre-column C18μm (7.5×4.6mm i.d., Alltech), mobile phase water:acetonitrile:tetrahydrofuran (53:43:3), flow 0.7mlmin−1, UV 340nm. Peak I sinensetin, II quercetogetin, III nobiletin, IV heptamethoxyflavone, V scutellarein, VI tangeretin.</note>
<note type="content">Fig. 2: Canonical discriminant analysis of several kinds of orange juice using PMF data (FCOJ: frozen concentrated orange juice; FCOPW: frozen concentrated orange pulp-wash; FSOJ: freshly squeezed orange juice).</note>
<note type="content">Table 1: Relative peak areas of PMFs and level of sinensetin (mgl−1) for the IHRM</note>
<note type="content">Table 2: Relative peak area of PMFs and sinensetin levels (mgl−1) in authentic hand-squeezed samples of orange juice (see Table 1 for peak identification)</note>
<note type="content">Table 3: Relative peak area of PMFs and sinensetin levels (mgl−1) in authentic samples of frozen concentrated orange juice (FCOJ) and in authentic samples of frozen concentrated pulp-wash (FCOPW), both diluted to 12° Brix (see Table 1 for peak identification)</note>
<note type="content">Table 4: Relative peak area of PMFs and sinensetin levels (mgl−1) in retail samples of frozen concentrated orange juice (RFCOJ), diluted to 12° Brix (see Table 1 for peak identification)</note>
<note type="content">Table 5: Relative peak area of PMFs and sinensetin levels (mgl−1) in retail samples of freshly squeezed orange (see Table 1 for peak identification)</note>
<note type="content">Table 6: Summary for peak ratio in authentic (hand-squeezed) and in authentic samples of orange juice from literature (see Table 1 for peak identification)</note>
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<identifier type="ISSN">0308-8146</identifier>
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