Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw
Identifieur interne : 000579 ( Istex/Corpus ); précédent : 000578; suivant : 000580Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw
Auteurs : D. Sihachakr ; R. Haicour ; -H. Chaput ; E. Barrientos ; G. Ducreux ; L. RossignolSource :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1989-01-01.
Abstract
Summary: Somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.
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DOI: 10.1007/BF00292307
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title><author><name sortKey="Sihachakr, D" sort="Sihachakr, D" uniqKey="Sihachakr D" first="D." last="Sihachakr">D. Sihachakr</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Haicour, R" sort="Haicour, R" uniqKey="Haicour R" first="R." last="Haicour">R. Haicour</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Chaput, H" sort="Chaput, H" uniqKey="Chaput " first="-H." last="Chaput">-H. Chaput</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Barrientos, E" sort="Barrientos, E" uniqKey="Barrientos E" first="E." last="Barrientos">E. Barrientos</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Ducreux, G" sort="Ducreux, G" uniqKey="Ducreux G" first="G." last="Ducreux">G. Ducreux</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Rossignol, L" sort="Rossignol, L" uniqKey="Rossignol L" first="L." last="Rossignol">L. Rossignol</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:77D563149CD32FAC3D615B81229FBDE0505A066B</idno><date when="1989" year="1989">1989</date><idno type="doi">10.1007/BF00292307</idno><idno type="url">https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000579</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title><author><name sortKey="Sihachakr, D" sort="Sihachakr, D" uniqKey="Sihachakr D" first="D." last="Sihachakr">D. Sihachakr</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Haicour, R" sort="Haicour, R" uniqKey="Haicour R" first="R." last="Haicour">R. Haicour</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Chaput, H" sort="Chaput, H" uniqKey="Chaput " first="-H." last="Chaput">-H. Chaput</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Barrientos, E" sort="Barrientos, E" uniqKey="Barrientos E" first="E." last="Barrientos">E. Barrientos</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Ducreux, G" sort="Ducreux, G" uniqKey="Ducreux G" first="G." last="Ducreux">G. Ducreux</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Rossignol, L" sort="Rossignol, L" uniqKey="Rossignol L" first="L." last="Rossignol">L. Rossignol</name><affiliation><mods:affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1989-01-01">1989-01-01</date><biblScope unit="volume">77</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="6">6</biblScope></imprint><idno type="ISSN">0040-5752</idno></series><idno type="istex">77D563149CD32FAC3D615B81229FBDE0505A066B</idno><idno type="DOI">10.1007/BF00292307</idno><idno type="ArticleID">BF00292307</idno><idno type="ArticleID">Art1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>D. Sihachakr</name><affiliations><json:string>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</json:string></affiliations></json:item><json:item><name>R. Haicour</name><affiliations><json:string>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</json:string></affiliations></json:item><json:item><name>M. -H. Chaput</name><affiliations><json:string>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</json:string></affiliations></json:item><json:item><name>E. Barrientos</name><affiliations><json:string>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</json:string></affiliations></json:item><json:item><name>G. 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Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.</abstract><qualityIndicators><score>4.685</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1021</abstractCharCount><pdfWordCount>3005</pdfWordCount><pdfCharCount>18707</pdfCharCount><pdfPageCount>6</pdfPageCount><abstractWordCount>140</abstractWordCount></qualityIndicators><title>Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title><genre><json:string>research-article</json:string></genre><host><volume>77</volume><pages><last>6</last><first>1</first></pages><issn><json:string>0040-5752</json:string></issn><issue>1</issue><subject><json:item><value>Biotechnology</value></json:item><json:item><value>Agriculture</value></json:item><json:item><value>Biochemistry, general</value></json:item><json:item><value>Plant Biochemistry</value></json:item><json:item><value>Plant Sciences</value></json:item><json:item><value>Plant Genetics & Genomics</value></json:item></subject><journalId><json:string>122</json:string></journalId><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1432-2242</json:string></eissn><title>Theoretical and Applied Genetics</title><publicationDate>1989</publicationDate><copyrightDate>1989</copyrightDate></host><publicationDate>1989</publicationDate><copyrightDate>1989</copyrightDate><doi><json:string>10.1007/BF00292307</json:string></doi><id>77D563149CD32FAC3D615B81229FBDE0505A066B</id><score>0.33699974</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><p>Springer-Verlag, 1989</p></availability><date>1988-06-17</date></publicationStmt><notesStmt><note>Originals</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title><author xml:id="author-1" corresp="yes"><persName><forename type="first">D.</forename><surname>Sihachakr</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">R.</forename><surname>Haicour</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">M.</forename><surname>Chaput</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">E.</forename><surname>Barrientos</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">G.</forename><surname>Ducreux</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">L.</forename><surname>Rossignol</surname></persName><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation></author></analytic><monogr><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="journal-ID">122</idno><idno type="pISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><idno type="issue-article-count">25</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1989-01-01"></date><biblScope unit="volume">77</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="6">6</biblScope></imprint></monogr><idno type="istex">77D563149CD32FAC3D615B81229FBDE0505A066B</idno><idno type="DOI">10.1007/BF00292307</idno><idno type="ArticleID">BF00292307</idno><idno type="ArticleID">Art1</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1988-06-17</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: Somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biotechnology</term></item><item><term>Agriculture</term></item><item><term>Biochemistry, general</term></item><item><term>Plant Biochemistry</term></item><item><term>Plant Sciences</term></item><item><term>Plant Genetics & Genomics</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1988-06-17">Created</change><change when="1989-01-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-09-22">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>122</JournalID><JournalPrintISSN>0040-5752</JournalPrintISSN><JournalElectronicISSN>1432-2242</JournalElectronicISSN><JournalTitle>Theoretical and Applied Genetics</JournalTitle><JournalSubTitle>International Journal of Plant Breeding Research</JournalSubTitle><JournalAbbreviatedTitle>Theoret. 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Genetics</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Biotechnology</JournalSubject><JournalSubject Type="Secondary">Agriculture</JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Plant Biochemistry</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject><JournalSubject Type="Secondary">Plant Genetics & Genomics</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>77</VolumeIDStart><VolumeIDEnd>77</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>25</IssueArticleCount><IssueHistory><CoverDate><Year>1989</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1989</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art1"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00292307</ArticleID><ArticleDOI>10.1007/BF00292307</ArticleDOI><ArticleSequenceNumber>1</ArticleSequenceNumber><ArticleTitle Language="En">Somatic hybrid plants produced by electrofusion between <Emphasis Type="Italic">Solanum melongena</Emphasis> L. and <Emphasis Type="Italic">Solanum torvum</Emphasis> Sw</ArticleTitle><ArticleCategory>Originals</ArticleCategory><ArticleFirstPage>1</ArticleFirstPage><ArticleLastPage>6</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>8</Month><Day>17</Day></RegistrationDate><Received><Year>1988</Year><Month>6</Month><Day>17</Day></Received><Accepted><Year>1988</Year><Month>7</Month><Day>18</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1989</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>122</JournalID><VolumeIDStart>77</VolumeIDStart><VolumeIDEnd>77</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>D.</GivenName><FamilyName>Sihachakr</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>R.</GivenName><FamilyName>Haicour</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><GivenName>-H.</GivenName><FamilyName>Chaput</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>E.</GivenName><FamilyName>Barrientos</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><FamilyName>Ducreux</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>L.</GivenName><FamilyName>Rossignol</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360</OrgDivision><OrgName>Université Paris Sud</OrgName><OrgAddress><Postcode>F-91405</Postcode><City>Orsay Cedex</City><Country>France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Somatic hybrid plants between eggplant (<Emphasis Type="Italic">Solanum melongena</Emphasis>) and <Emphasis Type="Italic">Solanum torvum</Emphasis> have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword><Emphasis Type="Italic">Solanum melongena</Emphasis></Keyword><Keyword><Emphasis Type="Italic">Solanum torvum</Emphasis></Keyword><Keyword>Protoplasts</Keyword><Keyword>Electrofusion</Keyword><Keyword>Somatic hybrids</Keyword></KeywordGroup><ArticleNote Type="CommunicatedBy"><SimplePara>Communicated by I. Potrykus</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">D.</namePart><namePart type="family">Sihachakr</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Haicour</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="given">-H.</namePart><namePart type="family">Chaput</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Barrientos</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Ducreux</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Rossignol</namePart><affiliation>Laboratoire de Morphogénèse Végétale Expérimentale, CNRS UA 115, Bât 360, Université Paris Sud, F-91405, Orsay Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">1988-06-17</dateCreated><dateIssued encoding="w3cdtf">1989-01-01</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Summary: Somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum have been produced by the electrofusion of mesophyll protoplasts in a movable multi-electrode fusion chamber. Using hair structure as a selection criteria, we identified a total of 19 somatic hybrids, which represented an overall average of 15.3% of the 124 regenerated plants obtained in the two fusion experiments. Several morphological traits were intermediate to those of the parents, including trichome density and structure, height, leaf form and inflorescence. Cytological analyses revealed that the chromosome numbers of the somatic hybrids approximated the expected tetraploid level (2n=4x=48). Fifteen hybrid plants were homogeneous and had relatively stable chromosome numbers (46–48), while four other hybrids had variable chromosome numbers (35–48) and exhibited greater morphological variation. The hybridity of these 19 somatic hybrid plants was confirmed by analyses of phosphoglucomutase (Pgm) and esterase zymograms.</abstract><note>Originals</note><relatedItem type="host"><titleInfo><title>Theoretical and Applied Genetics</title><subTitle>International Journal of Plant Breeding Research</subTitle></titleInfo><titleInfo type="abbreviated"><title>Theoret. Appl. Genetics</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1989-01-01</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biotechnology</topic><topic>Agriculture</topic><topic>Biochemistry, general</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Plant Genetics & Genomics</topic></subject><identifier type="ISSN">0040-5752</identifier><identifier type="eISSN">1432-2242</identifier><identifier type="JournalID">122</identifier><identifier type="IssueArticleCount">25</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1989</date><detail type="volume"><number>77</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>1</start><end>6</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1989</recordOrigin></recordInfo></relatedItem><identifier type="istex">77D563149CD32FAC3D615B81229FBDE0505A066B</identifier><identifier type="DOI">10.1007/BF00292307</identifier><identifier type="ArticleID">BF00292307</identifier><identifier type="ArticleID">Art1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1989</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Springer-Verlag, 1989</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/enrichments/multicat</uri></json:item><json:item><type>refBibs</type><uri>https://api.istex.fr/document/77D563149CD32FAC3D615B81229FBDE0505A066B/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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