Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity
Identifieur interne : 000503 ( Istex/Corpus ); précédent : 000502; suivant : 000504Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity
Auteurs : Miguel Cerezo ; Pilar Garc A-Agust N ; M Dolores Serna ; Eduardo Primo-MilloSource :
- Plant Science [ 0168-9452 ] ; 1997.
Abstract
Nitrate absorption by roots of both Troyer citrange (hybrid of Citrus sinensis×Poncirus trifoliata) and Cleopatra mandarin (C. reshni) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO3− net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO3− concentrations up to 10 mol m−3. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m−3 NO3− in both rootstocks. The apparent Kms of nitrate uptake were 282 and 281 mmol m−3 respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO3− concentrations (between 1–10 mol m−3). Double reciprocal plots showed that Cl− inhibited NO3− uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl−, but was inhibited when plants were pretreated with salt solutions. Net NO3− uptake in both systems was severely affected by Cl− ions more in Troyer citrange than in Cleopatra mandarin. Since Cl− accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl− and the inhibition of NO3− uptake is suggested for these plants.
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DOI: 10.1016/S0168-9452(97)00095-2
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title><author><name sortKey="Cerezo, Miguel" sort="Cerezo, Miguel" uniqKey="Cerezo M" first="Miguel" last="Cerezo">Miguel Cerezo</name><affiliation><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Garc A Agust N, Pilar" sort="Garc A Agust N, Pilar" uniqKey="Garc A Agust N P" first="Pilar" last="Garc A-Agust N">Pilar Garc A-Agust N</name><affiliation><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Serna, M Dolores" sort="Serna, M Dolores" uniqKey="Serna M" first="M Dolores" last="Serna">M Dolores Serna</name><affiliation><mods:affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</mods:affiliation></affiliation></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name><affiliation><mods:affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:53BC3AF98D5D9A59C753762C709F3B7820C6F066</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1016/S0168-9452(97)00095-2</idno><idno type="url">https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000503</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title><author><name sortKey="Cerezo, Miguel" sort="Cerezo, Miguel" uniqKey="Cerezo M" first="Miguel" last="Cerezo">Miguel Cerezo</name><affiliation><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Garc A Agust N, Pilar" sort="Garc A Agust N, Pilar" uniqKey="Garc A Agust N P" first="Pilar" last="Garc A-Agust N">Pilar Garc A-Agust N</name><affiliation><mods:affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Serna, M Dolores" sort="Serna, M Dolores" uniqKey="Serna M" first="M Dolores" last="Serna">M Dolores Serna</name><affiliation><mods:affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</mods:affiliation></affiliation></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name><affiliation><mods:affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="ISSN">0168-9452</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">126</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="105">105</biblScope><biblScope unit="page" to="112">112</biblScope></imprint><idno type="ISSN">0168-9452</idno></series><idno type="istex">53BC3AF98D5D9A59C753762C709F3B7820C6F066</idno><idno type="DOI">10.1016/S0168-9452(97)00095-2</idno><idno type="PII">S0168-9452(97)00095-2</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-9452</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nitrate absorption by roots of both Troyer citrange (hybrid of Citrus sinensis×Poncirus trifoliata) and Cleopatra mandarin (C. reshni) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO3− net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO3− concentrations up to 10 mol m−3. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m−3 NO3− in both rootstocks. The apparent Kms of nitrate uptake were 282 and 281 mmol m−3 respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO3− concentrations (between 1–10 mol m−3). Double reciprocal plots showed that Cl− inhibited NO3− uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl−, but was inhibited when plants were pretreated with salt solutions. Net NO3− uptake in both systems was severely affected by Cl− ions more in Troyer citrange than in Cleopatra mandarin. Since Cl− accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl− and the inhibition of NO3− uptake is suggested for these plants.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Miguel Cerezo</name><affiliations><json:string>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</json:string></affiliations></json:item><json:item><name>Pilar Garcı́a-Agustı́n</name><affiliations><json:string>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</json:string></affiliations></json:item><json:item><name>Mª Dolores Serna</name><affiliations><json:string>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</json:string></affiliations></json:item><json:item><name>Eduardo Primo-Millo</name><affiliations><json:string>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Citrus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chloride</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Nitrate uptake</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Inhibition</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Salinity</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Nitrate absorption by roots of both Troyer citrange (hybrid of Citrus sinensis×Poncirus trifoliata) and Cleopatra mandarin (C. reshni) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO3− net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO3− concentrations up to 10 mol m−3. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m−3 NO3− in both rootstocks. The apparent Kms of nitrate uptake were 282 and 281 mmol m−3 respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO3− concentrations (between 1–10 mol m−3). Double reciprocal plots showed that Cl− inhibited NO3− uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl−, but was inhibited when plants were pretreated with salt solutions. Net NO3− uptake in both systems was severely affected by Cl− ions more in Troyer citrange than in Cleopatra mandarin. Since Cl− accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl− and the inhibition of NO3− uptake is suggested for these plants.</abstract><qualityIndicators><score>5.977</score><pdfVersion>1.1</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1320</abstractCharCount><pdfWordCount>3565</pdfWordCount><pdfCharCount>20441</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>201</abstractWordCount></qualityIndicators><title>Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title><pii><json:string>S0168-9452(97)00095-2</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>126</volume><pii><json:string>S0168-9452(00)X0039-8</json:string></pii><pages><last>112</last><first>105</first></pages><issn><json:string>0168-9452</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Plant Science</title><publicationDate>1997</publicationDate></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string><json:string>PLANT SCIENCES</json:string></wos></categories><publicationDate>1997</publicationDate><copyrightDate>1997</copyrightDate><doi><json:string>10.1016/S0168-9452(97)00095-2</json:string></doi><id>53BC3AF98D5D9A59C753762C709F3B7820C6F066</id><score>0.3610602</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1997 Elsevier Science Ireland Ltd</p></availability><date>1997</date></publicationStmt><notesStmt><note type="content">Fig. 1: Nitrate uptake rate as a function of substrate NO3− concentration by Cleopatra mandarin (a) and Troyer citrange (b) seedlings.</note><note type="content">Fig. 2: Effect of NaCl on the kinetics of NO3− uptake by the HATS in seedlings of Cleopatra mandarin (a) and Troyer citrange (b).</note><note type="content">Fig. 3: Effect of pretreatments with NaCl (30, 60 and 120 mol m−3) for 21 days on net NO3− uptake rate by roots of Troyer citrange and Cleopatra mandarin at 3 (a) and 6 (b) mol m−3 of external NO3−.</note><note type="content">Fig. 4: Relationship between net NO3− uptake rates and Cl− concentration in roots of Troyer citrange and Cleopatra mandarin after pretreatments with increasing NaCl solutions (0, 30, 60 and 120 mol m−3) for 21 days. (a) external [NO3−]=3 mol m−3; •, values for Troyer citrange; ○, values for Cleopatra mandarin; y=−0.0029x+0.6403, r2=0.95; (b) external [NO3−]=6 mol m−3; ■, values for Troyer citrange; □, values for Cleopatra mandarin; y=−0.0067x+1.4482, r2=0.93.</note><note type="content">Table 1: Kinetics constantsa of NO3− uptake by the HATS and inhibition by NaCl and KCl</note><note type="content">Table 2: Effect of NaCl on net NO3− uptake by the LATS</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title><author xml:id="author-1"><persName><forename type="first">Miguel</forename><surname>Cerezo</surname></persName><affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">Pilar</forename><surname>Garcı́a-Agustı́n</surname></persName><note type="correspondence"><p>Corresponding author.</p></note><affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">Mª Dolores</forename><surname>Serna</surname></persName><affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">Eduardo</forename><surname>Primo-Millo</surname></persName><affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</affiliation></author></analytic><monogr><title level="j">Plant Science</title><title level="j" type="abbrev">PSL</title><idno type="pISSN">0168-9452</idno><idno type="PII">S0168-9452(00)X0039-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997"></date><biblScope unit="volume">126</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="105">105</biblScope><biblScope unit="page" to="112">112</biblScope></imprint></monogr><idno type="istex">53BC3AF98D5D9A59C753762C709F3B7820C6F066</idno><idno type="DOI">10.1016/S0168-9452(97)00095-2</idno><idno type="PII">S0168-9452(97)00095-2</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Nitrate absorption by roots of both Troyer citrange (hybrid of Citrus sinensis×Poncirus trifoliata) and Cleopatra mandarin (C. reshni) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO3− net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO3− concentrations up to 10 mol m−3. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m−3 NO3− in both rootstocks. The apparent Kms of nitrate uptake were 282 and 281 mmol m−3 respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO3− concentrations (between 1–10 mol m−3). Double reciprocal plots showed that Cl− inhibited NO3− uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl−, but was inhibited when plants were pretreated with salt solutions. Net NO3− uptake in both systems was severely affected by Cl− ions more in Troyer citrange than in Cleopatra mandarin. Since Cl− accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl− and the inhibition of NO3− uptake is suggested for these plants.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Citrus</term></item><item><term>Chloride</term></item><item><term>Nitrate uptake</term></item><item><term>Inhibition</term></item><item><term>Salinity</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1997-04-02">Modified</change><change when="1997">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>PSL</jid><aid>4664</aid><ce:pii>S0168-9452(97)00095-2</ce:pii><ce:doi>10.1016/S0168-9452(97)00095-2</ce:doi><ce:copyright year="1997" type="full-transfer">Elsevier Science Ireland Ltd</ce:copyright></item-info><head><ce:title>Kinetics of nitrate uptake by <ce:italic>Citrus</ce:italic> seedlings and inhibitory effects of salinity</ce:title><ce:author-group><ce:author><ce:given-name>Miguel</ce:given-name><ce:surname>Cerezo</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Pilar</ce:given-name><ce:surname>Garcı́a-Agustı́n</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Mª Dolores</ce:given-name><ce:surname>Serna</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>Eduardo</ce:given-name><ce:surname>Primo-Millo</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="12" month="12" year="1996"></ce:date-received><ce:date-revised day="2" month="4" year="1997"></ce:date-revised><ce:date-accepted day="9" month="4" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Nitrate absorption by roots of both Troyer citrange (hybrid of <ce:italic>Citrus sinensis×Poncirus</ce:italic> <ce:italic>trifoliata</ce:italic>) and Cleopatra mandarin (<ce:italic>C.</ce:italic> <ce:italic>reshni</ce:italic>) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> concentrations up to 10 mol m<ce:sup>−3</ce:sup>. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m<ce:sup>−3</ce:sup> NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> in both rootstocks. The apparent <ce:italic>K</ce:italic><ce:inf>m</ce:inf>s of nitrate uptake were 282 and 281 mmol m<ce:sup>−3</ce:sup> respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> concentrations (between 1–10 mol m<ce:sup>−3</ce:sup>). Double reciprocal plots showed that Cl<ce:sup>−</ce:sup> inhibited NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl<ce:sup>−</ce:sup>, but was inhibited when plants were pretreated with salt solutions. Net NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> uptake in both systems was severely affected by Cl<ce:sup>−</ce:sup> ions more in Troyer citrange than in Cleopatra mandarin. Since Cl<ce:sup>−</ce:sup> accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl<ce:sup>−</ce:sup> and the inhibition of NO<ce:inf>3</ce:inf><ce:sup>−</ce:sup> uptake is suggested for these plants.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Citrus</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Chloride</ce:text></ce:keyword><ce:keyword><ce:text>Nitrate uptake</ce:text></ce:keyword><ce:keyword><ce:text>Inhibition</ce:text></ce:keyword><ce:keyword><ce:text>Salinity</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Kinetics of nitrate uptake by Citrus seedlings and inhibitory effects of salinity</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Kinetics of nitrate uptake by</title></titleInfo><name type="personal"><namePart type="given">Miguel</namePart><namePart type="family">Cerezo</namePart><affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pilar</namePart><namePart type="family">Garcı́a-Agustı́n</namePart><affiliation>Departamento de Ciencias Experimentales, Unidad de Biotecnologı́a Vegetal, Universidad Jaume I, Campus Borriol, Apartado 224, 12 080 Castellón, Spain</affiliation><description>Corresponding author.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mª Dolores</namePart><namePart type="family">Serna</namePart><affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eduardo</namePart><namePart type="family">Primo-Millo</namePart><affiliation>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial 4113, Moncada (Valencia), Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued><dateModified encoding="w3cdtf">1997-04-02</dateModified><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Nitrate absorption by roots of both Troyer citrange (hybrid of Citrus sinensis×Poncirus trifoliata) and Cleopatra mandarin (C. reshni) seedlings was studied. The ion depletion in the external nutrient solutions was used to estimate NO3− net uptake. In both rootstocks the kinetics of nitrate uptake showed a biphasic pattern in response to increasing external NO3− concentrations up to 10 mol m−3. The low concentration transport system followed Michaelis-Menten kinetics reaching saturation at 0.5 mol m−3 NO3− in both rootstocks. The apparent Kms of nitrate uptake were 282 and 281 mmol m−3 respectively, for Troyer citrange and Cleopatra mandarin. A linear system was also found at higher external NO3− concentrations (between 1–10 mol m−3). Double reciprocal plots showed that Cl− inhibited NO3− uptake competitively in the low concentration system. In contrast, the high concentration system was insensitive to external Cl−, but was inhibited when plants were pretreated with salt solutions. Net NO3− uptake in both systems was severely affected by Cl− ions more in Troyer citrange than in Cleopatra mandarin. Since Cl− accumulation in tissues was higher in Troyer citrange than in Cleopatra mandarin, a relationship between the ability to absorb Cl− and the inhibition of NO3− uptake is suggested for these plants.</abstract><note type="content">Fig. 1: Nitrate uptake rate as a function of substrate NO3− concentration by Cleopatra mandarin (a) and Troyer citrange (b) seedlings.</note><note type="content">Fig. 2: Effect of NaCl on the kinetics of NO3− uptake by the HATS in seedlings of Cleopatra mandarin (a) and Troyer citrange (b).</note><note type="content">Fig. 3: Effect of pretreatments with NaCl (30, 60 and 120 mol m−3) for 21 days on net NO3− uptake rate by roots of Troyer citrange and Cleopatra mandarin at 3 (a) and 6 (b) mol m−3 of external NO3−.</note><note type="content">Fig. 4: Relationship between net NO3− uptake rates and Cl− concentration in roots of Troyer citrange and Cleopatra mandarin after pretreatments with increasing NaCl solutions (0, 30, 60 and 120 mol m−3) for 21 days. (a) external [NO3−]=3 mol m−3; •, values for Troyer citrange; ○, values for Cleopatra mandarin; y=−0.0029x+0.6403, r2=0.95; (b) external [NO3−]=6 mol m−3; ■, values for Troyer citrange; □, values for Cleopatra mandarin; y=−0.0067x+1.4482, r2=0.93.</note><note type="content">Table 1: Kinetics constantsa of NO3− uptake by the HATS and inhibition by NaCl and KCl</note><note type="content">Table 2: Effect of NaCl on net NO3− uptake by the LATS</note><subject><genre>Keywords</genre><topic>Citrus</topic><topic>Chloride</topic><topic>Nitrate uptake</topic><topic>Inhibition</topic><topic>Salinity</topic></subject><relatedItem type="host"><titleInfo><title>Plant Science</title></titleInfo><titleInfo type="abbreviated"><title>PSL</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19970715</dateIssued></originInfo><identifier type="ISSN">0168-9452</identifier><identifier type="PII">S0168-9452(00)X0039-8</identifier><part><date>19970715</date><detail type="volume"><number>126</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>118</end></extent><extent unit="pages"><start>105</start><end>112</end></extent></part></relatedItem><identifier type="istex">53BC3AF98D5D9A59C753762C709F3B7820C6F066</identifier><identifier type="DOI">10.1016/S0168-9452(97)00095-2</identifier><identifier type="PII">S0168-9452(97)00095-2</identifier><accessCondition type="use and reproduction" contentType="copyright">©1997 Elsevier Science Ireland Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ireland Ltd, ©1997</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/53BC3AF98D5D9A59C753762C709F3B7820C6F066/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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