Characteristics of Citrus cell cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose
Identifieur interne : 000314 ( Istex/Corpus ); précédent : 000313; suivant : 000315Characteristics of Citrus cell cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose
Auteurs : Cécile Cabasson ; Patrick Ollitrault ; Francois-Xavier Côte ; Nicole Michaux-Ferriére ; Dominique Dambier ; Régine Dalnic ; Claude TeissonSource :
- Physiologia Plantarum [ 0031-9317 ] ; 1995-04.
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Abstract
Growth of willow‐leaf mandarin (Citrus deliciosa Ten.) nucellar callus cultures without embryo formation was supported on sucrose as the sole carbon source. Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. Finally, the phosphorus utilization rate per g dry weight formed was 2‐fold higher in cell cultures on galactose during the second half of the culture cycle, coinciding with further embryo ontogenesis.
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DOI: 10.1111/j.1399-3054.1995.tb06844.x
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ISTEX:E7B20A432D05345D94F072AADDBB4E4ED894BE77Le document en format XML
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Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. Finally, the phosphorus utilization rate per g dry weight formed was 2‐fold higher in cell cultures on galactose during the second half of the culture cycle, coinciding with further embryo ontogenesis.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Cécile Cabasson</name><affiliations><json:string>CIRAD‐BIOTROP, B. P. 5035. F‐34032 Montpellier, France</json:string></affiliations></json:item><json:item><name>Patrick Ollitrault</name><affiliations><json:string>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</json:string></affiliations></json:item><json:item><name>Francois‐Xavier Côte</name><affiliations><json:string>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</json:string></affiliations></json:item><json:item><name>Nicole Michaux‐Ferriére</name><affiliations><json:string>CIRAD‐BIOTROP, B. P. 5035. 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Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. 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Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. 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A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. 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F‐34032 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Patrick</namePart><namePart type="family">Ollitrault</namePart><affiliation>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</affiliation><description>Correspondence: P. Ollitrault (corresponding author)</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francois‐Xavier</namePart><namePart type="family">Côte</namePart><affiliation>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nicole</namePart><namePart type="family">Michaux‐Ferriére</namePart><affiliation>CIRAD‐BIOTROP, B. P. 5035. F‐34032 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dominique</namePart><namePart type="family">Dambier</namePart><affiliation>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Régine</namePart><namePart type="family">Dalnic</namePart><affiliation>CIRAD‐FLHOR, B. P. 5035, F‐34032 Montpellier, France.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Claude</namePart><namePart type="family">Teisson</namePart><affiliation>CIRAD‐BIOTROP, B. P. 5035. F‐34032 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1995-04</dateIssued><edition>Received 19 September, 1994 revised 28 October, 1994</edition><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">36</extent></physicalDescription><abstract lang="en">Growth of willow‐leaf mandarin (Citrus deliciosa Ten.) nucellar callus cultures without embryo formation was supported on sucrose as the sole carbon source. Somatic embryogenesis was obtained by substituting galactose for sucrose at the same concentration (0.15 M). A histocytological study revealed proliferation of young globular embryos during the first half of the 18‐day culture cycle, irrespective of the carbon source. However, during the second half of the cycle, further embryo development failed in cell cultures on sucrose‐containing medium. The rate of cell growth and the carbohydrate depletion of the medium were 3‐fold higher on sucrose than on galactose‐containing medium. Cell suspensions on sucrose differed by an increase in calcium, phosphorus, magnesium and potassium utilization soon after subculturing, followed by a sharp decrease. Finally, the phosphorus utilization rate per g dry weight formed was 2‐fold higher in cell cultures on galactose during the second half of the culture cycle, coinciding with further embryo ontogenesis.</abstract><subject lang="en"><genre>keywords</genre><topic>Cell suspension</topic><topic>Citrus deliciosa</topic><topic>galactose</topic><topic>histology</topic><topic>nutrition</topic><topic>somatic embryogenesis</topic><topic>sucrose</topic><topic>willow‐leaf mandarin</topic></subject><relatedItem type="host"><titleInfo><title>Physiologia Plantarum</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0031-9317</identifier><identifier type="eISSN">1399-3054</identifier><identifier type="DOI">10.1111/(ISSN)1399-3054</identifier><identifier type="PublisherID">PPL</identifier><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>93</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>464</start><end>470</end><total>7</total></extent></part></relatedItem><identifier type="istex">E7B20A432D05345D94F072AADDBB4E4ED894BE77</identifier><identifier type="DOI">10.1111/j.1399-3054.1995.tb06844.x</identifier><identifier type="ArticleID">PPL464</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/E7B20A432D05345D94F072AADDBB4E4ED894BE77/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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