Protoplast culture and plant regeneration in Lycium barbarum L.
Identifieur interne : 000295 ( Istex/Corpus ); précédent : 000294; suivant : 000296Protoplast culture and plant regeneration in Lycium barbarum L.
Auteurs : I. Ratushnyak ; M. Piven ; A. RudasSource :
- Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1989-01-01.
Abstract
Abstract: Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104–105 cells ml-1. After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process ‘protoplast — plant’. The results obtained allow us to conclude that L. barbarum can be used in the experiments on somatic hybridization or direct gene transfer.
Url:
DOI: 10.1007/BF00046866
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After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process ‘protoplast — plant’. The results obtained allow us to conclude that L. barbarum can be used in the experiments on somatic hybridization or direct gene transfer.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Plant Sciences</term></item><item><term>Plant Physiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1988-06-27">Created</change><change when="1989-01-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-09-22">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/03723C407C8902FB08608015F1E9FE90A5E49B81/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11240</JournalID><JournalPrintISSN>0167-6857</JournalPrintISSN><JournalElectronicISSN>1573-5044</JournalElectronicISSN><JournalTitle>Plant Cell, Tissue and Organ Culture</JournalTitle><JournalSubTitle>An International Journal on Biotechnology of Higher Plants</JournalSubTitle><JournalAbbreviatedTitle>Plant Cell Tiss Organ Cult</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject><JournalSubject Type="Secondary">Plant Physiology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>17</VolumeIDStart><VolumeIDEnd>17</VolumeIDEnd><VolumeIssueCount>3</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>2</IssueIDStart><IssueIDEnd>3</IssueIDEnd><IssueArticleCount>21</IssueArticleCount><IssueHistory><CoverDate><DateString>1989</DateString><Year>1989</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1989</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art14"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00046866</ArticleID><ArticleDOI>10.1007/BF00046866</ArticleDOI><ArticleSequenceNumber>14</ArticleSequenceNumber><ArticleTitle Language="En">Protoplast culture and plant regeneration in <Emphasis Type="Italic">Lycium barbarum</Emphasis> L.</ArticleTitle><ArticleCategory>General Papers</ArticleCategory><ArticleFirstPage>183</ArticleFirstPage><ArticleLastPage>190</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>5</Month><Day>1</Day></RegistrationDate><Received><Year>1988</Year><Month>6</Month><Day>27</Day></Received><Accepted><Year>1988</Year><Month>12</Month><Day>14</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1989</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>11240</JournalID><VolumeIDStart>17</VolumeIDStart><VolumeIDEnd>17</VolumeIDEnd><IssueIDStart>2</IssueIDStart><IssueIDEnd>3</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yakov</GivenName><GivenName>I.</GivenName><FamilyName>Ratushnyak</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Nickolai</GivenName><GivenName>M.</GivenName><FamilyName>Piven</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Vladimir</GivenName><GivenName>A.</GivenName><FamilyName>Rudas</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>N.G. Kholodny Institute of Botany</OrgDivision><OrgName>Ukrainian SSR Academy of Sciences</OrgName><OrgAddress><Street>Repina, 2</Street><Postcode>252601</Postcode><City>Kiev-GSP-1</City><Country>USSR</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Protoplasts were isolated from leaves of the woody plant <Emphasis Type="Italic">Lycium barbarum L.</Emphasis> and cultured in liquid nutrient medium TM-2 at a density of 10<Superscript>4</Superscript>–10<Superscript>5</Superscript> cells ml<Superscript>-1</Superscript>. After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process ‘protoplast — plant’. The results obtained allow us to conclude that <Emphasis Type="Italic">L. barbarum</Emphasis> can be used in the experiments on somatic hybridization or direct gene transfer.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword><Emphasis Type="Italic">Lycium barbarum</Emphasis></Keyword><Keyword>protoplast culture</Keyword><Keyword>callus</Keyword><Keyword>organogenesis</Keyword><Keyword>chromosome number, woody plants</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Protoplast culture and plant regeneration in Lycium barbarum L.</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Protoplast culture and plant regeneration in Lycium barbarum L.</title></titleInfo><name type="personal"><namePart type="given">Yakov</namePart><namePart type="given">I.</namePart><namePart type="family">Ratushnyak</namePart><affiliation>N.G. 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After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process ‘protoplast — plant’. 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