Prenyltransferases from the flavedo of Citrus sinensis
Identifieur interne : 000253 ( Istex/Corpus ); précédent : 000252; suivant : 000254Prenyltransferases from the flavedo of Citrus sinensis
Auteurs : Milton De La Fuente ; Luz M. Pérez ; Uta Hashagen ; Liliana Chayet ; Cecilia Rojas ; Gloria Portilla ; Osvaldo CoriSource :
- Phytochemistry [ 0031-9422 ] ; 1981.
Abstract
A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.
Url:
DOI: 10.1016/S0031-9422(00)98529-7
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Prenyltransferases from the flavedo of Citrus sinensis</title><author><name sortKey="De La Fuente, Milton" sort="De La Fuente, Milton" uniqKey="De La Fuente M" first="Milton" last="De La Fuente">Milton De La Fuente</name><affiliation><mods:affiliation>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</mods:affiliation></affiliation></author><author><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. Pérez</name></author><author><name sortKey="Hashagen, Uta" sort="Hashagen, Uta" uniqKey="Hashagen U" first="Uta" last="Hashagen">Uta Hashagen</name></author><author><name sortKey="Chayet, Liliana" sort="Chayet, Liliana" uniqKey="Chayet L" first="Liliana" last="Chayet">Liliana Chayet</name></author><author><name sortKey="Rojas, Cecilia" sort="Rojas, Cecilia" uniqKey="Rojas C" first="Cecilia" last="Rojas">Cecilia Rojas</name></author><author><name sortKey="Portilla, Gloria" sort="Portilla, Gloria" uniqKey="Portilla G" first="Gloria" last="Portilla">Gloria Portilla</name><affiliation><mods:affiliation>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</mods:affiliation></affiliation></author><author><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/S0031-9422(00)98529-7</idno><idno type="url">https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000253</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Prenyltransferases from the flavedo of Citrus sinensis</title><author><name sortKey="De La Fuente, Milton" sort="De La Fuente, Milton" uniqKey="De La Fuente M" first="Milton" last="De La Fuente">Milton De La Fuente</name><affiliation><mods:affiliation>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</mods:affiliation></affiliation></author><author><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. Pérez</name></author><author><name sortKey="Hashagen, Uta" sort="Hashagen, Uta" uniqKey="Hashagen U" first="Uta" last="Hashagen">Uta Hashagen</name></author><author><name sortKey="Chayet, Liliana" sort="Chayet, Liliana" uniqKey="Chayet L" first="Liliana" last="Chayet">Liliana Chayet</name></author><author><name sortKey="Rojas, Cecilia" sort="Rojas, Cecilia" uniqKey="Rojas C" first="Cecilia" last="Rojas">Cecilia Rojas</name></author><author><name sortKey="Portilla, Gloria" sort="Portilla, Gloria" uniqKey="Portilla G" first="Gloria" last="Portilla">Gloria Portilla</name><affiliation><mods:affiliation>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</mods:affiliation></affiliation></author><author><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name></author></analytic><monogr></monogr><series><title level="j">Phytochemistry</title><title level="j" type="abbrev">PHYTO</title><idno type="ISSN">0031-9422</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1551">1551</biblScope><biblScope unit="page" to="1557">1557</biblScope></imprint><idno type="ISSN">0031-9422</idno></series><idno type="istex">FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</idno><idno type="DOI">10.1016/S0031-9422(00)98529-7</idno><idno type="PII">S0031-9422(00)98529-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-9422</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Milton De la Fuente</name><affiliations><json:string>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</json:string></affiliations></json:item><json:item><name>Luz M. Pérez</name></json:item><json:item><name>Uta Hashagen</name></json:item><json:item><name>Liliana Chayet</name></json:item><json:item><name>Cecilia Rojas</name></json:item><json:item><name>Gloria Portilla</name><affiliations><json:string>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</json:string></affiliations></json:item><json:item><name>Osvaldo Cori</name></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Citrus sinensis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Rutaceae</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>prenyltransferases</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>neryl pyrophosphate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>geranyl pyrophosphate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>farnesol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>stereospecificity.</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IPP, isopentenyl pyrophosphate (C5)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DMAPP, 3,3′-dimethyl pyrophospate (C5)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GPP, geranyl pyrophospate (C10E)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>NPP, neryl pyrophospate (C10Z)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>(2E,6E)-FPP, (2E,6E)-farnesyl pyrophospate (C15)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>(2Z,6E)-FPP, (2Z,6E)-farnesyl pyrophospate (C15)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DTT, dithiothreithol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCMB</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>para-chloromercuribenzoate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DTNB, 5,5′-dithiobis(nitrobenzoic acid):</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.</abstract><qualityIndicators><score>5.221</score><pdfVersion>1.2</pdfVersion><pdfPageSize>505 x 721 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>17</keywordCount><abstractCharCount>930</abstractCharCount><pdfWordCount>3529</pdfWordCount><pdfCharCount>24702</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>141</abstractWordCount></qualityIndicators><title>Prenyltransferases from the flavedo of Citrus sinensis</title><pii><json:string>S0031-9422(00)98529-7</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>20</volume><pii><json:string>S0031-9422(00)X0405-0</json:string></pii><pages><last>1557</last><first>1551</first></pages><issn><json:string>0031-9422</json:string></issn><issue>7</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Phytochemistry</title><publicationDate>1981</publicationDate></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string><json:string>PLANT SCIENCES</json:string></wos></categories><publicationDate>1981</publicationDate><copyrightDate>1981</copyrightDate><doi><json:string>10.1016/S0031-9422(00)98529-7</json:string></doi><id>FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</id><score>0.4727388</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Prenyltransferases from the flavedo of Citrus sinensis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1981</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Prenyltransferases from the flavedo of Citrus sinensis</title><author xml:id="author-1"><persName><forename type="first">Milton</forename><surname>De la Fuente</surname></persName><affiliation>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</affiliation></author><author xml:id="author-2"><persName><forename type="first">Luz M.</forename><surname>Pérez</surname></persName></author><author xml:id="author-3"><persName><forename type="first">Uta</forename><surname>Hashagen</surname></persName></author><author xml:id="author-4"><persName><forename type="first">Liliana</forename><surname>Chayet</surname></persName></author><author xml:id="author-5"><persName><forename type="first">Cecilia</forename><surname>Rojas</surname></persName></author><author xml:id="author-6"><persName><forename type="first">Gloria</forename><surname>Portilla</surname></persName><affiliation>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</affiliation></author><author xml:id="author-7"><persName><forename type="first">Osvaldo</forename><surname>Cori</surname></persName></author></analytic><monogr><title level="j">Phytochemistry</title><title level="j" type="abbrev">PHYTO</title><idno type="pISSN">0031-9422</idno><idno type="PII">S0031-9422(00)X0405-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981"></date><biblScope unit="volume">20</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1551">1551</biblScope><biblScope unit="page" to="1557">1557</biblScope></imprint></monogr><idno type="istex">FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</idno><idno type="DOI">10.1016/S0031-9422(00)98529-7</idno><idno type="PII">S0031-9422(00)98529-7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1981</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Citrus sinensis</term></item><item><term>Rutaceae</term></item><item><term>prenyltransferases</term></item><item><term>neryl pyrophosphate</term></item><item><term>geranyl pyrophosphate</term></item><item><term>farnesol</term></item><item><term>stereospecificity.</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>IPP, isopentenyl pyrophosphate (C5)</term></item><item><term>DMAPP, 3,3′-dimethyl pyrophospate (C5)</term></item><item><term>GPP, geranyl pyrophospate (C10E)</term></item><item><term>NPP, neryl pyrophospate (C10Z)</term></item><item><term>(2E,6E)-FPP, (2E,6E)-farnesyl pyrophospate (C15)</term></item><item><term>(2Z,6E)-FPP, (2Z,6E)-farnesyl pyrophospate (C15)</term></item><item><term>DTT, dithiothreithol</term></item><item><term>PCMB</term></item><item><term>para-chloromercuribenzoate</term></item><item><term>DTNB, 5,5′-dithiobis(nitrobenzoic acid):</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1980-09-26">Modified</change><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>PHYTO</jid><aid>00985297</aid><ce:pii>S0031-9422(00)98529-7</ce:pii><ce:doi>10.1016/S0031-9422(00)98529-7</ce:doi><ce:copyright type="unknown" year="1981"></ce:copyright></item-info><head><ce:title>Prenyltransferases from the flavedo of <ce:italic>Citrus sinensis</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Milton</ce:given-name><ce:surname>De la Fuente</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Luz M.</ce:given-name><ce:surname>Pérez</ce:surname></ce:author><ce:author><ce:given-name>Uta</ce:given-name><ce:surname>Hashagen</ce:surname></ce:author><ce:author><ce:given-name>Liliana</ce:given-name><ce:surname>Chayet</ce:surname></ce:author><ce:author><ce:given-name>Cecilia</ce:given-name><ce:surname>Rojas</ce:surname></ce:author><ce:author><ce:given-name>Gloria</ce:given-name><ce:surname>Portilla</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Osvaldo</ce:given-name><ce:surname>Cori</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratorio de Bioquímica General, Facultad de Ciencias Químicas y Farmacológicas, Universidad de Chile, Casilla 233, Santiago 1, Chile</ce:textfn></ce:affiliation><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</ce:textfn></ce:affiliation></ce:author-group><ce:date-revised day="26" month="9" year="1980"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of <ce:italic>Citrus sinensis</ce:italic> with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2<ce:italic>E</ce:italic>,6<ce:italic>E</ce:italic>)- and (2<ce:italic>Z</ce:italic>,6<ce:italic>E</ce:italic>)-farnesyl pyrophosphate. The two C<ce:inf>15</ce:inf>-products are predominant. The <ce:italic>E</ce:italic>- and <ce:italic>Z</ce:italic>-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of <ce:italic>Z</ce:italic>-synthetase were obtained. Mg<ce:sup>2 +</ce:sup> is required for full activity. Mn<ce:sup>2 +</ce:sup> or Co<ce:sup>2 +</ce:sup> can replace Mg<ce:sup>2 +</ce:sup>. The ratio of <ce:italic>E/Z</ce:italic>-products formed is different for each cation. Mg<ce:sup>2 +</ce:sup> complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of <ce:italic>E</ce:italic>- and <ce:italic>Z</ce:italic>-products.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Citrus sinensis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Rutaceae</ce:text></ce:keyword><ce:keyword><ce:text>prenyltransferases</ce:text></ce:keyword><ce:keyword><ce:text>neryl pyrophosphate</ce:text></ce:keyword><ce:keyword><ce:text>geranyl pyrophosphate</ce:text></ce:keyword><ce:keyword><ce:text>farnesol</ce:text></ce:keyword><ce:keyword><ce:text>stereospecificity.</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>IPP, isopentenyl pyrophosphate (C<ce:inf>5</ce:inf>)</ce:text></ce:keyword><ce:keyword><ce:text>DMAPP, 3,3′-dimethyl pyrophospate (C<ce:inf>5</ce:inf>)</ce:text></ce:keyword><ce:keyword><ce:text>GPP, geranyl pyrophospate (C<ce:inf>10</ce:inf><ce:italic>E</ce:italic>)</ce:text></ce:keyword><ce:keyword><ce:text>NPP, neryl pyrophospate (C<ce:inf>10</ce:inf><ce:italic>Z</ce:italic>)</ce:text></ce:keyword><ce:keyword><ce:text>(2<ce:italic>E</ce:italic>,6<ce:italic>E</ce:italic>)-FPP, (2<ce:italic>E</ce:italic>,6<ce:italic>E</ce:italic>)-farnesyl pyrophospate (C<ce:inf>15</ce:inf>)</ce:text></ce:keyword><ce:keyword><ce:text>(2<ce:italic>Z</ce:italic>,6<ce:italic>E</ce:italic>)-FPP, (2<ce:italic>Z</ce:italic>,6<ce:italic>E</ce:italic>)-farnesyl pyrophospate (C<ce:inf>15</ce:inf>)</ce:text></ce:keyword><ce:keyword><ce:text>DTT, dithiothreithol</ce:text></ce:keyword><ce:keyword><ce:text>PCMB</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>para</ce:italic>-chloromercuribenzoate</ce:text></ce:keyword><ce:keyword><ce:text>DTNB, 5,5′-dithiobis(nitrobenzoic acid):</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Prenyltransferases from the flavedo of Citrus sinensis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Prenyltransferases from the flavedo of</title></titleInfo><name type="personal"><namePart type="given">Milton</namePart><namePart type="family">De la Fuente</namePart><affiliation>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1, Chile</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Luz M.</namePart><namePart type="family">Pérez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Uta</namePart><namePart type="family">Hashagen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Liliana</namePart><namePart type="family">Chayet</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Cecilia</namePart><namePart type="family">Rojas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gloria</namePart><namePart type="family">Portilla</namePart><affiliation>Departmento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 233, Santiago 1, Chile</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Osvaldo</namePart><namePart type="family">Cori</namePart><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><dateModified encoding="w3cdtf">1980-09-26</dateModified><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.</abstract><subject><genre>Keywords</genre><topic>Citrus sinensis</topic><topic>Rutaceae</topic><topic>prenyltransferases</topic><topic>neryl pyrophosphate</topic><topic>geranyl pyrophosphate</topic><topic>farnesol</topic><topic>stereospecificity.</topic></subject><subject><genre>Abbreviations</genre><topic>IPP, isopentenyl pyrophosphate (C5)</topic><topic>DMAPP, 3,3′-dimethyl pyrophospate (C5)</topic><topic>GPP, geranyl pyrophospate (C10E)</topic><topic>NPP, neryl pyrophospate (C10Z)</topic><topic>(2E,6E)-FPP, (2E,6E)-farnesyl pyrophospate (C15)</topic><topic>(2Z,6E)-FPP, (2Z,6E)-farnesyl pyrophospate (C15)</topic><topic>DTT, dithiothreithol</topic><topic>PCMB</topic><topic>para-chloromercuribenzoate</topic><topic>DTNB, 5,5′-dithiobis(nitrobenzoic acid):</topic></subject><relatedItem type="host"><titleInfo><title>Phytochemistry</title></titleInfo><titleInfo type="abbreviated"><title>PHYTO</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">1981</dateIssued></originInfo><identifier type="ISSN">0031-9422</identifier><identifier type="PII">S0031-9422(00)X0405-0</identifier><part><date>1981</date><detail type="volume"><number>20</number><caption>vol.</caption></detail><detail type="issue"><number>7</number><caption>no.</caption></detail><extent unit="issue pages"><start>1471</start><end>1766</end></extent><extent unit="pages"><start>1551</start><end>1557</end></extent></part></relatedItem><identifier type="istex">FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</identifier><identifier type="DOI">10.1016/S0031-9422(00)98529-7</identifier><identifier type="PII">S0031-9422(00)98529-7</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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