Endo‐1,4‐β‐glucanase gene expression and cell wall hydrolase activities during abscission in Valencia orange
Identifieur interne : 000151 ( Istex/Corpus ); précédent : 000150; suivant : 000152Endo‐1,4‐β‐glucanase gene expression and cell wall hydrolase activities during abscission in Valencia orange
Auteurs : Jacqueline K. Burns ; Dennis J. Lewandowski ; C. Joseph Nairn ; G. E. BrownSource :
- Physiologia Plantarum [ 0031-9317 ] ; 1998-02.
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Abstract
The physiological and molecular events of ethylene‐induced abscission in mature fruit calyx, laminar and floral abscission zones of cv. Valencia orange were examined. Continuous exposure of fruit explants to 5 µl 1−1 ethylene for 2 to 40 h resulted in marked increases in endo‐1,4‐β‐glucanase (cellulase) and polygalacturonase (PG) activities in calyx abscission zones. Two abscission‐related cellulases and one PG were found. The major peak of cellulase activity corresponded to a pI of 8.0 and molecular weight of 51 kDa, whereas the minor cellulase peak had a pI of 5.5. The abscission polygalacturonase had a pI of 5.5. Calyx abscission zone RNA was amplified with degenerate primers based on sequence of the purified Valencia orange calyx abscission cellulase, and cloned. The two partial cellulase cDNA clones were 59% identical at the nucleotide level. Genomic Southern analysis suggested that Valencia orange contained two groups of cellulase genes. A full‐length cDNA clone from each group was isolated from a cDNA library prepared from ethylene‐induced calyx abscission zone mRNA. Both genes were expressed in ethylene‐induced calyx, laminar and floral abscission zones, but were not expressed in non‐induced abscission zones or mature leaves treated with or without ethylene, young bark or young fruit of Valencia.
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DOI: 10.1034/j.1399-3054.1998.1020209.x
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Joseph</givenNames><familyName>Nairn</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>G. E.</givenNames><familyName>Brown</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="GE"><unparsedAffiliation> J. K. Burns (correspanding author, e‐mail <email>jkbu@icon.lal.ufl.edu</email>) and D. J. Lewandowski, Univ. of Florida, and G. E. Brown, Florida Dept of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA; C. J. Nairn, Univ. of Georgia, Dept of Botany, 2502 Plant Sciences, Athens, GA 30602, USA.</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">Abscission</keyword><keyword xml:id="k2">calyx abscission zone</keyword><keyword xml:id="k3">cellulase activity</keyword><keyword xml:id="k4">cellulase genes</keyword><keyword xml:id="k5">cell wall hydrolase</keyword><keyword xml:id="k6"><i>Citrus sinensis</i></keyword><keyword xml:id="k7">endo‐1,4‐β‐glucanase</keyword><keyword xml:id="k8">floral abscission zone</keyword><keyword xml:id="k9">laminar abscission zone</keyword><keyword xml:id="k10">polygalacturonase activity</keyword><keyword xml:id="k11">Valencia orange</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The physiological and molecular events of ethylene‐induced abscission in mature fruit calyx, laminar and floral abscission zones of cv. Valencia orange were examined. Continuous exposure of fruit explants to 5 µl 1<sup>−1</sup> ethylene for 2 to 40 h resulted in marked increases in endo‐1,4‐β‐glucanase (cellulase) and polygalacturonase (PG) activities in calyx abscission zones. Two abscission‐related cellulases and one PG were found. The major peak of cellulase activity corresponded to a pI of 8.0 and molecular weight of 51 kDa, whereas the minor cellulase peak had a pI of 5.5. The abscission polygalacturonase had a pI of 5.5. Calyx abscission zone RNA was amplified with degenerate primers based on sequence of the purified Valencia orange calyx abscission cellulase, and cloned. The two partial cellulase cDNA clones were 59% identical at the nucleotide level. Genomic Southern analysis suggested that Valencia orange contained two groups of cellulase genes. A full‐length cDNA clone from each group was isolated from a cDNA library prepared from ethylene‐induced calyx abscission zone mRNA. Both genes were expressed in ethylene‐induced calyx, laminar and floral abscission zones, but were not expressed in non‐induced abscission zones or mature leaves treated with or without ethylene, young bark or young fruit of Valencia.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Endo‐1,4‐β‐glucanase gene expression and cell wall hydrolase activities during abscission in Valencia orange</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Endo‐1,4‐β‐glucanase gene expression and cell wall hydrolase activities during abscission in Valencia orange</title></titleInfo><name type="personal"><namePart type="given">Jacqueline K.</namePart><namePart type="family">Burns</namePart><affiliation>J. K. Burns (correspanding author, e‐mail jkbu@icon.lal.ufl.edu) and D. J. Lewandowski, Univ. of Florida, and G. E. Brown, Florida Dept of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA; C. J. Nairn, Univ. of Georgia, Dept of Botany, 2502 Plant Sciences, Athens, GA 30602, USA.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dennis J.</namePart><namePart type="family">Lewandowski</namePart><affiliation>J. K. Burns (correspanding author, e‐mail jkbu@icon.lal.ufl.edu) and D. J. Lewandowski, Univ. of Florida, and G. E. Brown, Florida Dept of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA; C. J. Nairn, Univ. of Georgia, Dept of Botany, 2502 Plant Sciences, Athens, GA 30602, USA.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. Joseph</namePart><namePart type="family">Nairn</namePart><affiliation>J. K. Burns (correspanding author, e‐mail jkbu@icon.lal.ufl.edu) and D. J. Lewandowski, Univ. of Florida, and G. E. Brown, Florida Dept of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA; C. J. Nairn, Univ. of Georgia, Dept of Botany, 2502 Plant Sciences, Athens, GA 30602, USA.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. E.</namePart><namePart type="family">Brown</namePart><affiliation>J. K. Burns (correspanding author, e‐mail jkbu@icon.lal.ufl.edu) and D. J. Lewandowski, Univ. of Florida, and G. E. Brown, Florida Dept of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA; C. J. Nairn, Univ. of Georgia, Dept of Botany, 2502 Plant Sciences, Athens, GA 30602, USA.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Munksgaard International Publishers</publisher><place><placeTerm type="text">Copenhagen</placeTerm></place><dateIssued encoding="w3cdtf">1998-02</dateIssued><edition>Received 15 May, 1997; revised 25 October, 1997</edition><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The physiological and molecular events of ethylene‐induced abscission in mature fruit calyx, laminar and floral abscission zones of cv. Valencia orange were examined. Continuous exposure of fruit explants to 5 µl 1−1 ethylene for 2 to 40 h resulted in marked increases in endo‐1,4‐β‐glucanase (cellulase) and polygalacturonase (PG) activities in calyx abscission zones. Two abscission‐related cellulases and one PG were found. The major peak of cellulase activity corresponded to a pI of 8.0 and molecular weight of 51 kDa, whereas the minor cellulase peak had a pI of 5.5. The abscission polygalacturonase had a pI of 5.5. Calyx abscission zone RNA was amplified with degenerate primers based on sequence of the purified Valencia orange calyx abscission cellulase, and cloned. The two partial cellulase cDNA clones were 59% identical at the nucleotide level. Genomic Southern analysis suggested that Valencia orange contained two groups of cellulase genes. A full‐length cDNA clone from each group was isolated from a cDNA library prepared from ethylene‐induced calyx abscission zone mRNA. Both genes were expressed in ethylene‐induced calyx, laminar and floral abscission zones, but were not expressed in non‐induced abscission zones or mature leaves treated with or without ethylene, young bark or young fruit of Valencia.</abstract><subject lang="en"><genre>keywords</genre><topic>Abscission</topic><topic>calyx abscission zone</topic><topic>cellulase activity</topic><topic>cellulase genes</topic><topic>cell wall hydrolase</topic><topic>Citrus sinensis</topic><topic>endo‐1,4‐β‐glucanase</topic><topic>floral abscission zone</topic><topic>laminar abscission zone</topic><topic>polygalacturonase activity</topic><topic>Valencia orange</topic></subject><relatedItem type="host"><titleInfo><title>Physiologia Plantarum</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0031-9317</identifier><identifier type="eISSN">1399-3054</identifier><identifier type="DOI">10.1111/(ISSN)1399-3054</identifier><identifier type="PublisherID">PPL</identifier><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>102</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>217</start><end>225</end><total>9</total></extent></part></relatedItem><identifier type="istex">DB7B8BA420801B3A971188D7808772BCD583420B</identifier><identifier type="DOI">10.1034/j.1399-3054.1998.1020209.x</identifier><identifier type="ArticleID">PPL1020209</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Munksgaard International Publishers</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/DB7B8BA420801B3A971188D7808772BCD583420B/enrichments/multicat</uri></json:item><json:item><type>refBibs</type><uri>https://api.istex.fr/document/DB7B8BA420801B3A971188D7808772BCD583420B/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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