Cryopreservation of in vitro -grown apical meristems of lily by vitrification
Identifieur interne : 000E98 ( Istex/Checkpoint ); précédent : 000E97; suivant : 000E99Cryopreservation of in vitro -grown apical meristems of lily by vitrification
Auteurs : Toshikazu Matsumoto [Japon] ; Akira Sakai [Japon] ; Kazuto Yamada [Japon]Source :
- Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1995-06-01.
Abstract
Abstract: Apical meristems from adventitious buds induced by culturing of bulb-scale segments of Japanese Pink Lily (Lilium japonicum Thunb.) were successfully cryopreserved by a vitrification. The excised apical meristems were precultured on a solidified Murashige & Skoog medium, containing 0.3 M sucrose, for 1 day at 25°C and then loaded in a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. Cryoprotected meristems were then sufficiently dehydrated with a highly concentrated vitrification solution (designated PVS2) at 25°C for 20 min or at 0°C for 110 min prior to a plunge into liquid nitrogen. After rapid warming in a water bath at 40°C, the meristems were placed in 1.8 ml of 1.2 M sucrose for 20 min and then, placed on filter papers over gellan gum-solidified MS medium. The revived meristems resumed growth within 5 days and directly produced shoots. The rate of shoot formation was approximately 80% after 4 weeks. When bulb-scale segments with adventitious buds were cold-hardened at 0°C for more than 7 days before the procedure, the rates of shoot formation were significantly increased. This vitrification method was successfully applied to five other lily cultivars. Thus, this vitrification procedure for cryopreservation appears promising as a routine method for cryopreserving meristems of lily.
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DOI: 10.1007/BF00045087
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation of in vitro -grown apical meristems of lily by vitrification</title><author><name sortKey="Matsumoto, Toshikazu" sort="Matsumoto, Toshikazu" uniqKey="Matsumoto T" first="Toshikazu" last="Matsumoto">Toshikazu Matsumoto</name></author><author><name sortKey="Sakai, Akira" sort="Sakai, Akira" uniqKey="Sakai A" first="Akira" last="Sakai">Akira Sakai</name></author><author><name sortKey="Yamada, Kazuto" sort="Yamada, Kazuto" uniqKey="Yamada K" first="Kazuto" last="Yamada">Kazuto Yamada</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:52D84C3B405807DA10D1941815C51F805BD3C516</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF00045087</idno><idno type="url">https://api.istex.fr/document/52D84C3B405807DA10D1941815C51F805BD3C516/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000293</idno><idno type="wicri:Area/Istex/Curation">000293</idno><idno type="wicri:Area/Istex/Checkpoint">000E98</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cryopreservation of in vitro -grown apical meristems of lily by vitrification</title><author><name sortKey="Matsumoto, Toshikazu" sort="Matsumoto, Toshikazu" uniqKey="Matsumoto T" first="Toshikazu" last="Matsumoto">Toshikazu Matsumoto</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Shimane Agricultural Experiment Station, Ashiwata 2440, 693, Izumo, Shimane</wicri:regionArea><wicri:noRegion>Shimane</wicri:noRegion></affiliation></author><author><name sortKey="Sakai, Akira" sort="Sakai, Akira" uniqKey="Sakai A" first="Akira" last="Sakai">Akira Sakai</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Asabucho 1-5-23, 001, Kitaku, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Yamada, Kazuto" sort="Yamada, Kazuto" uniqKey="Yamada K" first="Kazuto" last="Yamada">Kazuto Yamada</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Shimane Agricultural Experiment Station, Ashiwata 2440, 693, Izumo, Shimane</wicri:regionArea><wicri:noRegion>Shimane</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Cell, Tissue and Organ Culture</title><title level="j" type="sub">An International Journal on Biotechnology of Higher Plants</title><title level="j" type="abbrev">Plant Cell Tiss Organ Cult</title><idno type="ISSN">0167-6857</idno><idno type="eISSN">1573-5044</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1995-06-01">1995-06-01</date><biblScope unit="volume">41</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="237">237</biblScope><biblScope unit="page" to="241">241</biblScope></imprint><idno type="ISSN">0167-6857</idno></series><idno type="istex">52D84C3B405807DA10D1941815C51F805BD3C516</idno><idno type="DOI">10.1007/BF00045087</idno><idno type="ArticleID">BF00045087</idno><idno type="ArticleID">Art5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-6857</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Apical meristems from adventitious buds induced by culturing of bulb-scale segments of Japanese Pink Lily (Lilium japonicum Thunb.) were successfully cryopreserved by a vitrification. The excised apical meristems were precultured on a solidified Murashige & Skoog medium, containing 0.3 M sucrose, for 1 day at 25°C and then loaded in a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. Cryoprotected meristems were then sufficiently dehydrated with a highly concentrated vitrification solution (designated PVS2) at 25°C for 20 min or at 0°C for 110 min prior to a plunge into liquid nitrogen. After rapid warming in a water bath at 40°C, the meristems were placed in 1.8 ml of 1.2 M sucrose for 20 min and then, placed on filter papers over gellan gum-solidified MS medium. The revived meristems resumed growth within 5 days and directly produced shoots. The rate of shoot formation was approximately 80% after 4 weeks. When bulb-scale segments with adventitious buds were cold-hardened at 0°C for more than 7 days before the procedure, the rates of shoot formation were significantly increased. This vitrification method was successfully applied to five other lily cultivars. Thus, this vitrification procedure for cryopreservation appears promising as a routine method for cryopreserving meristems of lily.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Matsumoto, Toshikazu" sort="Matsumoto, Toshikazu" uniqKey="Matsumoto T" first="Toshikazu" last="Matsumoto">Toshikazu Matsumoto</name></noRegion><name sortKey="Sakai, Akira" sort="Sakai, Akira" uniqKey="Sakai A" first="Akira" last="Sakai">Akira Sakai</name><name sortKey="Yamada, Kazuto" sort="Yamada, Kazuto" uniqKey="Yamada K" first="Kazuto" last="Yamada">Kazuto Yamada</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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