HcPT1.2 participates in Pi acquisition in Hebeloma cylindrosporum external hyphae of ectomycorrhizas under high and low phosphate conditions.
Identifieur interne : 000944 ( Main/Exploration ); précédent : 000943; suivant : 000945HcPT1.2 participates in Pi acquisition in Hebeloma cylindrosporum external hyphae of ectomycorrhizas under high and low phosphate conditions.
Auteurs : Adeline Becquer [France] ; Kevin Garcia [États-Unis] ; Claude Plassard [France]Source :
- Plant signaling & behavior [ 1559-2324 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Pinus.
- métabolisme : Mycorhizes, Phosphates, Phosphore, Pinus.
- physiologie : Hebeloma, Hyphae, Symbiose.
- Régulation de l'expression des gènes fongiques.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Phosphates, Phosphorus.
- metabolism : Mycorrhizae, Pinus.
- microbiology : Pinus.
- physiology : Hebeloma, Hyphae, Symbiosis.
- Gene Expression Regulation, Fungal.
Abstract
Ectomycorrhizal fungi improve tree phosphorus nutrition through transporters specifically localized at soil-hyphae and symbiotic interfaces. In the model symbiosis between the fungus Hebeloma cylindrosporum and the maritime pine (Pinus pinaster), several transporters possibly involved in phosphate fluxes were identified, including three H+:Pi transporters. Among these three, we recently unraveled the function of one of them, named HcPT2, in both pure culture and symbiotic interaction with P. pinaster. Here we investigated the transporter named HcPT1.2, by analyzing inorganic phosphate transport ability in a yeast complementation assay, assessing its expression in the fungus associated or not with the plant, and immunolocalizing the proteins in ectomycorrhizas. We also evaluated the effect of external Pi concentration on expression and localization of HcPT1.2. Our results revealed that HcPT1.2 is involved in Pi acquisition by H. cylindrosporum mycelium, irrespective of the external Pi concentrations.
DOI: 10.1080/15592324.2018.1525997
PubMed: 30289375
PubMed Central: PMC6204789
Affiliations:
Links toward previous steps (curation, corpus...)
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SupAgro , Montpellier , France .</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>a Eco&Sols , University Montpellier, CIRAD, INRA, IRD, Montpellier SupAgro , Montpellier </wicri:regionArea><wicri:noRegion>Montpellier </wicri:noRegion><wicri:noRegion>Montpellier </wicri:noRegion></affiliation></author><author><name sortKey="Garcia, Kevin" sort="Garcia, Kevin" uniqKey="Garcia K" first="Kevin" last="Garcia">Kevin Garcia</name><affiliation wicri:level="2"><nlm:affiliation>b Department of Crop and Soil Sciences , North Carolina State University , Raleigh , NC , USA .</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>b Department of Crop and Soil Sciences , North Carolina State University , Raleigh , NC </wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Plassard, Claude" sort="Plassard, Claude" uniqKey="Plassard C" first="Claude" last="Plassard">Claude 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(metabolism)</term><term>Pinus (microbiology)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Hebeloma (physiologie)</term><term>Hyphae (physiologie)</term><term>Mycorhizes (métabolisme)</term><term>Phosphates (métabolisme)</term><term>Phosphore (métabolisme)</term><term>Pinus (microbiologie)</term><term>Pinus (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Symbiose (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phosphates</term><term>Phosphorus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycorrhizae</term><term>Pinus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Pinus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Pinus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mycorhizes</term><term>Phosphates</term><term>Phosphore</term><term>Pinus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Hebeloma</term><term>Hyphae</term><term>Symbiose</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Hebeloma</term><term>Hyphae</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes fongiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ectomycorrhizal fungi improve tree phosphorus nutrition through transporters specifically localized at soil-hyphae and symbiotic interfaces. In the model symbiosis between the fungus Hebeloma cylindrosporum and the maritime pine (Pinus pinaster), several transporters possibly involved in phosphate fluxes were identified, including three H<sup>+</sup>:Pi transporters. Among these three, we recently unraveled the function of one of them, named HcPT2, in both pure culture and symbiotic interaction with P. pinaster. Here we investigated the transporter named HcPT1.2, by analyzing inorganic phosphate transport ability in a yeast complementation assay, assessing its expression in the fungus associated or not with the plant, and immunolocalizing the proteins in ectomycorrhizas. We also evaluated the effect of external Pi concentration on expression and localization of HcPT1.2. Our results revealed that HcPT1.2 is involved in Pi acquisition by H. cylindrosporum mycelium, irrespective of the external Pi concentrations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30289375</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-2324</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>10</Issue><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Plant signaling & behavior</Title><ISOAbbreviation>Plant Signal Behav</ISOAbbreviation></Journal><ArticleTitle>HcPT1.2 participates in Pi acquisition in Hebeloma cylindrosporum external hyphae of ectomycorrhizas under high and low phosphate conditions.</ArticleTitle><Pagination><MedlinePgn>e1525997</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/15592324.2018.1525997</ELocationID><Abstract><AbstractText>Ectomycorrhizal fungi improve tree phosphorus nutrition through transporters specifically localized at soil-hyphae and symbiotic interfaces. In the model symbiosis between the fungus Hebeloma cylindrosporum and the maritime pine (Pinus pinaster), several transporters possibly involved in phosphate fluxes were identified, including three H<sup>+</sup>:Pi transporters. Among these three, we recently unraveled the function of one of them, named HcPT2, in both pure culture and symbiotic interaction with P. pinaster. Here we investigated the transporter named HcPT1.2, by analyzing inorganic phosphate transport ability in a yeast complementation assay, assessing its expression in the fungus associated or not with the plant, and immunolocalizing the proteins in ectomycorrhizas. We also evaluated the effect of external Pi concentration on expression and localization of HcPT1.2. Our results revealed that HcPT1.2 is involved in Pi acquisition by H. cylindrosporum mycelium, irrespective of the external Pi concentrations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Becquer</LastName><ForeName>Adeline</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>a Eco&Sols , University Montpellier, CIRAD, INRA, IRD, Montpellier SupAgro , Montpellier , France .</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Garcia</LastName><ForeName>Kevin</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0003-0821-1024</Identifier><AffiliationInfo><Affiliation>b Department of Crop and Soil Sciences , North Carolina State University , Raleigh , NC , USA .</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Plassard</LastName><ForeName>Claude</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>a Eco&Sols , University Montpellier, CIRAD, INRA, IRD, Montpellier SupAgro , Montpellier , France .</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>10</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Signal Behav</MedlineTA><NlmUniqueID>101291431</NlmUniqueID><ISSNLinking>1559-2316</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010710">Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>27YLU75U4W</RegistryNumber><NameOfSubstance UI="D010758">Phosphorus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, 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IdType="pubmed">2038328</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="France"><noRegion><name sortKey="Becquer, Adeline" sort="Becquer, Adeline" uniqKey="Becquer A" first="Adeline" last="Becquer">Adeline Becquer</name></noRegion><name sortKey="Plassard, Claude" sort="Plassard, Claude" uniqKey="Plassard C" first="Claude" last="Plassard">Claude Plassard</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Garcia, Kevin" sort="Garcia, Kevin" uniqKey="Garcia K" first="Kevin" last="Garcia">Kevin Garcia</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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