Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.
Identifieur interne : 002E35 ( Main/Exploration ); précédent : 002E34; suivant : 002E36Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.
Auteurs : Aurora Oc N [Allemagne] ; Rüdiger Hampp ; Natalia RequenaSource :
- The New phytologist [ 0028-646X ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Champignons.
- enzymologie : Champignons, Mycorhizes.
- microbiologie : Arbres.
- métabolisme : Disaccharidases, Protéines fongiques, Tréhalose, alpha, alpha-Trehalase.
- Cinétique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Disaccharidases, Fungal Proteins, Trehalase, Trehalose.
- enzymology : Fungi, Mycorrhizae.
- growth & development : Fungi.
- microbiology : Trees.
- Kinetics.
Abstract
Trehalose is a common reserve carbohydrate in fungi, whose role has been recently extended to other cellular functions, such as stress tolerance, glycolysis control, sporulation and infectivity of some pathogenic strains. To gain some insight into the role of trehalose during abiotic stress in arbuscular mycorrhizal (AM) fungi, we assessed trehalose content as well as transcriptional regulation and enzyme activity of neutral trehalase and trehalose-6-phosphate phosphatase in Glomus intraradices in response to heat shock, chemical or osmotic stress. Prolonged or intensive exposure to heat or chemical stress, but not osmotic stress, caused an increase of trehalose in the cell. We found this associated with transient up-regulation of the trehalose-6-P phosphatase (GiTPS2) transcript that coincided with moderate increases in enzyme activity. By contrast, there were no changes in neutral trehalase (GiNTH1) RNA accumulation in response to stress treatments, while they promoted, in most cases, an increase in activity. After stress had ceased, trehalose returned to basal concentrations, pointing to a role of neutral trehalase activity in heat shock recovery. A yeast complementation assay confirmed the role of neutral trehalase in thermotolerance. Taken together, these results indicate that trehalose could play a role in AM fungi during the recovery from certain stresses such as heat shock and chemical treatment.
DOI: 10.1111/j.1469-8137.2007.02048.x
PubMed: 17504469
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.</title><author><name sortKey="Oc N, Aurora" sort="Oc N, Aurora" uniqKey="Oc N A" first="Aurora" last="Oc N">Aurora Oc N</name><affiliation wicri:level="3"><nlm:affiliation>Physiological Ecology of Plants Department, Botanical Institute, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Physiological Ecology of Plants Department, Botanical Institute, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Hampp, Rudiger" sort="Hampp, Rudiger" uniqKey="Hampp R" first="Rüdiger" last="Hampp">Rüdiger Hampp</name></author><author><name sortKey="Requena, Natalia" sort="Requena, Natalia" uniqKey="Requena N" first="Natalia" last="Requena">Natalia Requena</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17504469</idno><idno type="pmid">17504469</idno><idno type="doi">10.1111/j.1469-8137.2007.02048.x</idno><idno type="wicri:Area/Main/Corpus">002F38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002F38</idno><idno type="wicri:Area/Main/Curation">002F38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002F38</idno><idno type="wicri:Area/Main/Exploration">002F38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.</title><author><name sortKey="Oc N, Aurora" sort="Oc N, Aurora" uniqKey="Oc N A" first="Aurora" last="Oc N">Aurora Oc N</name><affiliation wicri:level="3"><nlm:affiliation>Physiological Ecology of Plants Department, Botanical Institute, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Physiological Ecology of Plants Department, Botanical Institute, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Hampp, Rudiger" sort="Hampp, Rudiger" uniqKey="Hampp R" first="Rüdiger" last="Hampp">Rüdiger Hampp</name></author><author><name sortKey="Requena, Natalia" sort="Requena, Natalia" uniqKey="Requena N" first="Natalia" last="Requena">Natalia Requena</name></author></analytic><series><title level="j">The New phytologist</title><idno type="ISSN">0028-646X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disaccharidases (metabolism)</term><term>Fungal Proteins (metabolism)</term><term>Fungi (enzymology)</term><term>Fungi (growth & development)</term><term>Kinetics (MeSH)</term><term>Mycorrhizae (enzymology)</term><term>Trees (microbiology)</term><term>Trehalase (metabolism)</term><term>Trehalose (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (microbiologie)</term><term>Champignons (croissance et développement)</term><term>Champignons (enzymologie)</term><term>Cinétique (MeSH)</term><term>Disaccharidases (métabolisme)</term><term>Mycorhizes (enzymologie)</term><term>Protéines fongiques (métabolisme)</term><term>Tréhalose (métabolisme)</term><term>alpha, alpha-Trehalase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Disaccharidases</term><term>Fungal Proteins</term><term>Trehalase</term><term>Trehalose</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Champignons</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Disaccharidases</term><term>Protéines fongiques</term><term>Tréhalose</term><term>alpha, alpha-Trehalase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Kinetics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trehalose is a common reserve carbohydrate in fungi, whose role has been recently extended to other cellular functions, such as stress tolerance, glycolysis control, sporulation and infectivity of some pathogenic strains. To gain some insight into the role of trehalose during abiotic stress in arbuscular mycorrhizal (AM) fungi, we assessed trehalose content as well as transcriptional regulation and enzyme activity of neutral trehalase and trehalose-6-phosphate phosphatase in Glomus intraradices in response to heat shock, chemical or osmotic stress. Prolonged or intensive exposure to heat or chemical stress, but not osmotic stress, caused an increase of trehalose in the cell. We found this associated with transient up-regulation of the trehalose-6-P phosphatase (GiTPS2) transcript that coincided with moderate increases in enzyme activity. By contrast, there were no changes in neutral trehalase (GiNTH1) RNA accumulation in response to stress treatments, while they promoted, in most cases, an increase in activity. After stress had ceased, trehalose returned to basal concentrations, pointing to a role of neutral trehalase activity in heat shock recovery. A yeast complementation assay confirmed the role of neutral trehalase in thermotolerance. Taken together, these results indicate that trehalose could play a role in AM fungi during the recovery from certain stresses such as heat shock and chemical treatment.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17504469</PMID><DateCompleted><Year>2007</Year><Month>08</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>174</Volume><Issue>4</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.</ArticleTitle><Pagination><MedlinePgn>879-91</MedlinePgn></Pagination><Abstract><AbstractText>Trehalose is a common reserve carbohydrate in fungi, whose role has been recently extended to other cellular functions, such as stress tolerance, glycolysis control, sporulation and infectivity of some pathogenic strains. To gain some insight into the role of trehalose during abiotic stress in arbuscular mycorrhizal (AM) fungi, we assessed trehalose content as well as transcriptional regulation and enzyme activity of neutral trehalase and trehalose-6-phosphate phosphatase in Glomus intraradices in response to heat shock, chemical or osmotic stress. Prolonged or intensive exposure to heat or chemical stress, but not osmotic stress, caused an increase of trehalose in the cell. We found this associated with transient up-regulation of the trehalose-6-P phosphatase (GiTPS2) transcript that coincided with moderate increases in enzyme activity. By contrast, there were no changes in neutral trehalase (GiNTH1) RNA accumulation in response to stress treatments, while they promoted, in most cases, an increase in activity. After stress had ceased, trehalose returned to basal concentrations, pointing to a role of neutral trehalase activity in heat shock recovery. A yeast complementation assay confirmed the role of neutral trehalase in thermotolerance. Taken together, these results indicate that trehalose could play a role in AM fungi during the recovery from certain stresses such as heat shock and chemical treatment.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ocón</LastName><ForeName>Aurora</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Physiological Ecology of Plants Department, Botanical Institute, University of Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hampp</LastName><ForeName>Rüdiger</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Requena</LastName><ForeName>Natalia</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>B8WCK70T7I</RegistryNumber><NameOfSubstance UI="D014199">Trehalose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D004186">Disaccharidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.28</RegistryNumber><NameOfSubstance UI="D014198">Trehalase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.93</RegistryNumber><NameOfSubstance UI="C040631">alpha, alpha-phosphotrehalase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004186" MajorTopicYN="N">Disaccharidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014198" MajorTopicYN="N">Trehalase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014199" MajorTopicYN="N">Trehalose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17504469</ArticleId><ArticleId IdType="pii">NPH2048</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2007.02048.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Tübingen</li></region><settlement><li>Tübingen</li></settlement></list><tree><noCountry><name sortKey="Hampp, Rudiger" sort="Hampp, Rudiger" uniqKey="Hampp R" first="Rüdiger" last="Hampp">Rüdiger Hampp</name><name sortKey="Requena, Natalia" sort="Requena, Natalia" uniqKey="Requena N" first="Natalia" last="Requena">Natalia Requena</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Oc N, Aurora" sort="Oc N, Aurora" uniqKey="Oc N A" first="Aurora" last="Oc N">Aurora Oc N</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E35 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002E35 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17504469
|texte= Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17504469" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |