Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.
Identifieur interne : 003577 ( Main/Exploration ); précédent : 003576; suivant : 003578Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.
Auteurs : Damian S. Bougoure [Australie] ; John W G. CairneySource :
- Environmental microbiology [ 1462-2912 ] ; 2005.
Descripteurs français
- KwdFr :
- Analyse de regroupements (MeSH), Analyse de séquence d'ADN (MeSH), Champignons (génétique), Données de séquences moléculaires (MeSH), Espaceur de l'ADN ribosomique (génétique), Mycorhizes (croissance et développement), Phylogenèse (MeSH), Polymorphisme de restriction (MeSH), Queensland (MeSH), Racines de plante (microbiologie), Rhododendron (microbiologie), Séquence nucléotidique (MeSH), Électrophorèse (MeSH).
- MESH :
- croissance et développement : Mycorhizes.
- génétique : Champignons, Espaceur de l'ADN ribosomique.
- microbiologie : Racines de plante, Rhododendron.
- Analyse de regroupements, Analyse de séquence d'ADN, Données de séquences moléculaires, Phylogenèse, Polymorphisme de restriction, Queensland, Séquence nucléotidique, Électrophorèse.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Cluster Analysis (MeSH), DNA, Ribosomal Spacer (genetics), Electrophoresis (MeSH), Fungi (genetics), Molecular Sequence Data (MeSH), Mycorrhizae (growth & development), Phylogeny (MeSH), Plant Roots (microbiology), Polymorphism, Restriction Fragment Length (MeSH), Queensland (MeSH), Rhododendron (microbiology), Sequence Analysis, DNA (MeSH).
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- geographic : Queensland.
- genetics : Fungi.
- growth & development : Mycorrhizae.
- microbiology : Plant Roots, Rhododendron.
- Base Sequence, Cluster Analysis, Electrophoresis, Molecular Sequence Data, Phylogeny, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA.
Abstract
The culturable fungal assemblage associated with hair roots of Rhododendron lochiae (Ericaceae) from a tropical cloud forest in Queensland, Australia was investigated using rDNA internal transcribed spacer (ITS) restriction fragment length polymorphisms (RFLPs) and sequence analysis, and the abilities of the fungi to form ericoid mycorrhizas were tested. DNA was further extracted directly from hair roots and partial fungal ITS products compared with those from the cultured isolate assemblage using denaturing gradient gel electrophoresis (DGGE). A range of ericoid mycorrhizal and non-mycorrhizal fungi was identified using both approaches, with ericoid mycorrhizal fungi found to be taxonomically similar to those associated with Ericaceae in temperate habitats worldwide. Both approaches identified several unique fungi and, although most of the abundant RFLP types identified in the cultured fungal assemblage were also present in DGGE profiles of DNA extracted directly from roots, one the most commonly isolated RFLP types, a putative Xylariaceae taxon, was absent. The data suggest that a combination of culturing and culture-independent approaches may be more efficacious than either method individually.
DOI: 10.1111/j.1462-2920.2005.00919.x
PubMed: 16232289
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.</title><author><name sortKey="Bougoure, Damian S" sort="Bougoure, Damian S" uniqKey="Bougoure D" first="Damian S" last="Bougoure">Damian S. Bougoure</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Horticulture and Plant Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC AND NSW 1797, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Horticulture and Plant Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC AND NSW 1797</wicri:regionArea><wicri:noRegion>Penrith South DC AND NSW 1797</wicri:noRegion></affiliation></author><author><name sortKey="Cairney, John W G" sort="Cairney, John W G" uniqKey="Cairney J" first="John W G" last="Cairney">John W G. Cairney</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16232289</idno><idno type="pmid">16232289</idno><idno type="doi">10.1111/j.1462-2920.2005.00919.x</idno><idno type="wicri:Area/Main/Corpus">003434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003434</idno><idno type="wicri:Area/Main/Curation">003434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003434</idno><idno type="wicri:Area/Main/Exploration">003434</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.</title><author><name sortKey="Bougoure, Damian S" sort="Bougoure, Damian S" uniqKey="Bougoure D" first="Damian S" last="Bougoure">Damian S. Bougoure</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Horticulture and Plant Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC AND NSW 1797, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Horticulture and Plant Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC AND NSW 1797</wicri:regionArea><wicri:noRegion>Penrith South DC AND NSW 1797</wicri:noRegion></affiliation></author><author><name sortKey="Cairney, John W G" sort="Cairney, John W G" uniqKey="Cairney J" first="John W G" last="Cairney">John W G. Cairney</name></author></analytic><series><title level="j">Environmental microbiology</title><idno type="ISSN">1462-2912</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Electrophoresis (MeSH)</term><term>Fungi (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (growth & development)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (microbiology)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Queensland (MeSH)</term><term>Rhododendron (microbiology)</term><term>Sequence Analysis, DNA (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de regroupements (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Champignons (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Mycorhizes (croissance et développement)</term><term>Phylogenèse (MeSH)</term><term>Polymorphisme de restriction (MeSH)</term><term>Queensland (MeSH)</term><term>Racines de plante (microbiologie)</term><term>Rhododendron (microbiologie)</term><term>Séquence nucléotidique (MeSH)</term><term>Électrophorèse (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Queensland</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Champignons</term><term>Espaceur de l'ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Racines de plante</term><term>Rhododendron</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Rhododendron</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cluster Analysis</term><term>Electrophoresis</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymorphism, Restriction Fragment Length</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Polymorphisme de restriction</term><term>Queensland</term><term>Séquence nucléotidique</term><term>Électrophorèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The culturable fungal assemblage associated with hair roots of Rhododendron lochiae (Ericaceae) from a tropical cloud forest in Queensland, Australia was investigated using rDNA internal transcribed spacer (ITS) restriction fragment length polymorphisms (RFLPs) and sequence analysis, and the abilities of the fungi to form ericoid mycorrhizas were tested. DNA was further extracted directly from hair roots and partial fungal ITS products compared with those from the cultured isolate assemblage using denaturing gradient gel electrophoresis (DGGE). A range of ericoid mycorrhizal and non-mycorrhizal fungi was identified using both approaches, with ericoid mycorrhizal fungi found to be taxonomically similar to those associated with Ericaceae in temperate habitats worldwide. Both approaches identified several unique fungi and, although most of the abundant RFLP types identified in the cultured fungal assemblage were also present in DGGE profiles of DNA extracted directly from roots, one the most commonly isolated RFLP types, a putative Xylariaceae taxon, was absent. The data suggest that a combination of culturing and culture-independent approaches may be more efficacious than either method individually.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16232289</PMID><DateCompleted><Year>2006</Year><Month>05</Month><Day>05</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1462-2912</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>11</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.</ArticleTitle><Pagination><MedlinePgn>1743-54</MedlinePgn></Pagination><Abstract><AbstractText>The culturable fungal assemblage associated with hair roots of Rhododendron lochiae (Ericaceae) from a tropical cloud forest in Queensland, Australia was investigated using rDNA internal transcribed spacer (ITS) restriction fragment length polymorphisms (RFLPs) and sequence analysis, and the abilities of the fungi to form ericoid mycorrhizas were tested. DNA was further extracted directly from hair roots and partial fungal ITS products compared with those from the cultured isolate assemblage using denaturing gradient gel electrophoresis (DGGE). A range of ericoid mycorrhizal and non-mycorrhizal fungi was identified using both approaches, with ericoid mycorrhizal fungi found to be taxonomically similar to those associated with Ericaceae in temperate habitats worldwide. Both approaches identified several unique fungi and, although most of the abundant RFLP types identified in the cultured fungal assemblage were also present in DGGE profiles of DNA extracted directly from roots, one the most commonly isolated RFLP types, a putative Xylariaceae taxon, was absent. The data suggest that a combination of culturing and culture-independent approaches may be more efficacious than either method individually.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bougoure</LastName><ForeName>Damian S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Centre for Horticulture and Plant Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC AND NSW 1797, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cairney</LastName><ForeName>John W G</ForeName><Initials>JW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004586" MajorTopicYN="N">Electrophoresis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011793" MajorTopicYN="N" Type="Geographic">Queensland</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029793" MajorTopicYN="N">Rhododendron</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>5</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16232289</ArticleId><ArticleId IdType="pii">EMI919</ArticleId><ArticleId IdType="doi">10.1111/j.1462-2920.2005.00919.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Cairney, John W G" sort="Cairney, John W G" uniqKey="Cairney J" first="John W G" last="Cairney">John W G. Cairney</name></noCountry><country name="Australie"><noRegion><name sortKey="Bougoure, Damian S" sort="Bougoure, Damian S" uniqKey="Bougoure D" first="Damian S" last="Bougoure">Damian S. Bougoure</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003577 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003577 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16232289
|texte= Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16232289" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |