Microsatellites for disentangling underground networks: strain-specific identification of Glomus intraradices, an arbuscular mycorrhizal fungus.
Identifieur interne : 002C69 ( Main/Exploration ); précédent : 002C68; suivant : 002C70Microsatellites for disentangling underground networks: strain-specific identification of Glomus intraradices, an arbuscular mycorrhizal fungus.
Auteurs : Natarajan Mathimaran [Suisse] ; Laurent Falquet ; Kurt Ineichen ; Cyril Picard ; Dirk Redecker ; Thomas Boller ; Andres WiemkenSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Allèles (MeSH), Marqueurs génétiques (MeSH), Mycorhizes (classification), Mycorhizes (génétique), Polymorphisme génétique (MeSH), Racines de plante (microbiologie), Répétitions microsatellites (MeSH), Spores fongiques (classification), Spores fongiques (génétique), Techniques de typage mycologique (MeSH).
- MESH :
- génétique : ADN fongique, Mycorhizes, Spores fongiques.
- microbiologie : Racines de plante.
- Allèles, Marqueurs génétiques, Polymorphisme génétique, Répétitions microsatellites, Techniques de typage mycologique.
English descriptors
- KwdEn :
- Alleles (MeSH), DNA, Fungal (genetics), Genetic Markers (MeSH), Microsatellite Repeats (MeSH), Mycological Typing Techniques (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Plant Roots (microbiology), Polymorphism, Genetic (MeSH), Spores, Fungal (classification), Spores, Fungal (genetics).
- MESH :
- chemical , genetics : DNA, Fungal.
- classification : Mycorrhizae, Spores, Fungal.
- genetics : Mycorrhizae, Spores, Fungal.
- microbiology : Plant Roots.
- Alleles, Genetic Markers, Microsatellite Repeats, Mycological Typing Techniques, Polymorphism, Genetic.
Abstract
The underground network of arbuscular mycorrhizal (AM) fungi is decisive for the above-ground diversity of many plant ecosystems, but tools to investigate the population structure of AM fungi are sorely lacking. Here, we present a bioinformatics approach to identify microsatellite markers in the AM fungus Glomus intraradices. Based on 1958 contigs of this fungus, assembled from public databases, we identified 842 microsatellites. One hundred of them were subjected to closer scrutiny by designing flanking primers and performing an extensive screen to identify polymorphic loci. We obtained 18 polymorphic microsatellite markers, and we found that seven out of eight individual single-spore cultures of G. intraradices could readily be identified by at least five allelic differences, as compared to all other strains. Two single-spore cultures, however, nominally originating from completely different locations, displayed identity at all 18 loci, suggesting with 99.999999% probability that they represent a single clone.
DOI: 10.1016/j.fgb.2008.02.009
PubMed: 18406181
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Here, we present a bioinformatics approach to identify microsatellite markers in the AM fungus Glomus intraradices. Based on 1958 contigs of this fungus, assembled from public databases, we identified 842 microsatellites. One hundred of them were subjected to closer scrutiny by designing flanking primers and performing an extensive screen to identify polymorphic loci. We obtained 18 polymorphic microsatellite markers, and we found that seven out of eight individual single-spore cultures of G. intraradices could readily be identified by at least five allelic differences, as compared to all other strains. Two single-spore cultures, however, nominally originating from completely different locations, displayed identity at all 18 loci, suggesting with 99.999999% probability that they represent a single clone.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18406181</PMID><DateCompleted><Year>2008</Year><Month>06</Month><Day>16</Day></DateCompleted><DateRevised><Year>2008</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN><JournalIssue CitedMedium="Internet"><Volume>45</Volume><Issue>6</Issue><PubDate><Year>2008</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Fungal genetics and biology : FG & B</Title><ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation></Journal><ArticleTitle>Microsatellites for disentangling underground networks: strain-specific identification of Glomus intraradices, an arbuscular mycorrhizal fungus.</ArticleTitle><Pagination><MedlinePgn>812-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fgb.2008.02.009</ELocationID><Abstract><AbstractText>The underground network of arbuscular mycorrhizal (AM) fungi is decisive for the above-ground diversity of many plant ecosystems, but tools to investigate the population structure of AM fungi are sorely lacking. Here, we present a bioinformatics approach to identify microsatellite markers in the AM fungus Glomus intraradices. Based on 1958 contigs of this fungus, assembled from public databases, we identified 842 microsatellites. One hundred of them were subjected to closer scrutiny by designing flanking primers and performing an extensive screen to identify polymorphic loci. We obtained 18 polymorphic microsatellite markers, and we found that seven out of eight individual single-spore cultures of G. intraradices could readily be identified by at least five allelic differences, as compared to all other strains. 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