Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices.
Identifieur interne : 002010 ( Main/Exploration ); précédent : 002009; suivant : 002011Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices.
Auteurs : Alicia E. Martinez [Argentine] ; Viviana Chiocchio ; Lo Tai Em ; María A. Rodriguez ; Alicia M. GodeasSource :
- Revista de biologia tropical [ 0034-7744 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Cellules germinales de plante, Pteris.
- physiologie : Glomeromycota, Mycorhizes.
- Spores.
English descriptors
- KwdEn :
- MESH :
- microbiology : Germ Cells, Plant, Pteris.
- physiology : Glomeromycota, Mycorrhizae.
- Spores.
Abstract
A Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC No 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions.
PubMed: 23894951
Affiliations:
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Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Av. Int. Güiraldes s/N. Pabellón II. Ciudad Universitaria. 1428, Buenos Aires, Argentina. martae@bg.fcen.uba.ar</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Departamento de Biodiversidad y Biología Experimental. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Av. Int. Güiraldes s/N. Pabellón II. Ciudad Universitaria. 1428, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Chiocchio, Viviana" sort="Chiocchio, Viviana" uniqKey="Chiocchio V" first="Viviana" last="Chiocchio">Viviana Chiocchio</name></author><author><name sortKey="Em, Lo Tai" sort="Em, Lo Tai" uniqKey="Em L" first="Lo Tai" last="Em">Lo Tai Em</name></author><author><name sortKey="Rodriguez, Maria A" sort="Rodriguez, Maria A" uniqKey="Rodriguez M" first="María A" last="Rodriguez">María A. 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Godeas</name></author></analytic><series><title level="j">Revista de biologia tropical</title><idno type="ISSN">0034-7744</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Germ Cells, Plant (microbiology)</term><term>Glomeromycota (physiology)</term><term>Mycorrhizae (physiology)</term><term>Pteris (microbiology)</term><term>Spores (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules germinales de plante (microbiologie)</term><term>Glomeromycota (physiologie)</term><term>Mycorhizes (physiologie)</term><term>Pteris (microbiologie)</term><term>Spores (MeSH)</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Cellules germinales de plante</term><term>Pteris</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Germ Cells, Plant</term><term>Pteris</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glomeromycota</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Spores</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Spores</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC No 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23894951</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>03</Day></DateCompleted><DateRevised><Year>2013</Year><Month>07</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0034-7744</ISSN><JournalIssue CitedMedium="Print"><Volume>60</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Revista de biologia tropical</Title><ISOAbbreviation>Rev Biol Trop</ISOAbbreviation></Journal><ArticleTitle>Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices.</ArticleTitle><Pagination><MedlinePgn>857-65</MedlinePgn></Pagination><Abstract><AbstractText>A Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC No 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. 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Ciudad Universitaria. 1428, Buenos Aires, Argentina. martae@bg.fcen.uba.ar</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chiocchio</LastName><ForeName>Viviana</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Em</LastName><ForeName>Lo Tai</ForeName><Initials>LT</Initials></Author><Author ValidYN="Y"><LastName>Rodriguez</LastName><ForeName>María A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Godeas</LastName><ForeName>Alicia M</ForeName><Initials>AM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Costa Rica</Country><MedlineTA>Rev Biol Trop</MedlineTA><NlmUniqueID>0404267</NlmUniqueID><ISSNLinking>0034-7744</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055993" MajorTopicYN="N">Germ Cells, Plant</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032495" MajorTopicYN="N">Pteris</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23894951</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><noCountry><name sortKey="Chiocchio, Viviana" sort="Chiocchio, Viviana" uniqKey="Chiocchio V" first="Viviana" last="Chiocchio">Viviana Chiocchio</name><name sortKey="Em, Lo Tai" sort="Em, Lo Tai" uniqKey="Em L" first="Lo Tai" last="Em">Lo Tai Em</name><name sortKey="Godeas, Alicia M" sort="Godeas, Alicia M" uniqKey="Godeas A" first="Alicia M" last="Godeas">Alicia M. Godeas</name><name sortKey="Rodriguez, Maria A" sort="Rodriguez, Maria A" uniqKey="Rodriguez M" first="María A" last="Rodriguez">María A. Rodriguez</name></noCountry><country name="Argentine"><noRegion><name sortKey="Martinez, Alicia E" sort="Martinez, Alicia E" uniqKey="Martinez A" first="Alicia E" last="Martinez">Alicia E. Martinez</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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