MECHANISMS INVOLVED IN MYCORRHIZAL WHEAT PROTECTION AGAINST POWDERY MILDEW.
Identifieur interne : 001880 ( Main/Exploration ); précédent : 001879; suivant : 001881MECHANISMS INVOLVED IN MYCORRHIZAL WHEAT PROTECTION AGAINST POWDERY MILDEW.
Auteurs : G. Mustafa ; B. Tisserant ; B. Randoux ; J. Fontaine ; A Lounes-Hadj Sahraoui ; Ph ReignaultSource :
- Communications in agricultural and applied biological sciences [ 1379-1176 ] ; 2014.
Descripteurs français
- KwdFr :
- Antibiose (MeSH), Ascomycota (physiologie), Feuilles de plante (enzymologie), Feuilles de plante (immunologie), Feuilles de plante (microbiologie), France (MeSH), Glomeromycota (physiologie), Lipoxygenase (immunologie), Maladies des plantes (immunologie), Maladies des plantes (microbiologie), Maladies des plantes (prévention et contrôle), Mycorhizes (physiologie), Myeloperoxidase (immunologie), Protéines végétales (immunologie), Triticum (enzymologie), Triticum (immunologie), Triticum (microbiologie).
- MESH :
- enzymologie : Feuilles de plante, Triticum.
- immunologie : Feuilles de plante, Lipoxygenase, Maladies des plantes, Myeloperoxidase, Protéines végétales, Triticum.
- microbiologie : Feuilles de plante, Maladies des plantes, Triticum.
- physiologie : Ascomycota, Glomeromycota, Mycorhizes.
- prévention et contrôle : Maladies des plantes.
- Antibiose, France.
- Wicri :
- geographic : France.
English descriptors
- KwdEn :
- Antibiosis (MeSH), Ascomycota (physiology), France (MeSH), Glomeromycota (physiology), Lipoxygenase (immunology), Mycorrhizae (physiology), Peroxidase (immunology), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Diseases (prevention & control), Plant Leaves (enzymology), Plant Leaves (immunology), Plant Leaves (microbiology), Plant Proteins (immunology), Triticum (enzymology), Triticum (immunology), Triticum (microbiology).
- MESH :
- chemical , immunology : Lipoxygenase, Peroxidase, Plant Proteins.
- geographic : France.
- enzymology : Plant Leaves, Triticum.
- immunology : Plant Diseases, Plant Leaves, Triticum.
- microbiology : Plant Diseases, Plant Leaves, Triticum.
- physiology : Ascomycota, Glomeromycota, Mycorrhizae.
- prevention & control : Plant Diseases.
- Antibiosis.
Abstract
In France, the Ecophyto 2018 national action plan will set out to reduce the use of pesticides by 50% by 2018, if possible. To achieve this goal, the use of arbuscular mycorrhizal (AM) fungi could be a potential alternative method allowing the control of crop diseases. The inoculation by AM fungi has been demonstrated to protect plants against soil-borne pathogens, but little is known about their effectiveness against aerial pathogens, such as the biotrophic fungus Blumeria graminis f.sp. tritici (Bgt) causing wheat (Triticum aestivum) powdery mildew. In the present study, wheat plants were grown in pots, under controlled conditions. Using various phosphorus (P) concentrations, the effectiveness of three AM inocula (Rhizophagus irregularis (Ri), Funneliformis mosseae (Fm)) and Solrize, a mixture of Ri and Fm) in Orvantis wheat cultivar, were tested. After 42 days of culture, mycorrhizal (M) and non-mycorrhizal (NM) wheat plants were infected by Bgt. A satisfactory mycorrhizal rate was obtained with the phosphorus concentration P/5 (P corresponding to the dose used in wheat fields in = 62 mg/L). Our work shows, for the first time, (i) a protective effect of AM inoculation against wheat powdery mildew, reaching up to 73% with Fm inocula, and (ii) its ability to induce a systemic resistance in wheat. Thereafter, we investigated mechanisms involved in this protection. Control plants, M plants, infected plants by Bgt, and M-infected plants were compared at: (i) cytological level, our results revealed that papillae and whole-fluorescent cells presence was induced, conversely fungal haustorium formation in epidermal cells was reduced within M plants leaves (ii) enzymatic level-by assessing defense enzyme activities (lipoxygenase, peroxidase) known as defense markers were measured 24, 48, 72 and 96 hours after infection (hai). The importance of these activities in the defense pathways induced in wheat by AM fungi will be discussed.
PubMed: 26080475
Affiliations:
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To achieve this goal, the use of arbuscular mycorrhizal (AM) fungi could be a potential alternative method allowing the control of crop diseases. The inoculation by AM fungi has been demonstrated to protect plants against soil-borne pathogens, but little is known about their effectiveness against aerial pathogens, such as the biotrophic fungus Blumeria graminis f.sp. tritici (Bgt) causing wheat (Triticum aestivum) powdery mildew. In the present study, wheat plants were grown in pots, under controlled conditions. Using various phosphorus (P) concentrations, the effectiveness of three AM inocula (Rhizophagus irregularis (Ri), Funneliformis mosseae (Fm)) and Solrize, a mixture of Ri and Fm) in Orvantis wheat cultivar, were tested. After 42 days of culture, mycorrhizal (M) and non-mycorrhizal (NM) wheat plants were infected by Bgt. A satisfactory mycorrhizal rate was obtained with the phosphorus concentration P/5 (P corresponding to the dose used in wheat fields in = 62 mg/L). Our work shows, for the first time, (i) a protective effect of AM inoculation against wheat powdery mildew, reaching up to 73% with Fm inocula, and (ii) its ability to induce a systemic resistance in wheat. Thereafter, we investigated mechanisms involved in this protection. Control plants, M plants, infected plants by Bgt, and M-infected plants were compared at: (i) cytological level, our results revealed that papillae and whole-fluorescent cells presence was induced, conversely fungal haustorium formation in epidermal cells was reduced within M plants leaves (ii) enzymatic level-by assessing defense enzyme activities (lipoxygenase, peroxidase) known as defense markers were measured 24, 48, 72 and 96 hours after infection (hai). The importance of these activities in the defense pathways induced in wheat by AM fungi will be discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26080475</PMID><DateCompleted><Year>2015</Year><Month>07</Month><Day>14</Day></DateCompleted><DateRevised><Year>2015</Year><Month>06</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1379-1176</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>3</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Communications in agricultural and applied biological sciences</Title><ISOAbbreviation>Commun Agric Appl Biol Sci</ISOAbbreviation></Journal><ArticleTitle>MECHANISMS INVOLVED IN MYCORRHIZAL WHEAT PROTECTION AGAINST POWDERY MILDEW.</ArticleTitle><Pagination><MedlinePgn>403-10</MedlinePgn></Pagination><Abstract><AbstractText>In France, the Ecophyto 2018 national action plan will set out to reduce the use of pesticides by 50% by 2018, if possible. To achieve this goal, the use of arbuscular mycorrhizal (AM) fungi could be a potential alternative method allowing the control of crop diseases. The inoculation by AM fungi has been demonstrated to protect plants against soil-borne pathogens, but little is known about their effectiveness against aerial pathogens, such as the biotrophic fungus Blumeria graminis f.sp. tritici (Bgt) causing wheat (Triticum aestivum) powdery mildew. In the present study, wheat plants were grown in pots, under controlled conditions. Using various phosphorus (P) concentrations, the effectiveness of three AM inocula (Rhizophagus irregularis (Ri), Funneliformis mosseae (Fm)) and Solrize, a mixture of Ri and Fm) in Orvantis wheat cultivar, were tested. After 42 days of culture, mycorrhizal (M) and non-mycorrhizal (NM) wheat plants were infected by Bgt. A satisfactory mycorrhizal rate was obtained with the phosphorus concentration P/5 (P corresponding to the dose used in wheat fields in = 62 mg/L). Our work shows, for the first time, (i) a protective effect of AM inoculation against wheat powdery mildew, reaching up to 73% with Fm inocula, and (ii) its ability to induce a systemic resistance in wheat. Thereafter, we investigated mechanisms involved in this protection. Control plants, M plants, infected plants by Bgt, and M-infected plants were compared at: (i) cytological level, our results revealed that papillae and whole-fluorescent cells presence was induced, conversely fungal haustorium formation in epidermal cells was reduced within M plants leaves (ii) enzymatic level-by assessing defense enzyme activities (lipoxygenase, peroxidase) known as defense markers were measured 24, 48, 72 and 96 hours after infection (hai). The importance of these activities in the defense pathways induced in wheat by AM fungi will be discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mustafa</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Tisserant</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Randoux</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Fontaine</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sahraoui</LastName><ForeName>A Lounes-Hadj</ForeName><Initials>AL</Initials></Author><Author ValidYN="Y"><LastName>Reignault</LastName><ForeName>Ph</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Belgium</Country><MedlineTA>Commun Agric Appl Biol Sci</MedlineTA><NlmUniqueID>101200320</NlmUniqueID><ISSNLinking>1379-1176</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.7</RegistryNumber><NameOfSubstance UI="D009195">Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.12</RegistryNumber><NameOfSubstance UI="D008084">Lipoxygenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000898" MajorTopicYN="N">Antibiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005602" MajorTopicYN="N" Type="Geographic">France</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008084" MajorTopicYN="N">Lipoxygenase</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009195" MajorTopicYN="N">Peroxidase</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26080475</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Fontaine, J" sort="Fontaine, J" uniqKey="Fontaine J" first="J" last="Fontaine">J. Fontaine</name><name sortKey="Mustafa, G" sort="Mustafa, G" uniqKey="Mustafa G" first="G" last="Mustafa">G. Mustafa</name><name sortKey="Randoux, B" sort="Randoux, B" uniqKey="Randoux B" first="B" last="Randoux">B. Randoux</name><name sortKey="Reignault, Ph" sort="Reignault, Ph" uniqKey="Reignault P" first="Ph" last="Reignault">Ph Reignault</name><name sortKey="Sahraoui, A Lounes Hadj" sort="Sahraoui, A Lounes Hadj" uniqKey="Sahraoui A" first="A Lounes-Hadj" last="Sahraoui">A Lounes-Hadj Sahraoui</name><name sortKey="Tisserant, B" sort="Tisserant, B" uniqKey="Tisserant B" first="B" last="Tisserant">B. Tisserant</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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