iTRAQ and RNA-Seq Analyses Provide New Insights into Regulation Mechanism of Symbiotic Germination of Dendrobium officinale Seeds (Orchidaceae).
Identifieur interne : 000B21 ( Main/Exploration ); précédent : 000B20; suivant : 000B22iTRAQ and RNA-Seq Analyses Provide New Insights into Regulation Mechanism of Symbiotic Germination of Dendrobium officinale Seeds (Orchidaceae).
Auteurs : Juan Chen [République populaire de Chine] ; Si Si Liu [République populaire de Chine] ; Annegret Kohler [France] ; Bo Yan [République populaire de Chine] ; Hong Mei Luo [République populaire de Chine] ; Xiao Mei Chen [République populaire de Chine] ; Shun Xing Guo [République populaire de Chine]Source :
- Journal of proteome research [ 1535-3907 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ARN (MeSH), Dendrobium (physiologie), Germination (MeSH), Graines (physiologie), Mycorhizes (physiologie), Métabolisme glucidique (MeSH), Métabolisme lipidique (MeSH), Protéines végétales (analyse), Protéomique (méthodes), Régulation de l'expression des gènes au cours du développement (MeSH), Symbiose (MeSH), Transcriptome (MeSH), Transduction du signal (MeSH).
- MESH :
- analyse : Protéines végétales.
- méthodes : Protéomique.
- physiologie : Dendrobium, Graines, Mycorhizes.
- Analyse de séquence d'ARN, Germination, Métabolisme glucidique, Métabolisme lipidique, Régulation de l'expression des gènes au cours du développement, Symbiose, Transcriptome, Transduction du signal.
English descriptors
- KwdEn :
- Carbohydrate Metabolism (MeSH), Dendrobium (physiology), Gene Expression Regulation, Developmental (MeSH), Germination (MeSH), Lipid Metabolism (MeSH), Mycorrhizae (physiology), Plant Proteins (analysis), Proteomics (methods), Seeds (physiology), Sequence Analysis, RNA (MeSH), Signal Transduction (MeSH), Symbiosis (MeSH), Transcriptome (MeSH).
- MESH :
- chemical , analysis : Plant Proteins.
- methods : Proteomics.
- physiology : Dendrobium, Mycorrhizae, Seeds.
- Carbohydrate Metabolism, Gene Expression Regulation, Developmental, Germination, Lipid Metabolism, Sequence Analysis, RNA, Signal Transduction, Symbiosis, Transcriptome.
Abstract
Mycorrhizal fungi colonize orchid seeds and induce germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchid species. However, the molecular changes that occur during orchid seed symbiotic germination remain largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed a comparative transcriptomic and proteomic analysis of the Chinese traditional medicinal orchid Dendrobium officinale to explore the change in protein expression at the different developmental stages during asymbiotic and symbiotic germination and identify the key proteins that regulate the symbiotic germination of orchid seeds. Among 2256 identified plant proteins, 308 were differentially expressed across three developmental stages during asymbiotic and symbiotic germination, and 229 were differentially expressed during symbiotic germination compared to asymbiotic development. Of these, 32 proteins were coup-regulated at both the proteomic and transcriptomic levels during symbiotic germination compared to asymbiotic germination. Our results suggest that symbiotic germination of D. officinale seeds shares a common signaling pathway with asymbiotic germination during the early germination stage. However, compared to asymbiotic germination, fungal colonization of orchid seeds appears to induce higher and earlier expression of some key proteins involved in lipid and carbohydrate metabolism and thus improves the efficiency of utilization of stored substances present in the embryo. This study provides new insight into the molecular basis of orchid seed germination.
DOI: 10.1021/acs.jproteome.6b00999
PubMed: 28488443
Affiliations:
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Le document en format XML
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Guo, Shun Xing" sort="Guo, Shun Xing" uniqKey="Guo S" first="Shun Xing" last="Guo">Shun Xing Guo</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Metabolism (MeSH)</term><term>Dendrobium (physiology)</term><term>Gene Expression Regulation, Developmental (MeSH)</term><term>Germination (MeSH)</term><term>Lipid Metabolism (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Plant Proteins (analysis)</term><term>Proteomics (methods)</term><term>Seeds (physiology)</term><term>Sequence Analysis, RNA (MeSH)</term><term>Signal Transduction (MeSH)</term><term>Symbiosis (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ARN (MeSH)</term><term>Dendrobium (physiologie)</term><term>Germination (MeSH)</term><term>Graines (physiologie)</term><term>Mycorhizes (physiologie)</term><term>Métabolisme glucidique (MeSH)</term><term>Métabolisme lipidique (MeSH)</term><term>Protéines végétales (analyse)</term><term>Protéomique (méthodes)</term><term>Régulation de l'expression des gènes au cours du développement (MeSH)</term><term>Symbiose (MeSH)</term><term>Transcriptome (MeSH)</term><term>Transduction du signal (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Dendrobium</term><term>Graines</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Dendrobium</term><term>Mycorrhizae</term><term>Seeds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Gene Expression Regulation, Developmental</term><term>Germination</term><term>Lipid Metabolism</term><term>Sequence Analysis, RNA</term><term>Signal Transduction</term><term>Symbiosis</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term><term>Germination</term><term>Métabolisme glucidique</term><term>Métabolisme lipidique</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Symbiose</term><term>Transcriptome</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mycorrhizal fungi colonize orchid seeds and induce germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchid species. However, the molecular changes that occur during orchid seed symbiotic germination remain largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed a comparative transcriptomic and proteomic analysis of the Chinese traditional medicinal orchid Dendrobium officinale to explore the change in protein expression at the different developmental stages during asymbiotic and symbiotic germination and identify the key proteins that regulate the symbiotic germination of orchid seeds. Among 2256 identified plant proteins, 308 were differentially expressed across three developmental stages during asymbiotic and symbiotic germination, and 229 were differentially expressed during symbiotic germination compared to asymbiotic development. Of these, 32 proteins were coup-regulated at both the proteomic and transcriptomic levels during symbiotic germination compared to asymbiotic germination. Our results suggest that symbiotic germination of D. officinale seeds shares a common signaling pathway with asymbiotic germination during the early germination stage. However, compared to asymbiotic germination, fungal colonization of orchid seeds appears to induce higher and earlier expression of some key proteins involved in lipid and carbohydrate metabolism and thus improves the efficiency of utilization of stored substances present in the embryo. This study provides new insight into the molecular basis of orchid seed germination.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28488443</PMID><DateCompleted><Year>2018</Year><Month>03</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>06</Month><Day>02</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>iTRAQ and RNA-Seq Analyses Provide New Insights into Regulation Mechanism of Symbiotic Germination of Dendrobium officinale Seeds (Orchidaceae).</ArticleTitle><Pagination><MedlinePgn>2174-2187</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jproteome.6b00999</ELocationID><Abstract><AbstractText>Mycorrhizal fungi colonize orchid seeds and induce germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchid species. However, the molecular changes that occur during orchid seed symbiotic germination remain largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed a comparative transcriptomic and proteomic analysis of the Chinese traditional medicinal orchid Dendrobium officinale to explore the change in protein expression at the different developmental stages during asymbiotic and symbiotic germination and identify the key proteins that regulate the symbiotic germination of orchid seeds. Among 2256 identified plant proteins, 308 were differentially expressed across three developmental stages during asymbiotic and symbiotic germination, and 229 were differentially expressed during symbiotic germination compared to asymbiotic development. Of these, 32 proteins were coup-regulated at both the proteomic and transcriptomic levels during symbiotic germination compared to asymbiotic germination. Our results suggest that symbiotic germination of D. officinale seeds shares a common signaling pathway with asymbiotic germination during the early germination stage. However, compared to asymbiotic germination, fungal colonization of orchid seeds appears to induce higher and earlier expression of some key proteins involved in lipid and carbohydrate metabolism and thus improves the efficiency of utilization of stored substances present in the embryo. This study provides new insight into the molecular basis of orchid seed germination.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Juan</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-5407-8239</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Si Si</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kohler</LastName><ForeName>Annegret</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>UMR 1136 INRA/Université de Lorraine, Interactions Arbres/Micro-organismes, INRA, Institut National de la Recherche Agronomique, Centre INRA de Nancy , Champenoux 54280, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Hong Mei</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xiao Mei</ForeName><Initials>XM</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Shun Xing</ForeName><Initials>SX</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193, P. R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031667" MajorTopicYN="N">Dendrobium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="Y">Germination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050356" MajorTopicYN="N">Lipid Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Orchidaceae</Keyword><Keyword MajorTopicYN="Y">carbohydrate metabolism</Keyword><Keyword MajorTopicYN="Y">defense reaction</Keyword><Keyword MajorTopicYN="Y">mycorrhizal interaction</Keyword><Keyword MajorTopicYN="Y">proteome</Keyword><Keyword MajorTopicYN="Y">seed germination</Keyword><Keyword MajorTopicYN="Y">transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28488443</ArticleId><ArticleId IdType="doi">10.1021/acs.jproteome.6b00999</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>République populaire de Chine</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Juan" sort="Chen, Juan" uniqKey="Chen J" first="Juan" last="Chen">Juan Chen</name></noRegion><name sortKey="Chen, Xiao Mei" sort="Chen, Xiao Mei" uniqKey="Chen X" first="Xiao Mei" last="Chen">Xiao Mei Chen</name><name sortKey="Guo, Shun Xing" sort="Guo, Shun Xing" uniqKey="Guo S" first="Shun Xing" last="Guo">Shun Xing Guo</name><name sortKey="Liu, Si Si" sort="Liu, Si Si" uniqKey="Liu S" first="Si Si" last="Liu">Si Si Liu</name><name sortKey="Luo, Hong Mei" sort="Luo, Hong Mei" uniqKey="Luo H" first="Hong Mei" last="Luo">Hong Mei Luo</name><name sortKey="Yan, Bo" sort="Yan, Bo" uniqKey="Yan B" first="Bo" last="Yan">Bo Yan</name></country><country name="France"><region name="Grand Est"><name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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